In Vitro Bulb Regeneration from Stem Explants of Endemic Geophyt Muscari aucheri (Boiss.) Baker

Year 2017, Volume: 4 Issue: 3, Special Issue 1, 50 - 54, 25.11.2017

Fethi Ahmet Özdemir , Ömer Kılıç , Eyüp Bağcı

https://doi.org/10.21448/ijsm.356264

Cited By: 1

Abstract

Muscari aucheri (Boiss.) Baker bears beautiful and attractive purplish blue flowers bloom between May and June; it grows stony slopes, mountain pastures an altitude of 1000-3000 meters. The natural populations of Muscari aucheri, is seriously affected by increased environmental pollution, urbanization and grazing. Therefore, this study aimed to accelerate multiplication by devising a strategy for an efficient in vitro bulblet regeneration system of Muscari aucheri using stem explants on MS medium containing 1, 3, 5 mg/l TDZ plus 0, 0.1, 0.2, 0.4 mg/l NAA (12 combinations). The stem explants induced direct bulblet regeneration on explants. Maximum mean number of bulblets per explant was noted on MS medium containing 3 mg/l TDZ + 0.4 mg/l NAA and maximum mean number of bulb diameter was noted on MS medium containing 5 mg/l TDZ + 0.2 mg/l NAA. The regenerated bulblets were isolated from stem explants and cultured on MS medium containing 40 g/l sucrose; where they gained diameter and rooted

Keywords

Muscari aucheri, in vitro, bulb regeneration

In Vitro Bulb Regeneration from Stem Explants of Endemic Geophyt Muscari aucheri (Boiss.) Baker

Abstract

Muscari aucheri (Boiss.) Baker bears beautiful and attractive
purplish blue flowers bloom between May and June; it grows stony slopes,
mountain pastures an altitude of 1000-3000 meters. The natural populations of Muscari aucheri, is seriously affected
by increased environmental pollution, urbanization and grazing. Therefore, this
study aimed to accelerate multiplication by devising a strategy for an
efficient in vitro bulblet
regeneration system of Muscari aucheri
using stem explants on MS medium containing 1, 3, 5 mg/l TDZ plus 0, 0.1, 0.2,
0.4 mg/l NAA (12 combinations). The stem explants induced direct bulblet
regeneration on explants. Maximum mean number of bulblets per explant was noted
on MS medium containing 3 mg/l TDZ + 0.4 mg/l NAA and maximum mean number of
bulb diameter was noted on MS medium containing 5 mg/l TDZ + 0.2 mg/l NAA. The
regenerated bulblets were isolated from stem explants and cultured on MS medium
containing 40 g/l sucrose; where they gained diameter and rooted

Keywords

Muscari aucheri, in vitro, bulb regeneration

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