The investıgation of possibility of propolıs as additives in alfaalfa silages

Year 2019, Volume: 3 Issue: 2, 207 - 212, 23.12.2019

Asuman Arslan Duru , Mehmet Barıt Mehmet Uygar Türk Nuray Şahinler

Abstract

This study was
carried out to determine chemical, fermentation, microbiological and sensory
properties of alfalfa silages of propolis added at different levels as
additive. In the study, propolis was added to
alfalfa silages at 0 % (control), 0.5, 1.0 and 2.5 levels. Ensiling period
continued for 75 days. There was no statistically significant difference
between dry matter, crude ash, crude protein, NDF ADF contents and lactic acid
bacteria count (P>0.05). pH and propionic
acid content decreased with the addition of propolis to the silages
(P<0.01). The content of
acetic acid decreased with the 1.0% and 2.5% propolis (P<0.01). Listeria spp., ammonia nitrogen and
butyric acid were not found in silages. The Fleig Score of the groups
containing 0.5% and 1.0% propolis was higher than the other groups (P<0.05).
Sulphite reducing anaerobes, mold and Enterobacteriacaea were found to be below the
detection limit and yeast was observed in the propolis group 0.5% and in only
one sample. At the end of the study, it was concluded that the addition of 1.0 %
propolis could increase the quality of silages.

Keywords

Propolis, alfalafa, silage, additive, quality

References

• Referans1: Acar, Z., Bostan, M., 2016. Değişik doğal katkı maddelerinin yonca silajının kalitesine etkilerinin belirlenmesi. Ondokuz Mayıs Üniversitesi Ziraat Fakültesi Anadolu Tarım Bilim. Derg., 433-440.
• Referans2: Aksoy, Z., Dığrak, M., 2006. Bingöl Yöresinde Toplanan Bal ve Propolisin Antimikrobiyal Etkisi Üzerinde in vitro Araştırmalar. Fırat Üniv. Fen ve Müh. Bil. Dergisi 18 (4), 471-478.
• Referans3: AOAC., 1999. Official Methods of Analysis. Association of Official Analytical Chemists, 16th Ed.,Arlington VA
• Referans4: Bankova, V.S., Castro, S.L., Marcucci, M.C., 2000. Propolis: recent advances in chemistry and plant origin. Apidologie 31: 3-15.
• Referans5: Baytok, E., Aksu, T., Karslı, M.A., Muruz, H., 2005. The effects of formic acid molasses, and inoculant as silage additives on corn silage composition and ruminal fermentation characteristics in sheep. Turk J Vet Anim Sci, 29, 469-479.
• Referans6: Bonvehi, J.S., Coll, F.V., 2000. Study on propolis quality from China and Uruguay. Zeitschrift für Naturforsch C, 55: 778-784.
• Referans7: Castaldo, S., Capasso, F., 2002. Propolis, an old remedy used in modern medicine, Fitoterapia, 73, Suppl 1, S1-S6.
• Referans8: Chemid, 1996. A chemical database sponsored by the National Library of Medicine. Bethesda, MD.
• Referans9: Filya İ, Ashbell G, Weinberg ZG, Hen Y., 2001. Hücre duvarını parçalayıcı enzimlerin yonca silajlarının fermantasyon özellikleri, hücre duvarı kapsamı. ve aerobik stabiliteleri üzerine etkileri. Ankara Üniv Zir Fak Tarım Bilim Derg,7 (3): 81-87, 2001.
• Referans10: Filya, I., Muck, R. E., Contreras-Govea, F. E., 2006. Inoculant Effects on Alfalfa Silage: Fermentation Products and Nutritive Value. J. Dairy Sci. 90:5108–5114.
• Referans11: Gallez, L., Kiehr, M., Fernández, L., Delhey, R., Stikar, D., 2014. Antifungal activity in vitro of propolis solutions from Argentina against two plant pathogenic fungi: Didymella bryoniae and Rhizotocnia solani. Journal of Apicultural Research, 53:4, 438-440
• Referans12: Ghisalberti, E. L., 1979. Propolis: A review, Bee World, 60: 59-84.
• Referans13: Grange, J.M., Davey, R.W., 1990. Antibacterial properties of propolis. Journal of the Royal Society of Medicine, Volume 83, March.
• Referans14: Guo, X.S., Ding, W.R., Han, J.G., Zhou, H., 2008. Characterization of protein fractions and amino acids in ensiled alfalfa treated with different chemical additives. Animal Feed Science and Technology. 142: 89–98.
• Referans15: Harrigan, W.F., 1998. Laboratory methods in food microbiology. London, 3nd ed., Academic Pres.
• Referans16: Hepşen, İ.F., Tilgen, F., Hamdi E., 1996. Propolis: Tıbbi Özellikleri ve Oftalmolojik Kullanımı. Turgut Özal Tıp Merkezi Dergisi, 3(4), 386-391.
• Referans17: Ke, W.C., Yang, F.Y., Undersander, D. J., Guo, X.S., 2015. Fermentation characteristics, aerobic stability, proteolysis and lipid composition of alfalfa silage ensiled with apple or grape pomace Animal Feed Science and Technology 202 (2015) 12–19.
• Referans18: Kılıç, A., 1986. Ensilage feed. İzmir, Turkey: Bilgehan Press; 1986.
• Referans19: Koç, F., Aksoy, S.O., Okur, A.A., Celikyurt, G., Korucu, D., & Ozduven, M.L., (2017). Effect of pre-fermented juice, Lactobacillus plantarum and Lactobacillus buchneri on the fermentation characteristics and aerobic stability of high dry matter alfalfa bale silage. The Journal of Animal & Plant Sciences, 27(6), 1766-1773.
• Referans20: Kumova, U., Korkmaz, A., Avcı, B.C., Ceyran, G., 2002. An important bee products: Propolis. Uludag Apiculture J. 2: 10-24.
• Referans21: Liu.,C., Yu-jiao, L., Xiao-nan, L., Ping-ting, G., Hai-ling, L., 2016. Effect of lactic acid bacteria inoculants on alfalfa (Medicago sativa L.) silage quality: assessment of degradation (in situ) and gas production (in vitro). Journal of Integrative Agriculture 2016, 15(12): 2834–2841.
• Referans22: Matsuno, T., Chen, C., Basnet, P., 1997. A tumoricidal and antioxidant compound isolated from an aqueous extract of propolis. Medical Science Research 25, 583–584.
• Referans23: McDonald, P., Henderson, A. R., Herson, S. J. E., 1991. The Biochemistry of Silage. 16th ed., Chalcombe Publication, Marlow, UK.
• Referans24: Meneses, E.A., Durango, D.L., García, C.M., 2009. Antifungal Activity Against Postharvest Fungi by Extracts from Colombian Propolis. Quim. Nova, Vol. 32, No. 8.
• Referans25: Muck, R.E., 2010. Silage microbiology and its control through additives. R. Bras. Zootec. 39: 183-191.
• Referans26: Oruç, H.H., Sorucu, A., Aydın, L., 2014. Propolisin Sağlık Açısından Önemi, Kalitesinin Belirlenmesi ve Türkiye Açısından İrdelenmesi. Uludag Bee Journal, 14(1).
• Referans27: Petterson, K., 1988. Ensiling of Forages: Factors Affecting Silage Fermantation and Quality. Sveriges Lantbruksuniversitet, 46 p,Uppsala.
• Referans28: Polan, C.E., Stive, D.E., Garrett, J.L., 1998. Protein preservation and ruminal degradation of ensiled forage treated with heat, formic acid, ammonia, or microbial inoculant, Journal of Dairy Science, 81, 765-776.
• Referans29: Pour, H.A., Naserian, A.A., Vakili, A.R., Tahmasbi, A.M., (2017). Effect of Essential Plant Oil Used as an Additive to Alter Silage Fermentation in Ruminant by In Vitro. Biosciences Biotechnology Research Asia. 14(1), 145-152.
• Referans30: Stanley, E.R., Bradley, T.R., Sumner, M.A., 1971. Properties of the mouse embryo conditioned medium factor(s) stimulationg colony formation by mouse bone marrow cells grown in vitro. J. Cell. Physiology, 78, 301–317.
• Referans31: Szliszka, E., Czuba, Z.P., Domino, M., Mazur, B., Zydowicz, G., Krol, W., 2009. Ethanolic extract of propolis (EEP) enhances the apoptosisinducing potential of TRAIL in cancer cells Molecules, 14: 738-754.
• Referans32: Şahinler, N., 1999. Propolisin Bileşimi ve Kullanma Olanakları. M.K.Ü. Ziraat Fakültesi Dergisi, 4(1-2), 167-180.
• Referans33: SPSS, 2007. SPSS for Windows Release 16.01. SPSS Incorporation. 2007.
• Referans34: Wen, A., Yuan, X., Wang, J., Desta, S.T., Shao, T., 2017. Effects of four short-chain fatty acids or salts on dynamics of fermentation and microbial characteristics of alfalfa silage. Animal Feed Science and Technology 223:141–148.
• Referans35: Van Soest, P. J., 1982. Analytical systems for evaluation of feeds. In: P. J. Van Soest (Editor), Nutritional Ecology of the Ruminant. Cornell University Press. Chapter 6, pp. 75-94. Ithaca, NY.
• Referans36: Velazquez, C., Navarro, M., Acosta, A., Angulo, A., Dominguez, Z., Roble, R., Robles-Zepeda, R., Lugo, E., Goycoolea, F.M., Velazquez, E.F., 2007. Antibacterial and free-radical scavenging activities of Sonoran propolis. J. Appl. Microbiol, 103: 1747–1756.