Comparison of the Aflatoxin levels in Fish Feeds from Rainbow Trout Farms Localized at Different Regions (Adana-Ağrı, TURKEY)

Year 2019, Volume: 5 Issue: 2, 78 - 84, 31.12.2019

İbrahim Cengizler , Selvinaz Yakan , Selçuk Duman , Miray Etyemez Büyükdeveci , Ruhay Aldık

https://doi.org/10.29132/ijpas.522652

Abstract

Aflatoxins levels in different fish feeds
obtained from two different rainbow trout (Oncorhynchus
mykiss) farms located in Adana and Ağrı regions were determined between May
and July 2017. During the experiment period, ''a drought status'' was reported
according to the Standardized Precipitation Index of Turkey by Turkish State
Meteorological Service. According to precipitation index records, while Adana
was reported as slightly humid, Ağrı was slightly dry in the period between May
and July 2017. The aflatoxin analyses of fish feed samples was carried out in
the laboratories of the Ministry of Food, Agriculture and Livestock in Adana,
Turkey. The aflatoxin levels of the samples were resolved by liquid-solid
extraction, immune-affinity pillar clean-up using a HPLC-FD (High Performance
Liquid Chromatography with Fluorescence Detection). The detection limits of
aflatoxin were 0.50, 0.23, 0.73, 0.20 ppb for AFB1, AFB2, AFG1, AFG2,
respectively. The results showed that, aflatoxin levels of fish feeds were
below the detection limits in all samples.Aflatoxins levels in different fish feeds
obtained from two different rainbow trout (Oncorhynchus
mykiss) farms located in Adana and Ağrı regions were determined between May
and July 2017. During the experiment period, ''a drought status'' was reported
according to the Standardized Precipitation Index of Turkey by Turkish State
Meteorological Service. According to precipitation index records, while Adana
was reported as slightly humid, Ağrı was slightly dry in the period between May
and July 2017. The aflatoxin analyses of fish feed samples was carried out in
the laboratories of the Ministry of Food, Agriculture and Livestock in Adana,
Turkey. The aflatoxin levels of the samples were resolved by liquid-solid
extraction, immune-affinity pillar clean-up using a HPLC-FD (High Performance
Liquid Chromatography with Fluorescence Detection). The detection limits of
aflatoxin were 0.50, 0.23, 0.73, 0.20 ppb for AFB1, AFB2, AFG1, AFG2,
respectively. The results showed that, aflatoxin levels of fish feeds were
below the detection limits in all samples. 

 

Keywords

Aflatoxin, Fish feed, HPLC analysis, Rainbow trout farm

Farklı Bölgelerde (Adana-Ağrı, TÜRKİYE) Bulunan Gökkuşağı Alabalığı Çiftliklerindeki Balık Yemlerinde Aflatoksin Düzeylerinin Karşılaştırılması

Abstract

Mayıs ve Temmuz 2017 tarihleri arasında
Adana ve Ağrı bölgelerinde bulunan iki farklı gökkuşağı alabalığı (Oncorhynchus mykiss) çiftliğinden elde
edilen farklı balık yemlerinde aflatoksin seviyeleri belirlendi. Çalışmamıza
ait dönemde Türkiye aylık standart yağış indeksine göre kuraklık durumu
kaydedilmiştir. Kayıtlarımıza göre Ağrı ili Mayıs-Temmuz aylarında hafif kurak,
Adana ili ise hafif nemli bir hava göstermiştir. Yem örneklerinin analizi T.C.
Adana Gıda Tarım ve Hayvancılık Laboratuvarlarında yürütülmüştür. Numuneler
floresan saptama (HPLC-FD) ile yüksek performanslı sıvı kromatografisi
kullanılarak, sıvı-katı özütleme immune-affinity column clean-up metodu ile
belirlenmiştir. Aflotoksin tespit limiti AFB1, AFB2, AFG1, AFG2 için sırasıyla
0,50; 0,23; 0,73; 0,20 ppb, olarak belirlenmiştir. Örneklenen tüm balık
yemlerinde aflatoksin düzeylerin tespit edilebilir değerin altında olduğu
bulunmuştur.

Keywords

Aflatoksin, balık yemi, HPLC analizi, gökkuşağı alabalığı çiftliği

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