Nil Tilapia (Oreochromis niloticus Linnaeus, 1758) Rasyonuna Vitamin E (Alfa-Tokoferol Asetat) Katkısının Bazı Spermatolojik Parametreler ve Malondialdehid Düzeyine Etkisi

Year 2019, Volume: 8 Issue: 2, 203 - 207, 25.12.2019

Şeyma Özer , Seyfettin Gür , Özden Barım

https://doi.org/10.31196/huvfd.667756

Abstract

Bu çalışmada, Nil Tilapia balıklarının rasyonuna vitamin E (Alfa-tokoferol Asetat) katkısının spermatolojik parametreler (sperm motilitesi, yoğunluğu ve pH’sı) ve malondialdehit (MDA) düzeyleri üzerine olan etkisi araştırıldı. Çalışmada her bir deneme grubunda 12 balık olmak üzere toplam 48 tilapia balığı kullanıldı. Balıkların beslenmesi için %32 oranında ham protein içeren bir kontrol rasyonu hazırlandı. Bu rasyona 80 mg/kg, 160 mg/kg ve 240 mg/kg miktarlarında vitamin E ilave edilerek sırasıyla D1, D2 ve D3 nolu deneme rasyonları oluşturuldu. Tilapia balıklarının canlı ağırlıklarının %4’ü oranında hazırlanan rasyon, 3 ay süreyle günde üç öğün halinde 25±1 °C su sıcaklığında verildi. Çalışmanın sonunda, D1 ve D3 gruplarında kontrol grubuna göre MDA düzeylerinin sayısal olarak arttığı ancak bu artışın istatistiksel olarak önemsiz olduğu belirlenmiştir (p>0,05). D2 grubunda sperm motilitesinin ve sperma pH’nın sayısal olarak arttığı, ancak bu artışın istatistiksel olarak önemsiz olduğu (p>0,05) belirlenmiştir.

Keywords

Nil tilapia, Vitamin E, Malondialdehid, Spermatolojik parametreler, Alfa-tokoferol asetat

The Effect of Vitamin E (Alpha-Tocopherol Acetate) Supplements to Nile Tilapia (Oreochromis niloticus Linnaeus, 1758) Ration on Some Spermatological Parameters and Malondialdeyhde Level

Abstract

In this study, the effect of vitamin E (Alpha-tocopherol Acetate) contribution to the ration of Nil Tilapia fish on spermatological parameters (sperm motility, density and pH) and malondialdehyde (MDA) levels were investigated. In the study, a total of 48 tilapia fish, 12 fish in each experimental group, were used. A control diet containing 32% crude protein was prepared for feeding the fish. To this diet, 80 mg/kg, 160 mg/kg and 240 mg/kg amounts of vitamin E were added to form D1, D2 and D3, respectively. The ration prepared at the rate of 4% of the live weights of Tilapia fish was given at 25±1 °C water temperature in three times a day for 3 months. At the end of the study, it was determined that MDA levels were increased numerically in D1 and D3 groups, but this increase was statistically insignificant (p>0.05). It was determined that sperm motility and sperm pH were increased numerically in the D2 group, but this increase was statistically insignificant (p> 0.05).

Keywords

Nile Tilapia, Vitamin E, Malondialdehyde, Spermatological parameters, Alpha-Tocopherol Acetate

References

• Adham KG, Hashem MB, Abu-shabana MB, Kame AH, 2000: Vitamin C deficiency in the catfish. Aquacult Nut, 6, 129-139.
• Agarwal A, Saleh RA, Bedaiwy MA, 2003: Role of reactive oxygen species in the pathophysiology of human reproduction. Fertil Steril, 79, 829-843.
• Akiyama M, 1999: In vivo scavenging effect of ethylcysteine on reactive oxygen species in human semen. Japanese J Urology, 90, 421-428.
• Ashley PJ, 2007: Fish welfare: Current issues in aquaculture. App Anim Behaviour Sci, 104, 199-235.
• Atamanalp M, Bayır A, 2003: Bir dezenfektanın (malahit yeşili) subletal dozlarının gökkuşağı alabalığı (Oncorhynchus mykiss) kan parametreleri üzerine etkileri. Gazi Üniv Eğitim Fak Derg, 23, 177-187.
• Benzie IF, 2003: Evolution of dietary antioxidants. Comp Biochem Physiol A Mol Integr Physiol, 136, 113-126.
• Çoban OE, Keleştemur GT, 2011: Farklı oranlardaki sentetik β-karotenin alabalık (Oncorhynchus mykiss, W. 1792) filetolarında kas karotenoid stabilitesi ve lipid peroksidasyon düzeyine etkileri. FÜ Sağ Bil Vet Derg, 25, 17-21.
• Donnelly ET, Mcclure N, Lewis SE, 1999: Antioxidant supplementation in vitro does not improve human sperm motility. Fertil Steril, 72, 484-495.
• Fang YZ, Yang S, Wu G, 2002: Free radicals, antioxidants and nutrition. Nutrition, 18, 872-879.
• Gammanpıla M, Yakupitiyage A, Bart AN, 2007: Evaluation of the effects of dietary vitamin C, E and Zinc supplementation on reproductive performance of Niletilapia (Oreochromis niloticus). Sri Lanka J Aquat Sci, 12, 39-60.
• Gatta PP, Pirini M, Testi S, Vignola G, Monetti PG, 2000: The influence of different levels of dietary vitamin E on sea bass Dicentrarchus labrax flesh quality. Aquacult Nutr, 6, 47-52.
• Ispır U, Yonar ME, Oz OB, 2011: Effect of dietary vitamin E supplementation on the blood parameters of nile tilapia. J Anim Plant Sci, 21, 566-569.
• Karatepe M, 2004: Simultaneous determination of ascorbic acid and free malondialdehyde in human serum by HPLC/UV. LC-GC North America, 22, 362–365.
• Kashif SM, Zaidi R, Banu N, 2003: Antioxidant potential of vitamins A, E and C in modulating oxidative stress in rat brain. Clinica Chimica Act, 340, 229-233.
• Keleştemur GT, 2012: Gökkuşağı alabalığı yavrularının (Oncorhynchus mykiss, W.1792) diyetlerine katılan β-karotenin doku mda düzeyine etkisi. FÜ Sağ Bil Vet Derg, 26, 61-64.
• Kocabas AM, Gatlin DM, 1999: Dietary vitamin E requirement of hybrid striped bass (Morone chrysops female x M. saxatilis male). Aquacult Nutr, 5, 3-7.
• Lovell T, 1989: Nutrition and Feeding of Fish. An. AVI Book, Published by Van Nostrand Reinhold, NewYork.
• Panigrahi A, Kiron V, Kobayashi T, Puangkaew J, Satoh S, Sugita H, 2004: Immune responses in rainbow trout Oncorhynchus mykiss induced by a potential probiotic bacteria Lactobacillus rhamnosus JCM 1136. Vet Immun Immunopathol, 102, 379-388.
• Roderick E, 2001: International file. Fish Farmer International, 15: 24.
• Santiogo CB, Gonzal AC, 2000: Effect of prepared diet and vitamins A, E and C supplementation on the reproductive performance of cage-reared bighead carp Aristichthys nobilis (Richardson). J App Ichthyology, 16, 8-13.
• Shiau SY, Hsu CY, 2002: Vitamin E sparing effect by dietary vitamin C in juvenile hybrid tilapia, Oreochromis niloticus O. aureus. Aquaculture, 210, 335-342.
• Sönmez M, 2016: Reprodüksiyon, Suni Tohumlama ve Androloji Ders Kitabı. 3. Baskı, Elazığ.
• Stéphan G, Guillaume J, Lamour F, 1995: Lipid peroxidation in turbot (Scophthalmus maximus) tissue: effect of dietary vitamin E and dietary n−6 or n−3 polyunsaturated fatty acids. Aquaculture, 130, 251-268.
• Şahan A, Kurutaş E, Dikel S, 2003: Liver antioxidant systems and lipid peroxidation in sea bass (Dicentrarchus labrax) adopted to fresh water. Turk J Vet Animal Sci, 27, 1261-1267.
• Talas ZS, Orun İ, Ozdemir I, Erdogan K, Alkan A, Yılmaz I, 2008: Antioxidative role of selenium against the toxic effect of heavy metals (Cd+2, Cr+3) on liver of rainbow trout (Oncorhynchus mykiss Walbaum 1792). Fish Physiol and Biochem, 34, 217-222.
• Türk G, Sönmez M, Aydin M, Yüce A, Gür S, Yüksel M, Aksu EH, Aksoy H, 2008: Effects of pomegranate juice consumption on sperm quality, spermatogenic cell density, antioxidant activity and testosterone level in male rats. Clin Nutr, 27, 289-296.
• Wang X, Quinn PJ, 1999: Vitamin E and its function in membranes. Prog Lipid Res, 38, 309-336.
• Winston GW, Giulio RT, 1991: Prooxidant and antioxidant mechanisms in aquatic organisms. Aquatic Toxicol, 19, 137-161.