Effects on Antibacterial and DNA Protection of Organic Dyestuff Extracts Obtained from Hazelnut Nuthusk

Year 2017, Volume: 4 Issue: 3, Special Issue 1, 211 - 217, 25.11.2017

Sibel Bayıl Oğuzkan Bora Karagül , Esra Kütük İbrahim Halil Kılıç , Halil İbrahim Uğraş

https://doi.org/10.21448/ijsm.371555

Abstract

Hazelnut is of great important in agricultural export of our country. It has an annual export of one and a half billion dollars. Every new product that will increase the value of the nut is very important. In recent years, natural dye materials used especially in textile materials are important and those of plant origin are preferred. For this purpose, we have made a natural dye extracts from hazelnut nuthusk after collected, dried and grinded of samples. Some biological activities have been carried out showing the suitability for industrial use of the dye extracts obtained from hazelnut nuthusk. Within the scope of our study, the effect of extracts obtained from the hazelnut nuthusk of the Düzce region was evaluated on anti-bacterial and DNA protection. From the extracts obtained, antibacterial activity tests were analyzed by disk diffusion method using 3 different standard bacterial strains as S. aureus, E. coli and P. aeruginosa. pBR322 plasmid DNA and UV-C method was used for DNA protective activity. Four different extracts of dyestuff has showed antibacterial activities to S. Aureus and two different of them P. aeruginosa but none of the samples antibacterial properties to E. coli bacterial strains. It has been determined that the all of dyestuffs extracts obtained from the hazelnut nuthusk have potential for protection against the effects of UV-C and H2O2 on DNA.

Keywords

UV-C, reflux, H2O2, DNA

Effects on Antibacterial and DNA Protection of Organic Dyestuff Extracts Obtained from Hazelnut Nuthusk

Abstract

Hazelnut is of great important in agricultural export
of our country. It has an annual export of one and a half billion dollars.
Every new product that will increase the value of the nut is very important. In
recent years, natural dye materials used especially in textile materials are
important and those of plant origin are preferred. For this purpose, we have
made a natural dye extracts from hazelnut nuthusk after collected, dried and
grinded of samples. Some biological activities have been carried out showing
the suitability for industrial use of the dye extracts obtained from hazelnut
nuthusk. Within the scope of our study, the effect of extracts obtained from
the hazelnut nuthusk of the Düzce region was evaluated on anti-bacterial and
DNA protection. From the extracts obtained, antibacterial activity tests were
analyzed by disk diffusion method using 3 different standard bacterial strains
as S. aureus,
E. coli and P. aeruginosa.  pBR322 plasmid DNA and UV-C method was used for DNA protective activity.
Four different extracts of dyestuff has showed
antibacterial activities to S. Aureus and two different of them P. aeruginosa but none of the
samples antibacterial properties to E. coli bacterial strains. It has
been determined that the all of dyestuffs extracts obtained from the hazelnut
nuthusk have potential for protection against the effects of UV-C and H₂O₂
on DNA.

Keywords

UV-C, reflux, H2O2, DNA

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