Isolation of bacteria from Tuz Gölü lake that can grow on high salt concentration

Year 2019, Volume: 2 Issue: 3, 158 - 164, 28.12.2019

Jyng Sheng Tee , Yilmaz Kaya , Fahrul Zaman Huyop

https://doi.org/10.38001/ijlsb.581131

Cited By: 2

Abstract

Today, extremophile is widely studied by the scientist
due to its strong survival features that allow them to survive under extreme
environment. Halophile is one example that inhabit high salt concentration
environment. Isolation of bacteria from the area of Tuz Gölü lake, also known
as hypersaline lake in the central plateau of Turkey, led to the isolation of 4
halotolerant bacteria, which were able to grow optimally in media with 0–10% of
salt. Surprisingly, the strain A-4 isolate was successfully isolated from the
Tuz Gölü lake water on the minimal media that consists of 2,2-dichloropropionic
acid as a carbon source. This indicated that the strain A-4 was very useful in
the environmental remediation due to its capability to break down 2,2-DCP, a halocarboxylic found in herbicide.  Further analysis such as biochemical tests
and 16srRNA sequence analysis were necessary to
further identify the species of the bacteria in the next experiment.

Keywords

Halophile, halotolerant bacteria, tuz gölu

Supporting Institution

Universiti Teknologi Malaysia

Thanks

This work was financially supported by Biosciences Department, Universiti Teknologi Malaysia for internal grants for undergraduate research. Authors also thank TUBITAK for financial awards for sampling.

References

• Camur, M. Z., and Mutlu, H., Major-ion geochemistry and mineralogy of the Salt Lake (Tuz Gölü) basin, Turkey. Chemical Geology, 1996.127(4): p. 313-329.
• Dumorné, K., Córdova, D. C., Astorga-Eló, M., and Renganathan, P., Extremozymes: a potential source for industrial applications. Journal Microbiol Biotechnol, 2017. 27(4): p.649-659.
• Edbeib, M. F., Wahab, R. A., Kaya, Y., and Huyop, F., In silico characterization of a novel dehalogenase (DehHX) from the halophile Pseudomonas halophila HX isolated from Tuz Gölü Lake, Turkey: insights into a hypersaline-adapted dehalogenase. Annals of Microbiology, 2017. 67(5): p. 371-382.
• Hareland, W. A., Crawford, R. L., Chapman, P. J., and Dagley, S., Metabolic function and properties of 4-hydroxyphenylacetic acid 1-hydroxylase from Pseudomonas acidovorans. Journal of Bacteriology, 1975. 121(1), 272-285.
• Huston, A. L., Krieger-Brockett, B. B., & Deming, J. W., Remarkably low temperature optima for extracellular enzyme activity from Arctic bacteria and sea ice. Environmental microbiology, 2000. 2(4), 383-388.
• Ma, Y., Galinski, E. A., Grant, W. D., Oren, A., and Ventosa, A., Halophiles 2010: life in saline environments. In: Am Soc Microbiol., 2010.
• Mir, S. I., Gasim, M. B., Rahim, S. A., and Toriman, M. E., Soil loss assessment in the Tasik Chini catchment, Pahang, Malaysia., 2010.
• Mutlu, M. B., Martínez-García, M., Santos, F., Peña, A., Guven, K., & Antón, J., Prokaryotic diversity in Tuz Lake, a hypersaline environment in Inland Turkey. FEMS microbiology ecology, 2008. 65(3), 474-483
• Ollivier, B., Caumette, P., Garcia, J.-L., & Mah, R., Anaerobic bacteria from hypersaline environments. Microbiology and Molecular Biology Reviews, 1994. 58(1), 27-38.
• Paul, V. G., & Mormile, M. R., A case for the protection of saline and hypersaline environments: a microbiological perspective. FEMS microbiology ecology,2017. 93(8).
• Sorokin, D. Y., Tourova, T., Galinski, E., Belloch, C., & Tindall, B., Extremely halophilic denitrifying bacteria from hypersaline inland lakes, Halovibrio denitrificans sp. nov. and Halospina denitrificans gen. nov., sp. nov., and evidence that the genus name Halovibrio Fendrich 1989 with the type species Halovibrio variabilis should be associated with DSM 3050. International journal of systematic and evolutionary microbiology, 2006.56(2), 379-388.