Characterization of Omega-3 and Omega-6 Fatty Acid Accumulation in Chlorococcum novae-angliae Microalgae Grown under Various Culture Conditions

Year 2022, Volume: 5 Issue: 3, 346 - 369, 15.12.2022

Elifcan Çalışkan Berat Zeki Haznedaroğlu

https://doi.org/10.38001/ijlsb.1082112

Cited By: 4

Abstract

Chlorococcum novae-angliae is a terrestrial green microalgae species with remarkable potential to synthesize omega-3 (ω-3) and omega-6 (ω-6) fatty acids. In this study, Chlorococcum novae-angliae has been subjected to varying growth conditions (light, nitrogen, salinity, and temperature) to investigate the accumulation of ω-3 and ω-6 fatty acids. Among tested growth conditions, eicosapentaenoic acid, α-linoleic acid, γ-linoleic acid, and arachidonic acid were enhanced by nitrogen limitation. Significant increases were observed in concentration of linoleic acid, an essential precursor molecule for the production ω-6 fatty acids under decreased nitrogen concentrations. Despite the lowest biomass growth, monounsaturated fatty acids and docosahexaenoic acid were increased by 14.4% and 8.7% under low light intensities, respectively. Meanwhile, the highest concentrations of palmitic acid (C16:0), stearic acid (C18:0), and oleic acid (18:1cis-9) were also detected under nitrogen limitation. Total lowest fatty acid concentrations were obtained under increased salinity while low temperature conditions heavily inhibited cellular growth.

Keywords

Chlorococcum novae-angliae, essential fatty acids, microalgal lipids, omega fatty acids

Supporting Institution

TÜBİTAK

Project Number

2180589

Thanks

This work was financially supported by The Scientific and Technological Research Council of Turkey (TÜBİTAK) 1512 Program (Award No: 2180589). Infrastructure used in this study was supported through Turkish Ministry of Industry and Technology & Directorate General for EU and Foreign Affairs Department of EU Financial Programmes, Competitiveness and Innovation Sector Operational Programme (Award No: EuropeAid/140111/IH/SUP/TR). E.C. was supported by Istanbul Development Agency (Award No: TR10/15/YNK/0062), B.Z.H. was supported by Royal Society Newton Fellowship (Award No: NA140481). We also would like to acknowledge Asst. Prof. Dr. Mehmet Firat Ilker (Department of Chemistry, Bogazici University) for providing access to GC instrument.

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