ULTRASTRUCTURAL EXAMINATIONS ON DIABETIC RAT SKIN TISSUE WITH TOPICAL APPLICATION OF Salvia euphratica ETHANOL EXTRACT

Year 2018, Volume: 3 Issue: 3, 107 - 116, 26.12.2018

Ebru Gokalp-ozkorkmaz , Nursel Gul , Yusuf Ozay Sevda Guzel Ahmet Kahraman Hakan Eskızengın

https://doi.org/10.23884/ijhsrp.2018.3.3.02

Cited By: 2

Abstract

Several medicinal plants to treat diabetic wound
healing were researched and are still under investigation. A variety of processes
contributed to diabetics such as; inhibition of inflammatory response, generation of reactive oxygen species, differentiation of the extracellular matrix
and reduced collagen production. The purpose of this study was to examine the ultrastructural
alterations in diabetic rat skin tissue treated with the ointment prepared with Salvia euphratica. Male Wistar albino rats were used in
this study (n: 24), divided into 4 groups. Non-treated, diabetic, diabetic and
cicatrizant  treated, ointment prepared
with ethanol extract of   1% Salvia
euphratica  topically applied  for  14
days. A single dose of 45 mg/dL streptozotocin (i.p.) was given to rats to
induce diabetes. Excisional wound model was created under anesthesia. A cicatrizant,
fitocream, was used as positive control. Normal skin tissue was observed in
non-treated group. Diabetic skin tissue  
revealed hyalinization of the cytoplasm and loss of cytoplasm.    Ointment treated diabetic group revealed
altered cellular elements suggests healing. At the wound area, number of
fibroblast cells synthesizing connective tissue were increased and the collagen
fibers were regularly oriented. Neutrophil and monocyte cells besides mast and
macrophage cells were found in the capillaries of dermis in S. euphratica
ointment treated group. Wound healing in diabetic tissues is known to
be slow. Using phytocream therapy to expedite the healing process is a long
time known phenomenon. Our observations demonstrated that Salvia euphratica
promoted changes in skin tissue that may contribute to wound healing at the
cellular level.

Keywords

Diabetes, Salvia euphratica, Wound Healing, Ultrastructure

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