CARNOSOL ALLEVIATES INFLAMMATION AND BACTERIAL TRANSLOCATION IN A RAT MODEL OF INTESTINAL ISCHEMIA-REPERFUSION INJURY

Yıl 2019, Cilt: 5 Sayı: 2, 105 - 118, 27.06.2019

Özgür Cem Müsri , Serhat Oğuz Şaban Gürcan Taner Özgürtaş Tülin Yalta Gokhan Tazegul , Fatma Yalçın Müsri

Öz

Intestinal ischemia-reperfusion injury (II/R) is a condition with significant morbidity and mortality. In this study, we aimed to demonstrate the protective effect of carnosol, an anti-oxidant, anti-inflammatory, anti-carcinogenic and anti-microbial agent, on pro-inflammatory cytokines, tissue angiopoietins, histopathological damage and bacterial translocation in rats subjected to II/R injury. Thirty male Sprague-Dawley rats were divided into 3 groups: control group, intestinal ischemia/reperfusion group (II/R) and carnosol group (II/R+C). Ischemia-reperfusion injury was conducted by laparotomy. All rats received 1010 colony forming units/milliliters E.coli 12 hours prior to surgery. II/R+C rats received 3 mg/kg/day carnosol per oral starting from 3 days prior to surgery. After 72 hours of post-surgical follow-up, rats were re-laparotomized for specimen collection. II/R+C group had lower serum TNF-α, IL-1β and IL-6 levels when compared with II/R group (p=0.026, 0.006 and 0.045, respectively). II/R group had significantly higher tissue injury when compared with II/R+C group (p<0.001). II/R+C group rats had significantly reduced liver, spleen and mesenteric lymph node culture growths when compared with II/R group. Angiopoietin I and II levels were similar between groups. In conclusion, carnosol is a powerful anti-inflammatory and anti-microbial agent in II/R injury model. We showed effective reduction of pro-inflammatory cytokine profile, improvement of histopathological damage, and prevention of bacterial translocation.

Anahtar Kelimeler

Carnosol, intestinal ischemia-reperfusion, antioxidants

Destekleyen Kurum

Financial funding was obtained from Trakya University Scientific Studies Fund.

Proje Numarası

Financial funding was obtained from Trakya University Scientific Studies Fund.

Teşekkür

Financial funding was obtained from Trakya University Scientific Studies Fund.

KARNOSOL SIÇANLARDA BAĞIRSAK İSKEMİ-REPERFÜZYON MODELİNDE İNFLAMASYON VE BAKTERİYEL TRANSLOKASYONU HAFİFLETİR

Öz

İntestinal iskemi-reperfüzyon hasarı (II/R), önemli morbidite ve mortaliteye sahip bir durumdur. Bu çalışmada, anti-oksidan, anti-enflamatuvar, anti-karsinojenik ve anti-mikrobiyal bir ajan olan karnosolün II/R modeli uygulanan sıçanlarda pro-inflamatuar sitokinler, doku anjiopoietinleri, histopatolojik hasar ve bakteriyel translokasyon üzerindeki koruyucu etkisini göstermek amaçlanmıştır. Otuz erkek Sprague-Dawley sıçanı 3 gruba ayrıldı: kontrol grubu, intestinal iskemi/reperfüzyon grubu (II/R) ve karnosol grubu (II/ R+C). İskemi-reperfüzyon hasarı laparotomi ile sağlandı. Tüm sıçanlara ameliyattan 12 saat önce 1010 koloni oluşturan birim/mililitre E.coli uygulandı. II/R+C grubuna, ameliyattan 3 gün önce başlayarak oral 3 mg/kg/gün karnosol uygulandı. Ameliyat sonrası takipten 72 saat sonra, sıçanlar örnek toplama için yeniden laparotomize edildi. II/R+C grubunun II/R grubuna göre serum TNF-α, IL-1β ve IL-6 düzeyleri anlamlı derecede azalmıştı (sırasıyla p=0.026, 0.006 ve 0.045). II/R grubunun, II/R+C grubuna göre daha şiddetli doku hasarına maruz kaldığı görüldü (p<0.001). II/R+C grubu, II/R grubu ile karşılaştırıldığında, karaciğer, dalak ve mezenterik lenf nodu kültür pozitifliğinin anlamlı düzeyde azalmış olduğu gösterilmiştir. Anjiyopoietin I ve II düzeyleri gruplar arasında benzerdi. Sonuç olarak, karnosol güçlü bir anti-inflamatuar ve anti-mikrobiyal ajandır. Bu çalışmada karnosolun II/R modelinde pro-inflamatuar sitokin profilinin etkili bir şekilde azaltan, histopatolojik hasarı düzelten ve bakteriyel translokasyonu önleyen bir ajan olduğu gösterilmiştir.

Anahtar Kelimeler

Karnosol, intestinal iskemi-reperfüzyon, antioksidan

Proje Numarası

Financial funding was obtained from Trakya University Scientific Studies Fund.

Kaynakça

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