Sığırlarda Mastitisin Teşhisinde Mikrobiyolojik Kültür ve Çiftlikte-Kültür Yöntemlerinin Karşılaştırılması

Year 2022, Volume: 11 Issue: 2, 185 - 192, 30.12.2022

Halid Tekkal , Yaşar Tel

https://doi.org/10.31196/huvfd.1150847

Abstract

Bu çalışmada, bakteriyel nedenli inek mastitislerinin teşhisinde çiftlikte kültür ve konvansiyonel kültür yöntemlerinin karşılaştırılması, izole edilen bakterilerin bazı anibiyotiklere in vitro duyarlıklıklarının ortaya konulması amaçlandı. Çalışmada kanlı agar, MacConkey agar, Edwards medium ve Columbia CNA agar (CNA) kullanıldı. İn vitro antibiyotik duyarlılığının belirlenmesinde neomisin/basitrasin/tetrasiklin (30 μg/10 IU/30), sefapirin (30 μg), amoksisilin/klavulanik asit (2/1) (30 μg), penisilin (10 IU), tetrasiklin (30 μg), klindamisin (2 μg), kanamisin (30 μg) ve sefalotin (30 μg) diskleri kullanıldı. Konvansiyonal izolasyon ve identifikasyon yöntemlerinin uygulandığı çalışmada, örneklerin %90,3’ünden kültür pozitif sonuç alınırken, %9,7’sinde ise herhangi bir aerobik bakteri üremedi. Kanlı agarda yapılan izolasyonda kültür pozitif örneklerinin %17,9’unda (n: 14) Staphylococcus aureus, %61,6’sında (n: 48) Streptococcus spp., %7,7’sinde (n: 6) E. coli, %6,4’ünde (n: 5) Enterococcus spp., %3,8’inde (n: 3) maya ve %2,6’sında (n: 2) koagulaz negatif stafilokok (KNS) saptandı. MacConkey agara yapılan ekimlerin %60’ında (n: 6) E. coli, %30’unda (n: 3) Enterococcus spp. ve %10’unda (n: 1) maya belirlendi. Edwards mediuma ait ekimlerin %91,5’inde (n: 43) Streptococcus spp., %6,4’ünde (n: 3) Enterococcus spp., %2,1’inden (n: 1) maya ve KNS görüldü. CNA’a yapılan ekimlerin %20’sinden (n: 14) S. aureus, %68,6’sından (n: 48) Streptococcus spp., %4,3’ünden (n: 3) Enterococcus spp., %4,3’ünden (n: 3) maya ve %2,8’inden (n: 2) ise KNS izole edildi. Sonuç olarak, mastitisli inek sütlerinde bazı aerobik bakteriyel patojenlerin saptanmasına yönelik uygulanan çiftlikte kültür yönteminin, konvansiyonel kültür yöntemine benzer sonuçlar verdiği ve bu yöntemin enfeksiyona yönelik koruma ve kontrol programlarında kullanılabileceği kanısına varıldı.

Keywords

Sığır, bakteri, mastitis, izolasyon

Supporting Institution

Harran Üniversitesi Bilimsel Araştırmalar Projeler birimi (Hubap)

Project Number

03/06/2020-E.20027

Thanks

Bu çalışmada desteklerini veren Arş. Gör. Ayfer GÜLLÜ YÜCETEPE’ye teşekkürlerimizi sunarız.

Comparison of On-farm Culture and Conventional Culture Methods in the Diagnosis of Mastitis in Cattle

Abstract

This study aimed to compare the microbiological culture and farm-culture methods used to diagnose mastitis in cattle. In the study, blood agar, MacConkey agar, Edwards medium, and Columbia CNA (CNA) agar were inoculated in the media. The samples were incubated under appropriate conditions to perform microbiological analysis of milk samples. While bacterial isolation and identification were performed in 90.3% (75) of the milk samples examined in the study, it was determined that there was no growth in 9.7% (8) of the milk samples, and two different types of pathogens were found in some samples. In blood agar isolation, 17.9% (n: 14) of the culture positive samples were Staphylococcus aureus, 61.6% (n: 48) Streptococcus spp, E. coli in 7.7% (n: 6), Enterococcus spp. in 6.4% (n: 5), yeast in 3.8% (n: 3) and coagulase-negative staphylococci (CNS) in 2.6% (n: 2). E. coli was detected in 60% (n: 6), Enterococcus spp. in 30% (n: 3), and yeast in 10% (n: 1) of the inoculations on MacConkey agar. Streptococcus spp. was observed in 91.5% (n: 43), Enterococcus spp. in 6.4% (n: 3), and yeast and CNS in 2.1% (n: 1) of the cultures of Edwards medium. S. aureus was isolated from 20% (n: 14), Streptococcus spp. from 68.6% (n: 48), Enterococcus spp. from 4.3% (n: 3), yeast from 4.3% (n: 3) and CNS from 2.8% (n: 2) of CNA inoculations. As a result, it was concluded that the on-farm culture method for detecting some aerobic bacterial pathogens in mastitic cow milk gave similar results to the conventional culture method. This method can be used in infection prevention and control programs.

Keywords

Antibiogram, On-farm culture, Cow, Mastitis

Project Number

03/06/2020-E.20027

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