Thermal stability and degradation kinetics of the phenolics of Trigonella-foenum graecum L. leaf extracts

Year 2023, Volume: 7 Issue: 2, 116 - 124, 15.08.2023

Hilal İşleroğlu , İzzet Türker

https://doi.org/10.35860/iarej.1262837

Abstract

In this study, thermal stability and degradation kinetics of the phenolics of the aqueous fenugreek leaf extracts were determined. Thermal degradations of total phenolics, total flavonoids, antioxidant activity and total saponins of the fenugreek leaf extracts were examined at different pH values (3.0, 6.0 and 9.0) and different temperatures (60, 70, 80, 90 and 100°C) for time. Moreover, degradation kinetics of the total phenolics were explained by first-order reaction kinetics. Half-life values, free energy and activation energy of the extracts for total phenolic compounds were calculated. According to the results, the extracts showed better thermal stability at pH 3.0 than the other pH values at the selected temperatures concerning total phenolics, total flavonoids, antioxidant activity and total saponins. The degradation of the total phenolics, total flavonoids and antioxidant activity followed similar trends. The phenolic extract of the fenugreek leaves had high thermal stability. The extract had antioxidant activity despite applying eight hours of thermal treatment at 100°C. Kinetic constants (k) were 0.151-0.435 h-1, 0.181-0.491 h-1 and 0.197-0.634 h-1 at pH 3.0, pH 6.0 and pH 9.0, respectively. Activation and free energy values for the degradation of fenugreek phenolics were calculated in the range of 26.02-29.97 kJ/mol and 109.31-120.07 kJ/mol, respectively. The half-life values of total phenolics treated at 60-100°C were 1.59-4.59 h, 1.41-3.83 h, and 1.09-3.52 h for pH 3.0, 6.0 and 9.0, respectively.

Keywords

Fenugreek leaf, Kinetic modeling, Thermal degradation

Supporting Institution

Tokat Gaziosmanpasa University Scientific Research Projects Committee

Project Number

2020/125

References

• 1. K. Srinivasan, Fenugreek (Trigonella foenum-graecum): A review of health beneficial physiological effects. Food Reviews International, 2006. 22(2): p. 203-224. DOI: 10.1080/87559120600586315
• 2. Devi, B.A., N. Kamalakkannan, and P.S.M. Prince, Supplementation of fenugreek leaves to diabetic rats. Effect on carbohydrate metabolic enzymes in diabetic liver and kidney. Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives, 2003. 17(10): p. 1231-1233. DOI: 10.1002/ptr.1357
• 3. Isleroglu, H., and I. Turker, Ultrasonic-assisted extraction and thermal stability of phytochemicals from fenugreek leaves. Journal of Applied Research on Medicinal and Aromatic Plants, 2022. 30: p. 100390. DOI: 10.1016/j.jarmap.2022.100390
• 4. Gonzalez‐Ortega, R., C.D. Di Mattia, P. Pittia, and P.U. Natasa, Effect of heat treatment on phenolic composition and radical scavenging activity of olive leaf extract at different pH conditions: a spectroscopic and kinetic study. Journal of the Science of Food and Agriculture, 2023. 103(4): p. 2047-2056. DOI: 10.1002/jsfa.12371
• 5. Debelo H., M. Li, and M.G. Ferruzzi, Processing influences on food polyphenol profiles and biological activity. Current Opinion in Food Science, 2020. 32: p. 90-102. DOI: 10.1016/j.cofs.2020.03.001
• 6. Santos, L.G., and V.G. Martins, Recovery of phenolic compounds from purple onion peel using bio‐based solvents: Thermal degradation kinetics and color stability of anthocyanins. Journal of Food Processing and Preservation, 2022. 46(12): p. e17161. DOI: 10.1111/jfpp.17161
• 7. Zapata, J.E., C.T. Sepúlveda, and A.C. Álvarez, Kinetics of the thermal degradation of phenolic compounds from achiote leaves (Bixa orellana L.) and its effect on the antioxidant activity. Food Science and Technology, 2021. 44: p. e30920. DOI: 10.1590/fst.30920
• 8. Padmashree, A., G.K. Sharma, A.D. Semwal, and C. Mahesh, Antioxygenic activity of Solanum nigrum L. leaves in sunflower oil model system and its thermal stability. Food and Nutrition Sciences, 2014. 5(11): p. 1-8. DOI: 10.4236/fns.2014.511113
• 9. Friedman, M., and H.S. Jürgens, Effect of pH on the stability of plant phenolic compounds. Journal of Agricultural and Food Chemistry, 2000. 48(6): p. 2101-2110. DOI: 10.1021/jf990489j
• 10. Liu, Y., Y. Liu, C. Tao, M. Liu, Y. Pan, and Z. Lv, Effect of temperature and pH on stability of anthocyanin obtained from blueberry. Journal of Food Measurement and Characterization, 2018. 12: p. 1744-1753. DOI: 10.1007/s11694-018-9789-1
• 11. Amendola, D., D.M. De Faveri, G. Spigno, Grape marc phenolics: Extraction kinetics, quality and stability of extracts. Journal of Food Engineering, 2010. 97(3): p. 384-392. DOI: 10.1016/j.jfoodeng.2009.10.033
• 12. Singleton, V.L., and J.A. Rossi, Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American journal of Enology and Viticulture, 1965. 16(3): p. 144-158.
• 13. Belguith-Hadriche, O., M. Bouaziz, K. Jamoussi, M.S. Simmonds, A. El Feki, and F. Makni-Ayedi, Comparative study on hypocholesterolemic and antioxidant activities of various extracts of fenugreek seeds. Food Chemistry, 2013. 138(2-3): p. 1448-1453. DOI: 10.1016/j.foodchem.2012.11.003
• 14. Pająk, P., R. Socha, J. Broniek, K. Królikowska, and T. Fortuna, Antioxidant properties, phenolic and mineral composition of germinated chia, golden flax, evening primrose, phacelia and fenugreek. Food Chemistry, 2019. 275: p. 69-76. DOI: 10.1016/j.foodchem.2018.09.081
• 15. Akbari, S., N.H. Abdurahman, and R.M. Yunus, Optimization of saponins, phenolics, and antioxidants extracted from fenugreek seeds using microwave-assisted extraction and response surface methodology as an optimizing tool. Comptes Rendus Chimie, 2019. 22(11-12): p. 714-727. DOI: 10.1016/j.crci.2019.07.007
• 16. Isleroglu, H., and I. Turker, Thermal inactivation kinetics of microencapsulated microbial transglutaminase by ultrasonic spray-freeze drying. LWT - Food Science and Technology, 2019. 101: p. 653-662. DOI: 10.1016/j.lwt.2018.11.091
• 17. Chaaban, H., I. Ioannou, L. Chebil, M. Slimane, C. Gérardin, C. Paris, C. Charbonnel, L. Chekir, and M. Ghoul, Effect of heat processing on thermal stability and antioxidant activity of six flavonoids. Journal of Food Processing and Preservation, 2017. 41(5): p. e13203. DOI: 10.1111/jfpp.13203
• 18. Scibisz, I., S. Kalisz, and M. Mitek, Thermal degradation of anthocyanins in blueberry fruit. Zywnosc Nauka Technologia Jakosc (Poland), 2010. 17(5): p. 56-66.
• 19. Nafiunisa, A., N. Aryanti and D.H. Wardhani, Kinetic study of saponin extraction from Sapindus rarak DC by ultrasound-assisted extraction methods. Bulletin of Chemical Reaction Engineering & Catalysis, 2019. 14(2): p. 468-477.
• 20. Heng, L., J.P., Vincken, K. Hoppe, G.A. Van Koningsveld, K. Decroos, H. Gruppen, M.A.J.S. van Boekel, and A.G.J. Voragen, Stability of pea DDMP saponin and the mechanism of its decomposition. Food Chemistry, 2006. 99(2): p. 326-334. DOI: 10.1016/j.foodchem.2005.07.045,
• 21. Singhal, S., P. Rasane, S. Kaur, J. Singh, and N. Gupta, Thermal degradation kinetics of bioactive compounds in button mushroom (Agaricus bisporus) during tray drying process. Journal of Food Process Engineering, 2020. 43(12): p. e13555. DOI: 10.1111/jfpe.13555
• 22. Nambi, V.E., R.K. Gupta, S. Kumar, and P.C. Sharma, Degradation kinetics of bioactive components, antioxidant activity, colour and textural properties of selected vegetables during blanching. Journal of Food Science and Technology, 2016. 53(7): p. 3073-3082. DOI: 10.1007/s13197-016-2280-2
• 23. Ismail, A., Z.M. Marjan, and C.W. Foong, Total antioxidant activity and phenolic content in selected vegetables. Food Chemistry, 2004. 87(4): p. 581-586. DOI: 10.1016/j.foodchem.2004.01.010
• 24. Turkmen, N., F. Sari, and Y.S. Velioglu, The effect of cooking methods on total phenolics and antioxidant activity of selected green vegetables. Food Chemistry, 2005. 93(4): p. 713-718. DOI: 10.1016/j.foodchem.2004.12.038
• 25. Wani, S.A., S. Bishnoi, and P. Kumar, Ultrasound and microwave assisted extraction of diosgenin from fenugreek seed and fenugreek-supplemented cookies. Journal of Food Measurement and Characterization, 2016. 10(3): p. 527-532. DOI: 10.1007/s11694-016-9331-2
• 26. Vikram, V.B., M.N. Ramesh, and S.G. Prapulla, Thermal degradation kinetics of nutrients in orange juice heated by electromagnetic and conventional methods. Journal of Food Engineering, 2005. 69(1): p. 31-40. DOI: 10.1016/j.jfoodeng.2004.07.013
• 27. Oancea, S., and O. Drăghici, pH and thermal stability of anthocyanin-based optimised extracts of Romanian red onion cultivars. Czech Journal of Food Sciences, 2013. 31(3): p. 283-291. DOI: 10.17221/302/2012-CJFS
• 28. Hubbermann, E.M., A. Heins, H. Stöckmann, and K. Schwarz, Influence of acids, salt, sugars and hydrocolloids on the colour stability of anthocyanin rich black currant and elderberry concentrates. European Food Research and Technology, 2006. 223(1): p. 83-90. DOI: 10.1007/s00217-005-0139-2
• 29. Sarpong, F., X. Yu, C. Zhou, L.P. Amenorfe, J. Bai, B. Wu, and H. Ma, The kinetics and thermodynamics study of bioactive compounds and antioxidant degradation of dried banana (Musa ssp.) slices using controlled humidity convective air drying. Journal of Food Measurement and Characterization, 2018. 12(3): p. 1935-1946. DOI: 10.1007/s11694-018-9809-1
• 30. Zahir, E., T. Saleem, H. Siddiqui, S. Naz, and S.M. Shahid, Kinetic and thermodynamic studies of antioxidant and antimicrobial activities of essential oil of lavendula steochs. Journal of Basic and Applied Sciences, 2015. 11: p. 217-222. DOI: 10.6000/1927