Bioremediation of wastewaters from local textile industries

Year 2018, , 16 - 25, 29.12.2018

Abdullahi Ajao , Sunday Awe

https://doi.org/10.38061/idunas.498825

Cited By: 1

Abstract

 The present study evaluates the bioremediation potential of indigenous
bacterial species isolated from dye-contaminated soil samples from small dyeing
outlet located in Ilorin. The water pollution index was estimated based on the
physicochemical characteristics and heavy metal concentrations of the raw (Day
0) and treated textile wastewater such as pH, biochemical oxygen demand-5,
chemical oxygen demand, total suspended solids and total dissolved solid with
mean values of 8.85±0.45 mg/L, 1200±21.3 mg/L, 2440±31.3 mg/L, 1660±17.2 mg/L
and 2650±28.1 mg/L respectively, similarly, Lead was the most abundant heavy
metal detected in the sample while Cadmium concentration was the lowest with
the mean values of 3.52±0.00 mg/L and 2.18±0.00 mg/L respectively. The bacterial
strain with highest dye decolorization capacity was screened and identified as Bacillus licheniformis ZUL012.The
isolate was consequently used for the bioremediation of the wastewater over a
period of 10 days. The results showed an incredible reduction in the  physiochemical characteristics and heavy
metal concentrations of the textile wastewater in the following ranges
(8.85-6.55), (1200-300) mg/L, (2440-518) mg/L, (1660-666) mg/L and (2650-920)
mg/L with the highest removal efficiency of 75 %, 78 %, 60%, 65%,  recorded for biochemical oxygen demand,
chemical oxygen demand, total suspended solid, total dissolved solid,
respectively while that of heavy metals such as lead, cadmium, chromium and
nickel were 80 %, 60 %, 67 %, 72 % reduction, respectively. Laccase and
Azoreductase activities tend to decrease as the pH gradually moved towards
acidic condition during the bioremediation process. Toxicity of the treated
effluent was assessed using Maize and Bean seed germination test. Conclusively,
these research findings can serve as a framework for the outlet design of
wastewater treatment plant for local textile outlets.

Keywords

Bacillus; Bioremediation; Enzymes; Fourier transformed infrared spectroscopy (FTIR); Gas chromatography-mass spectrometry (GC-MS); Physicochemicals; Textile wastewater.

Bioremediation of wastewaters from local textile industries

Abstract

 The present study evaluates the bioremediation potential of indigenous bacterial species isolated from dye-contaminated soil samples from small dyeing outlet located in Ilorin. The water pollution index was estimated based on the physicochemical characteristics and heavy metal concentrations of the raw (Day 0) and treated textile wastewater such as pH, biochemical oxygen demand-5, chemical oxygen demand, total suspended solids and total dissolved solid with mean values of 8.85±0.45 mg/L, 1200±21.3 mg/L, 2440±31.3 mg/L, 1660±17.2 mg/L and 2650±28.1 mg/L respectively, similarly, Lead was the most abundant heavy metal detected in the sample while Cadmium concentration was the lowest with the mean values of 3.52±0.00 mg/L and 2.18±0.00 mg/L respectively. The bacterial strain with highest dye decolorization capacity was screened and identified as Bacillus licheniformis ZUL012.The isolate was consequently used for the bioremediation of the wastewater over a period of 10 days. The results showed an incredible reduction in the physiochemical characteristics and heavy metal concentrations of the textile wastewater in the following ranges (8.85-6.55), (1200-300) mg/L, (2440-518) mg/L, (1660-666) mg/L and (2650-920) mg/L with the highest removal efficiency of 75 %, 78 %, 60%, 65%, recorded for biochemical oxygen demand, chemical oxygen demand, total suspended solid, total dissolved solid, respectively while that of heavy metals such as lead, cadmium, chromium and nickel were 80 %, 60 %, 67 %, 72 % reduction, respectively. Laccase and Azoreductase activities tend to decrease as the pH gradually moved towards acidic condition during the bioremediation process. Toxicity of the treated effluent was assessed using Maize and Bean seed germination test. Conclusively, these research findings can serve as a framework for the outlet design of wastewater treatment plant for local textile outlets.

Keywords

Bacillus, Bioremediation, Enzymes, Fourier transformed infrared spectroscopy (FTIR)

References

• Adegoke, K.; Bello, O.S., (2015). Dye sequestering using agricultural wastes as adsorbents. Water Resour. Ind., 12: 8-24 (17 pages). http://www.academia.edu/29211637/Dye_sequestration_using_agricultural_wastes_as_adsorbents
• Ajao, A.T.; Adebayo, G.B.; Yakubu, S.E., (2011). Bioremediation of textile industrial effluent using mixed culture of Pseudomonas aeruginosa and Bacillus subtilis immobilized on agar agar in a Bioreactor. J. Microbiol. Biotech. Res., 1(3): 50-56 (7 pages). https://jmbronline.com/index.php/JMBR/article/viewFile/32/32
• APHA (2005) Standard Methods for the Examination of Water and Wastewater. 21st Edition, American Public Health Association/American Water Works Association/Water Environment Federation, Washington DC., USA. http://www.scirp.org/(S(czeh2tfqyw2orz553k1w0r45))/reference/ReferencesPapers.aspx?ReferenceID=1870039
• Awomeso, J.A.; Taiwo, A.M..; Gbadebo, A.M.; Adenowo, J.A., (2010). Studies on the pollution of waterbody by textile Industry effluents in Lagos, Nigeria. J. Appl. Sci. Environ. Sanit, 5: 353-359 (7 pages). https://www.researchgate.net/publication/49604247_Studies_on_the_pollution_of_waterbody_by_textile_effluents_inLagos_Nigeria [Google Scholar]Ayansina S. A.; Olubukola, O. B., (2017) Review A New Strategy for Heavy Metal Polluted Environments: A Review of Microbial Biosorbents. Int. J. Environ. Res. Public Health, 14: 94; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5295344/pdf/ijerph-14-00094.pdfBilal, M.; Iqbal, M.; Hu, H.; Zhang, X., (2016). Mutagenicity and cytotoxicity assessment of biodegraded textile effluent by Ca-alginate encapsulated manganese peroxidase. Biochem. Eng. J., (3):1010-1016 (7 pages). https://www.sciencedirect.com/science/article/pii/S1369703X16300201
• Collins, P. J.; Dobson, A. D. W., (1997). Regulation of laccase gene transcription in Trametes versicolor. Appl. Environ. Microbiol., 63: 3444-3450 (7 pages). https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1389241/
• Das, A.; Mishra, S., (2016). Decolorization of different textile azo dyes using an isolated Enterococcus durrans GM13. Int. J. Curr. Microbiol. App. Sci., 5(7): 676-686 (11 pages). https://www.ijcmas.com/5-7-2016/Adya%20Das%20and%20Susmita%20Mishra.pdf
• Elisangela, F.; Rea, Z.; Fabio, D.G.; Cristiano, R.M.; Regina, D.L., (2009). Biodegradation of textile azo dyes by a facultative Staphylococcus arlettae strain VN-11 using a sequential microaerophilic/aerobic process. Int. Biodeter. Biodegr., 63:280-288 (9 pages). https://www.sciencedirect.com/science/article/pii/S0964830508001807.
• Endeshaw, A.; Birhanu, G.; Zerihun, T.; Misganaw, W., (2017) Application of microorganisms in bioremediation-review J. Environ. Microb., 1(1): 2-9 (8 Pages).https://www.peertechz.com/articles/the-role-of-microorganisms-in-bioremediation-a-review.pdfFawole, M.O.; Oso, B.A., (2001). Laboratory Manual of Microbiology. Revised Edition, Spectrum Books, Ibadan. Kaushik, G.; Thakur, I.S., (2014). Production of laccase and optimization of its production by Bacillus sp. using distillery spent wash as inducer. Bioremediat. J., 18: 28-37 (10 pages). https://www.tandfonline.com/doi/abs/10.1080/10889868.2013.834869
• Kayode-Isola, T.M.; Eniola, K. I. T.; Olayemi A.B.; Igunnugbemi, O.O., (2008). Response of Resident Bacteria of a Crude Oil-Polluted River to Diesel Oil. Amer- Eura. J. Agr., 1:06-09 (5 pages). http://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.525.1697&rep=rep1&type=pdfMahmood, S.; Khalid, A.; Mahmood, T.; Arshad, M.; Ahmad, R., (2015). Potential of newly isolated bacterial strains for simultaneous removal of hexavalent chromium and reactive black-5 azo dye from tannery effluent. J. Chem. Technol. Biotechnol., 88(8): 1506-1513 (8 pages). https://onlinelibrary.wiley.com/doi/abs/10.1002/jctb.3994
• Markandeya , T.; Shukla, S.P.; Mohan, D., (2017). Toxicity of Disperse Dyes and its Removal from Wastewater Using Various Adsorbents: A Review: Res. J. Environ. Toxic., 11: 72-89 (18 pages).https://scialert.net/fulltext/?doi=rjet.2017.72.89Mathiyazhagan, N.; Natarajan, D., (2011). Bioremediation of effluent from magnesite and bauxites and textile mine using Thiobacillus spp and Pseudomonas spp. J. Bioremed. Biodegrad., 2:115. doi: 10.4172/2155-6199.1000115. https://www.omicsonline.org/bioremediation-on-effluents-from-magnesite-and-bauxite-mines-using-thiobacillus-spp-and-pseudomonas-spp-2155-6199.1000115.php?aid=454
• Miller, C.S.; Handley, K.M.; Wrighton, K.C.; Frischkorn, K.R.; Thomas, B.C.; Banfield, J.F., (2013). Short-read assembly of full-length 16S amplicons reveals bacterial diversity in subsurface sediments. PloS one 2013: 8(2):e56018 (8 pages). https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0056018&type=printable
• Leelakriangsak, M.; Borisut, S., (2012) Characterization of the decolorizing activity of azo dyes by Bacillus subtilis azoreductase AzoR1. Song. J. Sci. Technol., 34 (5): 509-516 (8 pages).http://rdo.psu.ac.th/sjstweb/Ar-Press/55-Aug/21.pdfMoosvi, S.; Kher, X.; Madamwar, D., (2007). Isolation, characterization and decolorization of textile dyes by a mixed bacterial consortium JW-2. Dyes Pigm., 74: 723–729 (7 pages). https://www.sciencedirect.com/science/article/abs/pii/S0143720806001574
• Ogunjobi, A.A.; Oyinloye, I.A.; Sanuth, H.A., (2012). Bioremediation of effluent from local textile industry using Bacillus licheniformis. N.Y. Sci. J., 5(12): 29-33 (5 pages). http://www.sciencepub.net/newyork/ny0512/004_11494ny0512_29_33.pdf
• Olukanni, D.O.; Osuntoki, A.A.; Awotula, A.O.; Kalyani, D.C.; Gbenle, G.O.; Govindwar, S.P., (2013). Decolorization of dye house effluent and biodegradation of Congo Red by Bacillus thuringiensis RUN1. J.M.B., 23(6): 843-849 (7 pages). http://www.jmb.or.kr/journal/viewJournal.html?doi=10.4014/jmb.1211.11077
• Pandey, A.; Singh, P.; Iyengar, L., (2007). Bacterial decolourisation and degradation of azo dyes. Int. Biodeterio. Biodegrad., (59): 73-84 (12 pages). https://www.sciencedirect.com/science/article/pii/S0964830506001430
• Prasad, A.S.; Bhaskara, K.V., (2010). Physico-chemical characterization of textile effluents and screening for dye decolourizing Bacteria. Global J. Biotechno. Biochem., 5 : (2): 55-62 (8 pages). https://www.idosi.org/gjbb/gjbb5(2)10/2.pdf
• Rajamohan, N.; Karthikeyan, C., (2004). Fungi Biodegradation of Dye house Effluent and Kinetic Modeling’ Department of Chemical Engineering, Annamalai University, Annamalainagar, Tamilnadu-India. https://eco-web.com/edi/041104.html
• Rajeswari, M.; Bhuvaneswari, V., (2016). Production of extracellular laccase from the newly isolated Bacillus sp. PK4. Afr. J. Biotechno., 15(34): 1813-1826 (14 pages). https://www.ajol.info/index.php/ajb/article/view/144424/134070.
• Salisu, D.; Mustapha, H.B., (2010). Industrial Pollution and Implication on Source of Water Supply in Kano, Nigeria. Int. J. Eng. Technol., 10: 1-32 (32 pages). https://pdfs.semanticscholar.org/6a56/028f93ea903d9af0d8fa1feaf28590f1de09.pdf?_ga=2.18125754.2094683901.1539762785-1709058861.1539762785.
• Samuel, E.A.; Ayobami, O. A., (2011). Evaluation of microbial system for bio-treatment of textile waste effluent in Nigeria. Biodecolourisation and Biodegradation of textile dye. JASEM., 15(1): 79-86 (8 pages). http://www.bioline.org.br/pdf?ja11016
• Saratale, R.G.; Saratale, G,D.; Kalyani, D.C.; Chang, J.S.; Govindwar, S.P., (2009). Enhanced decolorization and biodegradation of textile azo dye Scarlet R by using developed microbial consortium-GR. Bioresour. Technol., 100: 2493-2500 (8 pages). https://www.sciencedirect.com/science/article/pii/S0960852408010699?via%3Dihub.
• Saratale, R.G.; Saratale, G.D.; Chang, J. S.; Govindwar, S.P., (2011). Bacterial decolorization and degradation of azo dyes: a review. J. Taiwan Inst. Chem. Eng., 42:138-157 (9 pages). https://www.sciencedirect.com/science/article/pii/S1876107010001094
• Shah, M.P., (2018). Bioremediation-Wastewater Treatment. J. Bioremediat. Biodegrad., 9: 427. https://www.omicsonline.org/open-access/bioremediationwaste-water-treatment-2155-6199-1000427.pdf
• Singh, G.; Ahuja, N.; Sharma, P.; Capalash, N., (2009). Response surface methodology for the optimized production of an alkalophilic laccase from Ɣ-proteobacterium JB. BioRes., 4(2): 544-553 (10 pages). https://bioresources.cnr.ncsu.edu/BioRes_04/BioRes_04_2_0544_Singh_ASC_Design_Exper_Produc_Laccase_420.pdf
• Singh, R.P.; Singh, P.K.; Singh, R.L., (2014). Bacterial Decolorization of textile azo dye Acid Orange by Staphylococcus hominis RMLRT03. Toxicol. Int., 21:160-166 (7 pages). https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4170557/
• Sondhi, S.; Sharma, P.; George, N.; Chauhan, P. S.; Puri, N.; Gupta, N., (2014). An extracellular thermo-alkai-stable laccase from Bacillus tequilensis SN4, with a potential to biobleach softwood pulp. 3 Biotech., 5(2): 175-185 (11 pages). https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4362739/
• Srinivasan, V.; Bhavan, S.P.; Krishnakumar, J., (2014). Bioremediation of textile dye effluent by Bacillus and Pseudomonas spp. Int. J. Sci. Environ. Techn., 3(6): 2215-2224 (10 pages). http://www.ijset.net/journal/472.pdf
• Sriram, N.; Reetha, D.; Saranraj, P., (2013). Biological Degradation of Reactive Dyes by Using Bacteria Isolated from Dye Effluent Contaminated Soil. Middle-East J. Sci. Res., 17(12): 1695-1700 (6 pages). https://www.idosi.org/mejsr/mejsr17(12)13/11.pdf
• Usman, D.H.; Ibrahim, A.; Abdullahi S., (2012). Potentials of Bacterial isolates in Bioremediation of Petroleum Refinery and effluent Wastewater. J. Appl. Phytotechn. Environ. Sanit., 1(3): 131-138 (8 pages). http://www.academia.edu/1544026/
• Vigneshpriya, D; Shanthi, E., (2015). Physicochemical characterization of textile wastewater. Int. J. Innov. Res. Develop., 4(10): 236. http://www.ijird.com/index.php/ijird/article/viewFile/78944/61342.
• Yaseen, D.A.; Scholz, M., (2017). Comparison of experimental ponds for the treatment of dye wastewater under controlled and semi-natural conditions. Environ. Sci. Poll. Res., 24(19): 16031-16040 (10 pages).https://link.springer.com/article/10.1007/s11356-017-9245-5.
• Wang, F.; Yao, J.; Si, Y.; Chen, H.; Russel, M., (2010). Short time effect of heavy metals in soil microbes. Soil Microb. Biochem., 41: 2031-2037 (7 pages). http://iopscience.iop.org/article/10.1088/1755-1315/113/1/012009/pdf