Comparison of thewound healing effects of vascular endothelial growth factor a (VEGF-A) and transforming growth factor beta 3 (TGF-β3) on gingival cells

Year 2023, Volume: 7 Issue: 2, 151 - 156, 31.12.2023

Serkan Yelli , Aslı Yıldırım , Seçil Erden Tayhan , Sema Bilgin , İsa Gökçe

https://doi.org/10.32571/ijct.1249472

Abstract

Each tissue has its own anatomy and physiology. The deterioration of these structures by various effects (crushing, puncture, pathogens, etc.) is called as wound. The tissue exposed to these effects wants to gain the same function and anatomy. This phenomenon is called wound healing. Wound healing is a process that involves many reactions and cytokines. Since it is known that TGF-β3 and VEGF-A proteins which are among these cytokines, play an active role in wound healing stages. In the present study, it was decided to examine the wound healing effects of the non human gingival fibroblast cells comperatively. Wound healing process under the influence of these cytokines was performed by in vitro scratch analysis which is known as easy, reliable and cheap technique. In this context, firstly cell viability analysis was performed to determine the most effective dose for inducing cell proliferation. After this experimental step, gingival cells were incubated for 72 hours with suitable TGF-β3 and VEGF-A doses. As a result of the study, it was concluded that TGF-β3 and VEGF-A proteins are not cytotoxic and have a wound-healing effect on gingival fibroblast cells.

Keywords

TGF-β3, VEGF-A, Gingival fibroblast cells, wound healing.

Supporting Institution

Tokat Gaziosmanpaşa BAP Birimi

Project Number

2021/27

Thanks

The study is part of Serkan Yelli’s doctoral thesis that financially supported by Tokat Gaziosmanpaşa University Scientific Research Projects (2021/27).

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