Evaluation for Resistance Sources of Common Bean Cultivars Grown in Turkey to Rust (Uromyces appendiculatus) and Common Bacterial Blight (Xanthomonas axonopodis pv. phaseoli) Diseases

Year 2021, Volume: 7 Issue: 2, 222 - 230, 25.08.2021

Gülsüm Palacıoğlu , Sinem Tombul Harun Bayraktar , Göksel Özer

https://doi.org/10.24180/ijaws.882996

Cited By: 2

Abstract

Bean rust caused by Uromyces appendiculatus and common bacterial blight by Xanthomonas axonopodis pv. phaseoli are among the important diseases that widely occur in bean production areas worldwide. The most effective and sustainable control method against these disease is the development of resistant breeding materials. Many molecular markers associated with the loci that play a role in resistance against these diseases have been developed and integrated in breeding programs. In this study, 40 common bean cultivars widely grown in Turkey were screened molecularly for resistance sources against bean rust and common bacterial blight diseases using SCAR markers (SAE19, SI19, SA14, SBC6, SAP6, SU91, and BC420). The loci of Ur-11 and Ur-4 associated with resistance against U. appendiculatus were very common among common bean cultivars, while B10 resistance locus against X. axonopodis pv. phaseoli was determined in only Boncuk, Belinay Sırık, Sırık Barbunya, Zülbiye and Bulduk cultivars from common bean cultivars tested. These resistance loci determined in this study should be considered as candidate resistance sources in the development of breeding materials and gene pyramiding studies against rust and common bacterial blight disease of common bean.

Keywords

Common bacterial blight, common bean, resistance resources, molecular marker, rust.

Ülkemizde Yetiştirilen Önemli Fasulye Çeşitlerinin Pas (Uromyces appendiculatus) ve Adi Yaprak Yanıklığı (Xanthomonas axonopodis pv. phaseoli) Hastalıklarına Karşı Dayanıklılık Kaynakları Açısından Değerlendirilmesi

Abstract

Fasulye pası Uromyces appendiculatus ve adi yaprak yanıklığı etmeni Xanthomonas axonopodis pv. phaseoli dünya genelinde fasulye üretim alanlarında yaygın olarak görülen önemli hastalık etmenleridir. Bu hastalık etmenleriyle en etkin ve sürdürülebilir mücadele yöntemi dayanıklı ıslah materyallerinin geliştirilmesidir. Bu amaçla yapılan çalışmalarda söz konusu hastalıklara karşı dayanıklılıkta rol oynayan lokuslar ile ilişkili birçok moleküler markör geliştirilmiş ve ıslah çalışmalarına dâhil edilmiştir. Bu çalışma kapsamında ise ülkemizde yaygın olarak yetiştirilen 40 adet fasulye çeşidi, pas ve adi yaprak yanıklığı hastalıklarına karşı taşıdıkları dayanıklılık kaynakları bakımından SCAR markörleri (SAE19, SI19, SA14, SBC6, SAP6, SU91, BC420) aracılığıyla incelenmiştir. U. appendiculatus’a karşı dayanıklılıkla ilişkili Ur-11 ve Ur-4 lokuslarının çeşitler arasında oldukça yaygın olduğu görülürken, X. axonopodis pv. phaseoli’ye karşı sadece Boncuk, Belinay Sırık, Sırık barbunya, Zülbiye ve Bulduk çeşitlerinin B10 lokusu taşıdığı gözlenmiştir. Çalışma kapsamında tespit edilen bu dayanıklılık lokuslarının fasulye pası ve adi yaprak yanıklığı hastalıklarına karşı ıslah materyallerinin geliştirilmesinde ve gen piramitleme çalışmalarında potansiyel dayanıklılık kaynakları olarak dikkate alınması faydalı olacaktır.

Keywords

Adi yaprak yanıklığı, fasulye, dayanıklılık kaynağı, moleküler markör, pas

References

• Altıkardeşler, A., & Arslan, Ü. (2007). Fasulye (Phaseolus vulgaris L.) çeşitlerinin pas hastalığı (Uromyces appendiculatus (Pers.: Pers.) Unger)’na karşı reaksiyonları ve bazı fungisitlerin etkisi. Journal of Agricultural Faculty, 21(1), 1-8.
• Alzate-Marin, A. L., Souza, T. L. P. O., Ragagnin, V. A., Moreira, M. A., & Barros, E. G. (2004). Allelism tests between the rust resistance gene present in common bean cultivar Ouro Negro and genes Ur-5 and Ur-11. Journal of Phytopathology, 152, 60-64.
• Araya, C. M., Alleyne, A. T., Steadman, J. R., Eskridge, K. M., & Coyne, D. P. (2004). Phenotypic and genotypic characterization of Uromyces appendiculatus from Phaseolus vulgaris in the Americas. Plant Disease, 88(8), 830-836.
• Belete, T., & Bastas, K. K. (2017). Common bacterial blight (Xanthomonas axonopodis pv. phaseoli) of beans with special focus on Ethiopian condition. Journal of Plant Pathology and Microbiology, 8(2), 1000403.
• Broughton, W. J., Hernandez, G., Blair, M., Beebe, S., Gepts, P., & Vanderleyden, J. (2003). Beans (Phaseolus spp.)–model food legumes. Plant and Soil, 252(1), 55-128.
• Carvalho, G. A. D., Paula Junior, T. J., Alzate-Marin, A. L., Nietsche, S., Barros, E. D., & Moreira, M. A. (1998). Herança da resistência da linhagem AND-277 de feijoeiro-comum à raça 63-23 de Phaeoisariopsis griseola e identificação de marcador RAPD ligado ao gene de resistência. Fitopatologia Brasileira, 23, 482-485.
• De Queiroz, V. T., de Sousa, C. S., De Souza, T. L. P. O., Sanglard, D. A., Ragagnin, V. A., de Barros, E. G., & Moreira, M. A. (2004). SCAR marker linked to the common bean rust resistance gene Ur-11. Annual Report.
• Dursun, A., Dönmez, M. F., & Şahin, F. (2002). Identification of resistance to common bacterial blight disease on bean genotypes grown in Turkey. European Journal of Plant Pathology, 108(8), 811-813.
• Edington, B. R., Shanahan, P. E., & Rijkenberg, F. H. J. (1994). Breeding for partial resistance in dry beans (Phaseolus vulgaris) to bean rPha (Uromyces appendiculatus). Annals of Applied Biology, 124, 341-350.
• Faleiro, F. G., Vinhadelli, W. S., Ragagnin, V. A., Corrêa, R., Moreira, M. A., & Barros, E. G. (2000). RAPD markers linked to a block of genes conferring rust resistance to the common bean. Genetics and Molecular Biology, 23, 399-402.
• FAOSTAT. (2020). Food and Agriculture Organization of the United Nations. http://www.fao.org/faostat/en/#data/QC. Erişim tarihi: 23 Aralık 2020.
• Freyre, R., Skroch, P. W., Geffroy, V., Adam-Blondon, A. F., Shirmohamadali, A., Johnson, W. C., Llaca, V., Nodari, R. O., Pereira, P. A., Tsai, S. M., Tohme, J., Dron, M., Nienhuis, J., Vallejos, C. E., & Gepts, P. (1998). Towards an integrated linkage map of common bean. 4.Development of a core linkage map and alignment of RFLP maps. Theoretical and Applied Genetics, 97, 847-856.
• Gillard, C. L., Conner, R. L., Howard, R. J., Pauls, K. P., Shaw, L., & Taran, B. (2009). The performance of dry bean cultivars with and without common bacterial blight resistance in field studies across Canada. Canadian journal of plant science, 89(2), 405-410.
• Grafton, K. F., Weiser, G. C., Littlefield, L. J., & Stavely, J. R. (1985). Inheritance of resistance to two races of leaf rust in dry edible bean. Crop Science, 25, 537-539.
• Haley, S. D., Miklas, P. N., Stavely, J. R., Byrum, J., & Kelly, J. D. (1993). Identification of RAPD markers linked to a major rust resistance gene block in common bean. Theoretical and Applied Genetics, 86(4), 505-512. Hall, R. (1994). Bean diseases, bean pathogens, bean disease control. Compendium of Bean Diseases, APS press, Minnesota, USA.
• Johnson, E., Miklas, P. N., Stavely, J. R., & Martinez-Cruzado, J. C. (1995). Coupling-and repulsion-phase RAPDs for marker-assisted selection of PI 181996 rust resistance in common bean. Theoretical and Applied Genetics, 90(5), 659-664.
• Jung, G., Coyne, D. P., Skroch, P., Nienhuis, J., Arnaud-Santana, E., Bokosi, J., Ariyarathne, H., Steadman, J., Beaver, J., & Kaeppler, S. (1996). Molecular markers associated with plant architecture and resistance to common blight, web blight, and rust in common beans. Journal of the American Society for Horticultural Science, 121, 794-803.
• Kelly J. D., Gepts, P., Miklas, P. N., & Coyne, D. P. (2003). Tagging and mapping of genes and QTL and molecular-marker assisted selection for traits of economic importance in bean and cowpea. Field Crops Research, 82, 135-154.
• Liebenberg, M. M., Mienie, C. M. S., & Pretorius, A. Z. (2006). The occurrence of rust resistance gene Ur-13 in common bean cultivars and lines. Euphytica, 150, 365-386. Lindgren, D. T., Eskridgre, K. M., Steadman, J. R., & Schaaf, D. M. (1995). A model for dry bean yield loss due to rust. HortTechhnology, 5, 35-37.
• McClean, P., & Myers, J. (1990). Pedigrees of dry bean cultivars, lines and PIs. Annual Report of the Bean Improvement Cooperative, 33, 25-30.
• Mienie, C. M. S., Naidoo, R., & Liebenberg, M. M. (2004). Conversion of the RAPD Marker for Ur-4 to a Co-Dominant SCAR Marker SA 14~ 1~ 0~ 7~ 9~/~ 8~ 0~ 0. Annual Report-Bean Improvement Cooperatıve, 47, 261-262.
• Miklas, P. N., Kelly, J. D., Beebe, S. E., & Blair, M. W. (2006). Common bean breeding for resistance against biotic and abiotic stresses:from classical to MAS breeding. Euphytica, 147, 105-131.
• Miklas, P. N., Pastor-Corrales, M. A., Jung, G., Coyne, D. P., Kelly, J. D., McClean, P. E., & Gepts, P. (2002). Comprehensive linkage map of bean rust resistance genes. Annual Report of the Bean Improvement Cooperative, 45, 125-129.
• Miklas, P. N., Stone, V., Daly, M. J., Stavely, J. R., Steadman, J. R., Bassett, M. J., Delorme, R., & Beaver, J. S. (2000). Bacterial, fungal, and viral disease resistance loci mapped in a recombinant inbred common bean population (Dorado/XAN 176). Journal of the American Society for Horticultural Science, 125, 476-481.
• Mutlu, N., Miklas, P., Reiser, J., & Coyne, D. (2005). Backcross breeding for improved resistance to common bacterial blight in pinto bean (Phaseolus vulgaris L.). Plant Breeding, 124(3), 282-287.
• Park, S. O., Coyne, D. P., Steadman, J. R., Crosby, K. M., & Brick, M. A. (2004). RAPD and SCAR markers linked to the Ur‐6 Andean gene controlling specific rust resistance in common bean. Crop Science, 44(5), 1799-1807.
• Pastor-Corrales, M. A., Pereira, A. P. A., Lewers, K., Brondani, R. V., Buso, G. C., Ferreira, M. A., & Martins, W. S. (2008). Identification of SSR markers linked to rust resistance in Andean common bean PI 260418. Annual Report of the Bean Improvement Cooperative, 51, 46-47.
• Pastor-Corrales, M. A. (2005). Inheritance of resistance in PI260418 an Andean bean resistant to most races of the bean rust pathogen. Annual Report of the Bean Improvement Cooperative, 48, 134-135.
• Pedraza, F., Gallego, G., Beebe, S., & Tohme, J. (1997). Marcadores SCAR y RAPD para la resistencia a la bacteriosis comun (CBB). Taller de mejoramiento de frijol para el Siglo XXI: Bases para una estrategia para America Latina, 130-134.
• Poyraz, İ., Şahin, B., & Atmaca, E. (2017). Detection of Ten Resistance Genes Against P. syringae pv. phaseolicola and X. axonopodis pv. phaseoli in Twelve Local Bean Varieties Using SCAR Markers. Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 7(2), 241-248.
• Rasmussen, J. B., Grafton, K. F., Gross, P. L., & Donohue, C. M. (2002). Genetics of rust resistance in Compuesto Negro Chilmaltenango (CNC). Annual Report of the Bean Improvement Cooperative, 45, 94-95.
• Sartorato, A., Nietsche, S., Barros, E. G, & Moreira, M. A. (1999). Inheritance of angular leaf spot resistance and RAPD markers linked to disease resistance gene in common beans. Annual Report of the Bean Improvement Cooperative, 42, 21–22.
• Souza, T. L. P. O., Dessaune, S. N., Sanglard, D. A., Moreira, M. A., & Barros, E. G. (2007a). Rust resistance gene present in common bean cultivar Ouro Negro (Ur-ON) does not correspond to Ur-3+. Annual Report of the Bean Improvement Cooperative, 50, 119-120.
• Souza, T. L. P. O., Alzate-Marin, A. L., Dessaune, S. N., Nunes, E. S., Queiroz, V. T., Moreira, M. A., & Barros, E. G. (2007b). Inheritance study and validation of SCAR molecular marker for rust resistance in common bean. Crop Breeding and Applied Biotechnology, 7, 11-15.
• Souza, T. L. P. O., Alzate-Marin, A. L., Faleiro, F. G., & Barros, E. G. (2008). Pathosystem common bean-Uromyces appendiculatus: host resistance, pathogen specialization, and breeding for rust resistance. Pest Technology, 2, 56-69.
• Souza, T. L. P., Faleiro, F. G., Dessaune, S. N., Paula-Junior, T. J. D., Moreira, M. A., & Barros, E. G. D. (2013). Breeding for common bean (Phaseolus vulgaris L.) rust resistance in Brazil. Tropical Plant Pathology, 38(5), 361-374.
• Stavely, J. R., & Grafton, K. F. (1985). Genetics of resistance to eight races of Uromyces appendiculatus in Phaseolus vulgaris cultivar Mexico 235. Phytopathology, 75, 1310.
• Tugume, J. K., Tusiime, G., Sekamate, A. M., Buruchara, R., & Mukankusi, C. M. (2019). Diversity and interaction of common bacterial blight disease-causing bacteria (Xanthomonas spp.) with Phaseolus vulgaris L. The Crop Journal, 7(1), 1-7.
• TÜİK. (2019). Türkiye İstatistik Kurumu, Bitkisel Üretim İstatistikleri. http://www.tuik.gov.tr. Erişim tarihi 20 Aralık 2020.
• Yeken, M. Z., Özer, G., Çelik, A., & Çiftçi, V. (2019). Tescilli Fasulye Çeşitlerinin Pas (Uromyces appendiculatus) Etmenine Karşı Dayanıklılık Durumlarının SCAR Markörleri ile Belirlenmesi. Türk Tarım ve Doğa Bilimleri Dergisi, 6(3), 410-416.
• Yeken, M. Z., Kantar, F., Çancı, H., Özer, G., & Çiftçi, V. (2018). Breeding of dry bean cultivars using Phaseolus vulgaris landraces in Turkey. International Journal of Agricultural and Wildlife Sciences, 4(1), 45-54.
• Yu, K., Park, S. J., & Poysa, V. (2000). Marker-assisted selection of common beans for resistance to common bacterial blight: efficiency and economics. Plant Breeding, 119, 411-416.
• Zaiter, H. Z., Coyne, D. P., & Steadman, J. R. (1989). Inheritance of resistance to a rust isolate in beans. Annual Report of the Bean Improvement Cooperative, 32, 126-127.