An easy and reliable method for establishment and maintenance of tissue cultures of Nicotiana tabacum cv TAPM 26

Year 2020, Volume: 2 Issue: 2, 62 - 71, 26.08.2020

Yilmaz Kaya , Fahrul Zaman Huyop

https://doi.org/10.38058/ijsl.764947

Cited By: 5

Abstract

In vitro regeneration of Nicotiana tabacum was efficiently achieved using growth regulators combination supplemented into semi-solid MS medium using leaves from 1-month-old plant as explants. The growth regulators used were NAA for auxin (0.0, 1.0, 2.0, and 3.0 mgL^-1) and BAP (0.0, 0.5, 1.0, 2.5, and 5.0 mgL^-1). The effects of growth regulator combination were assessed based on a number of callus formation, shoots formation and fresh callus weight. The maximum number of callus formation was 100 % at five hormone combination, observed at the fourth week after culture. Maximum number of shoots produced per explant was 21.4 shoots at 1.0 mgL^-1 BAP + 3.0 mgL^-1 NAA, after 6 weeks of culture. The maximum callus fresh weights were obtained at 0.5 mgL^-1 BAP + 1.0 mgL^-1 NAA after 6 weeks (9.92 g). The best combination for shoots regeneration of Nicotiana tabacum was 1.0 mgL^-1 BAP + 3.0 mgL^-1 NAA. And the best combination for heaviest callus production was 0.5 mgL^-1 BAP + 1.0 mgL^-1 NAA.

Keywords

Nicotiana tabacum TAPM 26, Callus,, tissue culture

References

• 1. Ali,G., Hadi,F., Ali,Z., Tariq,M. and Khan,M.A.(2007). Callus induction and in vitro complete plant regeneration of different cultivars of tobacco (Nicotiana tobacum L.) on media of different hormonal concentration. Journal of Biotechnology Vol.6 (4), 561-566
• 2. Andrey S. Marchev and Milen I. Georgiev(2020). Plant In Vitro Systems as a Sustainable Source of Active Ingredients for Cosmeceutical Application. Molecules , 25, 2006; doi:10.3390/molecules25082006
• 3. Arvas, Y.E., H.M. Aksoy, and Y. Kaya, Patates Bitkisinde Biyoteknolojik Çalışmalar. International Journal of Life Sciences and Biotechnology, 2018. 1(1): p. 37-47.
• 4. Deo, P.C., Tyagi, A.P., Taylor, M., Harding, R. And Becker, D. 2010. Factors affecting somatic embryogenesis and transformation in modern plant breeding. South Pac. J. Nat. App. Sci, 28:27–40.
• 5. Ganasan K. and Fahrul Huyop (2010) In vitro regeneration of Citrullus lanatus cv. Round Dragon. Journal of Biological Sciences. 10(2):131-137. ISSN 1727-3048.
• 6. Ganapathi TR, Suprasanna P, Rao PS, Bapat VA. Tobacco (Nicotiana tabacum L.) - A model system for tissue culture interventions and genetic engineering. Indian J Biotechnol 2004;3:171-84.
• 7. Jawad, Z.A., Turker, M. and Ozdemir, F.A. 2020. Effect of different plant growth regulator on in vitro propagation of endangered plant; yellow tomato (Lycopersicon esculentum Mill.). Int. J. Agric. For. Life Sci., 4(1): 92-98.
• 8. Kaya, Y., in vitro Plant Regeneration of Tobacco. , in Faculty of Biosciences and Bioengineering. 2010, Universiti Teknologi Malaysia: Malaysia.
• 9. Kaya Y, S. Yilmaz, S. Marakli, N. Gozukirmizi and F. Huyop, “Transformation of Nicotiana tabacum with DehE Gene,” Journal of Food Agriculture and Environment, Vol. 11, No. 3-4, 2013
• 10. Kaya, Y. and S. Karakütük, Farklı büyüme düzenleyicilerin Türk kır çeltiği rejenerasyonuna etkisi. Anadolu Tarım Bilimleri Dergisi, 2018. 33(3): p. 226-231.
• 11. Kocacaliskan, İ., T. Akgul, and S. Erisen, Effect of Juglone on Seed Germination and Seedling Growth of Four Common Vegetables. International Journal of Life Sciences and Biotechnology, 2019. 2(1): p. 43-49.
• 12. Kutty P.C., G.K.A. Parveez and F. Huyop (2011) Agrobacterium tumefaciens –infection strategies for greater transgenic recovery in Nicotiana tabacum cv. TAPM26. International Journal of Agricultural Research 6(2):119-113. ISSN 1816-4897
• 13. Kutty P.C., G.K.A. Parveez and F. Huyop (2010) An easy method for Agrobacterium tumefaciens –mediated gene transfer to Nicotiana tabacum cv. TAPM26. Journal of Biological Sciences 10(6):480-489. ISSN 1727-3048.
• 14. Kumar, N. and M.P. Reddy (2010). Plant regeneration through the direct induction of shoot buds from petiole explants of Jatropha curcas: a biofuel plant. Annals of Applied Biology. 156: 367-375
• 15. Latifian, E., Y.E. Arvas, and Y. Kaya, Tagetes minuta Bitkisinin Mikro Üretimi Üzerine Farklı BAP ve IAA Konsantrasyonlarının Etkileri. International Journal of Life Sciences and Biotechnology, 2018.1(2): p. 96-104.
• 16. Mohammed S. Effects and Quantity Ranges Of Some Auxins On Embryogenic Callus Induction From Upland Rice Cultivars: An Overview. International Journal of Life Sciences and Biotechnology. 2020. DOI: https://doi.org/10.38001/ijlsb.669914
• 17. Ozyigit ıı, M. V. Kahraman, O. Ercan, (2007), Relation between explant age, total phenols and regeneration response in tissue cultured cotton (Gossypium hirsutum L.), Afr J Biotechnol, 6(1), 3-8,
• 18. Ozyigit i ibrahim, (2008), Phenolic changes during in vitro organogenesis of cotton (Gossypium hirsutum L.) shoot tips, Afr J Biotechnol, 7(8), 1145-1150
• 19. Steyn Anita E. , J. A. Brink & L. A. Coetzer (1997) A comparison of culture methods for tobacco (Nicotianatabacum) anthers, South African Journal of Plant and Soil, 14:1, 27-30, DOI: 10.1080/02571862.1997.10635076
• 20. Taha A.M., A. Wagiran, H. Ghazali, F. Huyop and G.K.A. Parveez (2009). In Vitro Regeneration of Garden Balsam, Impatiens balsamina using cotyledons derived from seedlings. Biotechnology. 8(1):44-52. ISSN 1682-296X.