Cytoxic Effect Levels Of Sheep Whey Protein in Colorectal Adenocarcinoma Cell Line (Caco-2)

Year 2022, Volume: 14 Issue: 2, 24 - 29, 29.12.2022

İlhan Sabancılar

https://doi.org/10.56484/iamr.1162647

Abstract

Abstract
Objective: It was aimed to determine the antitumor and antiproliferative activity of whey protein (whey) obtained from sheep colostrum isolation on colorectal cancer cells (Caco-2).
Method: Colostrum was separated into fractions as whey proteins using the isoelectric point. Before cell culture was studied, whey protein was sterilized by a membrane filter. Antitumoral activity levels of different concentrations of lyophilized proteins were measured by MTT viability test on cells and IC50 values were determined.
Results: Sheep whey proteins were incubated with Caco-2 cells for 24, 48 and 72 hours with serial dilutions starting with 3200 µg/ml and decreasing. When incubated with sheep whey Caco-2 cells, cell viability: 66.34% at 24 h; IC50 value of 8.414 µg/ml; IC50 value of 46.61% at 48 h was 5,838 µg/ml; The IC50 value of 4.61% at 72 h was found to be 4,741 µg/ml.
Conclusion: It was concluded that whey proteins obtained from sheep colostrum isolation have apoptotic and anticarcinogenic effects on Caco-2 cancer cell line and significantly inhibit the growth of tumor cells. We think that the results of this study will lead to studies to be carried out in vitro.

Keywords

colorectal adenocarcinoma, cytotoxicity, whey proteins, Colostrum

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