Morinda reticulata Gamble Tubers: A Potent Hepatoprotective Agent Against CCl4 and Paracetamol Induced Hepatotoxicity in Wistar Albino Rats

Year 2020, Volume: 4 Issue: 1, 26 - 39, 01.06.2020

Raju Asirvatham , Joshila Akhil

Abstract

Liver toxicityis the common issue in social life and it may induce by various chemicals and drugs. The hepatoprotective study was aimed to explore the extracts of Morinda reticulata Gamble for hepatoprotective activity against CCl4 and paracetamol induced hepatotoxicity on Wistar albino rats. Treatment group rats (4 groups, n=6 in each) were received either 200 mg/kg and 400 mg/kg of ethanol or aqueous extract of M. reticulata (p.o) and one group (n=6) was received 25 mg/kg of silymarin (p.o) twice daily for 7 days. CCl4 (1.5 ml/kg body weight, p.o) or paracetamol (2 g/kg) intoxication was done after the last dose of treatment and standard drug administration. Hepatoprotective potential was determined on the basis of measurement of hepatic serum marker enzymes such as Alanine Transaminase (AST), Aspartate transaminase (AST), Alkaline Phosphatase (ALP), Total bilirubin and liver weight. Further, liver tissue was subjected to histopathological studies. HPTLC studies were done to determine the presence of phytochemical constituents. Pretreatment with silymarin, ethanol and aqueous tuber extract of M. reticulata significantly (p < 0.001) reduced the CCl4 or paracetamol induced elevated AST, ALT, ALP, Total bilirubin level in serum and also reduced liver weight and total protein levels. HPTLC study of ethanol extract showed six distinct peaks for chemical constituents. The present study indicated that ethanol and aqueous tuber extract of M. reticulata has potential hepatoprotective effect against chemicals and it can be used as a clinical remedy for hepatotoxicity disorders.

Keywords

Hepatoprotective, HPTLC fingerprint, Morinda reticulata Gamble, paracetamol, sylimarin

Supporting Institution

SKCPRC, Parassala

Project Number

PG/005

Thanks

The authors gratefully acknowledge Dr. Sr. Betty Carla, Director and Dr. Vinod B, Principal, St. Joseph’s College of Pharmacy for providing support and facilities for this research work.

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