Determination of Antioxidant Activities in Raw and Boiled Extractions of Raphanus raphanistrum L. Species Naturally Growing in Edremit Gulf (Balıkesir/Turkey)

Year 2023, Volume: 7 Issue: 2, 65 - 78, 30.12.2023

Hatice İnci Aladı , Tülin Aşkun , Selami Selvi

https://doi.org/10.47947/ijnls.1338186

Cited By: 1

Abstract

The plants that naturally spread in Edremit Gulf are consumed by the local people in various ways either cooked or raw. In this study, Raphanus raphanistrum species from the Brassicaceae family, which is also known to be used as food, was used. DPPH and CUPRAC antioxidant activity analyses were carried out on the extracts obtained from the aerial parts of the raw and boiled fresh plant. The total phenolic contents were determined and the phenolic compounds contained and their interactions with the boiling process were determined by HPLC method. The extracts were stored in a deep freezer at -20 °C to be used in further studies. In terms of the total amount of phenolic compounds, 12.65 µg GAE/mg extract was detected in the boiled extract of R. raphanistrum species, and it has a higher value compared to the raw extract. In the boiled extract, which has a higher value in terms of total flavonoid content than raw, was found 54 µg Rutin Equivalent /mg fresh extract. Compared to the CUPRAC method, the boiled extract showed higher (68.58 µgTE/mg extract) activity. The antioxidant activity of the boiled extract was found to be higher than the DPPH method, and the EC 50 value was measured as 1975.25 µg/mL. According to HPLC method, resveratrol, rosmarinic acid, quersetin, kaempferol and naringenin substances were determined as in the raw extract. It was determined that while naringenin was destroyed in the boiled extract, were changed the amounts of other phenolic compounds.

Keywords

Antioxidant activity, Edremit gulf, flavonoid, phenolic, raw and boiled extract

References

• Aladı, H. İ., Satıl, F., & Selvi, S. (2022). Yenilebilir doğal bitkilerin etnobotanik ve gastronomik açıdan değerlendirilmesi: Edremit Körfezi (Balıkesir) örneği . Journal of the Institute of Science and Technology, 12 (3), 1375-1385. https://doi.org/10.21597/jist.1085847
• Apak, R., Güçlü, K., Özyürek, M., & Karademir, S. E. (2004). Novel antioxidant capacity index for dietary polyphenols and vitamin C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method. Journal of Agricultural and Food Chemistry, 52, 7970-7981.
• Apak, R., Güçlü, K., Özyürek, M., Karademir, S. E., & Erçag, E. (2006). The cupric ion reducing antioxidant capacity and polyphenolic content of some herbal teas. International Journal of Food Sciences and Nutrition, 57, 292-304.
• Battin, E. E., & Brumaghim, J. L. (2009). Antioxidant activity of sulfur and selenium: A review of reactive oxygen species scavenging, glutathione peroxidases, and metal-binding antioxidant mechanisms. Cell Biochemistry and Biophysics, 55, 1-23.
• Baytop, T. (1999). Türkiye’ de Bitkiler ile Tedavi (ilaveli ikinci baskı). İstanbul: Nobel Tıp Kitabevi, 193–194.
• Benzie, I. F. F. (2000). Evaluation of antioxidant defense mechanisms. European Journal of Nutrition, 39, 53-61.
• Bolkent, S., Yanardağ, R., & Karabulut-Bulan, O. (2001). The effects of Melissa officinalis on liver of hyperlipidaemic rats: A morphological and biochemical study. Proceedings 10th Scientific Conference Electron Microscopy Society of Malaysia, 8-10th November, Kuala Lumpur.
• Calligaris, S., Manzocco, L., Anese, M., & Nicoli, M.C. (2004). Effect of heat treatment on the antioxidant and pro-oxidant activity of milk. International Dairy Journal, 14, 421-427.
• Caponio, F., Alloggio, V., & Gomes T. (1999): Phenolic compounds of virgin olive oil: Influence of paste preparation techniques, Food Chemistry, 64, 203–204.
• Davis, P. H (Ed.). (1965). Flora of Turkey and the East Aegean Islands (Vol. 7). Edinburgh UK.: Edinburgh University Press.
• Güner, A., Aslan, S., Ekim, T., Vural, M., & Babaç, M. T. (2012). Türkiye Bitkileri Listesi (Damarlı Bitkiler). İstanbul: Nezahat Gökyiğit Botanik Bahçesi ve Flora Araştırmaları Derneği Yayını.
• Halvorsen B. L., Holte K., Myhrstad M. C. W., Barikmo I., Hvattum E., Remberg S. F., Wold, A. B., Haffner, K., Baugerod, H., Andersen, L. F., Moskaug, Q., Jacobs, D. R., & Blomhoff, R. (2002). “A systematic screening of total antioxidants in dietary plants. The Journal of Nutrition, 132 (3), 461-471.
• Huang, D., Ou, B., & Prior, R. L. (2005). The chemistry behind antioxidant capacity assays. Journal of Agricultural and Food Chemistry, 53 (6), 1841-1856.
• Kim, S. Y., Jeong, S. M., Park, W. P., Nam, K. C., Ahna, D. U., & Lee, S. C. (2006). Effect of heating conditions of grape seeds on the antioxidant activity of grape seed extract. Food Chemistry, 97, 472-479.
• Kopustinskiene, D. M., Jakstas, V., Savickas, A., & Bernatoniene, J. (2020). Flavonoids as anticancer agents. Nutrients, 12 (2), 457. https://doi.org/10.3390/nu12020457.
• Kumar, S., & Pandey, A. K. (2013). Chemistry and biological activities of flavonoids: an overview. The Scientific World Journal, 162750. https://doi.org/10.1155/2013/162750.
• Kumaran, A., & Karunakaran, J.R. (2007). In vitro antioxidant activities of methanol extracts of five Phyllanthus species from India. Food Science and Technology, 40, 344–352.
• Mazzeo, T., N Dri, D., Chiavaro, E., Visconti, A., Fogliano, V. (2011). Effect of two cooking procedures on phytochemical compounds, total antioxidant capacity and colour of selected frozen vegetables. Food Chemistry, 128, 627-633.
• Oboh, G. (2005). Effect of blanching on the antioxidant properties of some tropical green leafy vegetables. LWT - Food Science and Technology, 38 (5), 513–517.
• Onar-Celik, H., Yusufoğlu, A., Türker, G., & Yanardağ, R. (2012). Elastase, tyrosinase and lipoxgenase inhibition and antioxidant activity of an aqueous extract from Epilobium angustifolium L. leaves. Journal of Medicinal Plants Research, 6, 716-726.
• Pinelo, M., Rubilar, M., Sinerio, J., & Nunez, M.J. (2005). A thermal treatment to increase the antioxidant capacity of natural phenols: catechin, resveratrol and grape extract cases. European Food Research and Technology, 221, 284-290.
• Polat, R., & Selvi, S. (2011). Edremit Körfezi’nin (Balıkesir) arı bitkileri üzerine bir araştırma. Harran Üniversitesi Ziraat Fakültesi Dergisi, 15(2), 27-32.
• Roginsky A. B., Ujiki M.B, Ding X. Z., & Adrian T. E. (2005). On the potential use of flavonoids in the treatment and prevention of pancreatic cancer. In Vivo, 219, 61-67.
• Sakac, M., Torbica, A., Sedej, I., & Hadnadev, M. (2011). Influence of breadmaking on antioxidant capacity of gluten free breads based on rice and buckwheat flours. Food Research International, 44, 2806- 2813.
• Shi, S., Li, J., Zhao, X., Liu, Q., & Song, S. J. (2021). A comprehensive review: Biological activity, modification and synthetic methodologies of prenylated flavonoids. Phytochemistry, 191, 112895. https://doi.org/10.1016/ j.phytochem.2021.112895
• Slinkard, K., & Singleton, V. L. (1977). Total phenol analyses: automation and comparison with manual methods. American Journal of Enology and Viticulture, 28, 49-55.
• The Plant List (2010). Version 1. Published on the Internet; http://www.theplantlist.org/ (accessed 1 September 2023). Turkmen, N., Sari, F., & Velioglu, Y.S. (2005). The effect of cooking methods on total phenolics and antioxidant activity of selected green vegetables. Food Chemistry, 93, 713-718.
• Volden, J., Borge, G. I. A., Bengtsson, G. B., Hansen, M., Thygesen, I. E., & Wicklund, T. (2008). Effect of thermal treatment on glucosinolates and antioxidant- related parameters in red cabbage (Brassica oleracea L. ssp capitata f. rubra). Food Chemistry, 109, 595–605.
• Yıldırım, E., Dursun, A., & Turan, M. (2001). Determination of the nutrition contents of the wild plants used as vegetables in upper Çoruh Valley, Turkish Journal of Botany, 25, 367-371.
• Zhishen, J., Mengcheng, T., & Jianming, W. (1999). The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry, 64, 555-559.