HISTOLOGICAL AND HISTOCHEMICAL EVALUATION OF NORMOTENSIVE AND PREECLAMPTIC PLACENTAS

Year 2019, Volume: 4 Issue: 1, 67 - 73, 01.04.2019

Gamze Erdogan , Yusuf Nergiz , Elif Ağaçayak

https://doi.org/10.23884/ijhsrp.2019.4.1.07

Abstract

The placenta plays a role in the pathophysiology of preeclampsia.
Preeclampsia is more common in multifetal pregnancies than singleton
pregnancies. In this study, we aimed to investigate the histopathology of
normotensive and preeclamptic placentas and the localization of alkaline
phosphatase activity. In our study, 10 normotensive and 10 preeclamptic,
totally 20 placentas were obtained.Paraffin sections were stained with
Hematoxylin-Eosin, Masson trichrome and PAS for histopathological
examination.Remaining sections were then stained via Gomori’s method and
micrographed under light microscope. Sections of the control group observed
normal histologically structure.The 
alkaline phosphatase reaction was evident in the inner and outer
membranes of the syncytiotrophoblasts. There was a significant increase in the
number of syncytial knots, terminal villi and syncytial bridges in the
preeclamptic placenta sections. Marked thickening of the trophoblast basal
membranes were observed. Alkaline phosphatase reaction in preeclampsia
group: The localization of alkaline phosphatase in the inner and outer
membranes of the syncytiotrophoblasts was reduced. In placentas of preeclampsia
group, a significant increase in syncytial knot, syncytial bridge were
observed. The level of alkaline phosphatase enzyme in preeclamptic placentas
was found to be lower compared to normotensive placenta.

Keywords

Placenta, Preeclampsia, Alkaline phosphatase, Light microscope

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