Year 2016,
Special Issue (2016), 161 - 164, 01.12.2016
Ayşen Çetin Kardeşler
,
Meriç Çetin
,
Selami Beyhan
References
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Monitoring of Anxiety-Like Behaviors on Rats with Video Tracking Technology
Year 2016,
Special Issue (2016), 161 - 164, 01.12.2016
Ayşen Çetin Kardeşler
,
Meriç Çetin
,
Selami Beyhan
Abstract
Artificial
sweeteners like MSG (Mono Sodium Glutamate) model has been used anxiety-like
behaviors on rats. The tracking of rat’s movements has broad applicability to
questions in anxiety-like behaviors with different doses MSG injections (50 mg/kg/day,
100 mg/kg/day and 200 mg/kg/day) to rats. In this paper, in order to measure
three types locomotor activity (line crossing, rearing, grooming), a video
tracking software is used. The advantage of this type of tracking software is
that it provides to give locomotor activity of rats in real-time. The
experimental results obtained in this study have shown that learning and
memorial functions negatively affected in the brains of the rats an
anxiety-like model. In addition, the visual tracking results demonstrate that
video tracking system provides an accurate monitoring of rat’s behavior.
References
- [1] S. Vinogradov, et al. Is serum brain-derived neurotrophic factor a biomarker for cognitive enhancement in schizophrenia? Biol Psychiatry.; vol. 66, pp. 549-553, 2009.
- [2] P. Ma, X . Liu, J . Wu, B . Yan, Y . Zhang, Y. Lu, Y. Wu, C . Liu, J. Guo, E. Nanberg, C.G. Bornehag, X. Yang. Cognitive deficits and anxiety induced by diisononyl phthalatein mice and the neuroprotective effects of melatonin, Nature. Vol. 5, pp. e14676, 2015.
- [3] M. Dubovicky, D. Tokarev, I. Skultetyova, D. Jezova. Changes of exploratory behavior and its habituation in rats neonatally treated with monosodium glutamate, Pharmacol Biochem Behav, vol, 56, pp. 565-569, 1997.
- [4] Z. Hlinak, D. Gandalovicova & I. Krejci. Behavioral deficits in adult rats treated neonatally with glutamate, Neurotoxicol Teratol, vol. 27, pp. 465–473, 2005.
- [5] N. Enginar, I. Hatipoglu, ˘ M. Firtina, Evaluation of the acute effects of amitriptyline and fluoxetine on anxiety using grooming analysis algorithm in rats, Pharmacol. Biochem. Behav. Vol. 89, pp. 450–455, 2008.
- [6] T.S. Perrot-Sinal, A. Gregus, D. Boudreau, L.E. Kalynchuk, Sex and repeated restraint stress interact to affect cat odor-induced defensive behavior in adult rats, Brain Res., vol. 1027, pp. 161–172, 2004.
- [7] A. Takeda, H. Tamano, F. Kan, H. Itoh, N. Oku, Anxiety-like behavior of young rats after 2-week zinc deprivation, Behav. Brain Res., vol. 177, pp. 1–6, 2007.
- [8] O.J. Onaolapo, A.Y. Onaolapo, M.A. Akanmu, G. Olayiwola, Foraging enrichment modulates open field response to monosodium glutamate in mice. Annals of neurosciences, vol. 22.3, pp. 162, 2015.
- [9] P. Muigg, S. Scheiber, P. Salchner, M. Bunck, R. Landgraf, N. Singewald, Differential stress-induced neuronal activation patterns in mouse lines selectively bred for high, normal or low anxiety, PLoS One vol. 4, e5346, 2009.
- [10] RJ. Beninger, TA. Cooper, EJ. Mazurski, “Automating the measurement of locomotor activity”, Neurobehav Toxicol Teratol, vol. 7, pp. 79–85, 1985.
- [11] RL. Clarke, RF. Smith, DR. Justesen, “An infrared device for detecting locomotor activity”, Behav Res Methods Instrum Comput, vol. 17, pp. 519–525, 1985.
- [12] E. Robles, “A method to analyze the spatial distribution of behavior”, Behav Res Methods Instrum Comput, vol. 22, pp. 540–549, 1990.
- [13] MH. Teicher, SL. Andersen, P. Wallace, DA. Klein, J. Hostetter, Development of an affordable hi-resolution activity monitor system for laboratory animals, Pharmacol Biochem Behav, vol. 54, pp. 479–483, 1996.
- [14] HA. Van de Weerd, RJ. Bulthuis, AF. Bergman, F. Schlingmann, J. Tolboom, PL. Van Loo et al., Validation of a new system for the automatic registration of behaviour in mice and rats. Behav Processes, vol. 53, pp. 11–20, 2001.
- [15] AM. Deveney, A. Kjellstrom, T. Forsberg, DM. Jackson. A pharmacological validation of radiotelemetry in conscious, freely moving rats. J Pharmacol Toxicol Methods, vol. 40, pp. 71–79, 1998.
- [16] V. Pasquali, P. Renzi, On the use of microwave radar devices in chronobiology studies: an application with Periplaneta Americana, Behav Res Methods, vol. 37, pp. 522–527, 2005.
- [17] LP. Noldus, AJ. Spink, RA. Tegelenbosch. EthoVision: a versatile video tracking system for automation of behavioral experiments. Behav Res Methods Instrum Comput, vol. 33, pp. 398–414, 2001.
- [18] CV. Vorhees, KD. Acuff-Smith, DR. Minck, RE. Butcher, A method for measuring locomotor behavior in rodents: contrast-sensitive computer-controlled video tracking activity assessment in rats. Neurotoxicol Teratol, vol. 14, pp. 43–49, 1992.
- [19] Q. Xu, C. Cai, H. Zhou, H. Ren, A video tracking system for limb motion measurement in small animals. In Optoelectronics and Image Processing, International Conference, vol. 1, pp. 181-184, 2010.
- [20] S. C. Fu, K. M. Chan, L. S. Chan, D. T. P Fong, P. Y. P Lui, The use of motion analysis to measure pain-related behaviour in a rat model of degenerative tendon injuries. Journal of neuroscience methods, vol. 179.2, pp. 309-318, 2009.
- [21] JA. Endler, On the measurement and classification of colour in studies of animal colour patterns. Biol J Linn Soc, vol. 41, pp. 315–352, 1990.
- [22] BA. McCool, AM. Chappell. Chronic intermittent ethanol inhalation increases ethanol self-administration in both C57BL/6J and DBA/2J mice. Alcohol., vol. 49, pp. 111-120, 2015.
- [23] CB. Quines, SG. Rosa, JT. Da Rocha, BM. Gai, CF. Bortolatto, MF. Duarte, CW. Nogueira. Monosodium glutamate, a food additive, induces depressive-like and anxiogenic-like behaviors in young rats. Life Sciences, vol. 107, pp. 27-31, 2016.
- [24] SG. Rosa, CB. Quines, EC. Stangherlin, CW. Nogueira. Diphenyl diselenide ameliorates monosodium glutamate induced anxiety-like behavior in rats by modulating hippocampal BDNF-Akt pathway and uptake of GABA and serotonin neurotransmitters. Physiology & Behavior, vol. 155, pp. 1-8, 2016.
- [25] J. Sun, C. Wan, P. Jia, AH. Fanous, KS. Kendler, BP. Riley, et al. Application of systems biology approach identifies and validates GRB2 as a risk gene for schizophrenia in the Irish Case Control Study of Schizophrenia (ICCSS) sample. Schizophrenia research., vol. 125, pp. 201–208, 2011.
- [26] NH. Woo, HK. Teng, CJ. Siao, et al. Activation of p75NTR by proBDNF facilitates hippocampal long-term depression. Nat Neurosci., vol. 8, pp. 1069-1077, 2005.