MOLECULAR CHARACTERIZATION OF DIFFERENT CURRANT TYPES

Year 2017, Volume: 1 Issue: 1, 21 - 26, 21.12.2017

Ayşen Melda Çolak , Fatma Alan

Abstract

When berries are of the case, Strawberry, Raspberry, Blackberry, Currants, Gooseberry Blueberries, Elderberry, Wild spindle, Rosehip, Berberis and Sloe generally come to mind and they are fruit types that are consumed deliciously in the world. Their conservation in fresh, following the harvest, their long storage in cold stores and their marketing bear great importance with respect to other fruits. In DNA isolation of berries, more and qualified DNA is obtained by using fresh leaf tissue than old leaf tissue. In this paper, DNAs isolated with the method of CTAB DNA isolation from the leaf of five different currant types (Jostaberry, Redlake, Goliath, Rovada and Boskopgiant) bought Eymen Plantation in Bursa-Kestel province of Turkey are companed with each other in terms of their quantity and quality. Purity and quantity and DNAs which are obtained from young leaves are determined both by gel electrophoresis measurements and UV spectroscopic one. Whether there happens to be genetic variations between the types according to DNA resource is determined by applying ISSR primer and PCR to the DNAs obtained in the study.

Keywords

Currant, Genetic variations, Molecular marker

References

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