Microwave-assisted synthesis of N-benzylidene-4-fluoroaniline and N-benzylidene-4-Nitroaniline and their inhibitory activities on hCA isoenzymes

Year 2019, Volume: 6 Issue: 1, 38 - 48, 16.03.2019

Hülya Çelik , Aynur Babagil

https://doi.org/10.21448/ijsm.479108

Cited By: 1

Abstract

In this study,
N-benzylidene-4-fluoroaniline (3a), N-benzylidene-4-nitroaniline (3b) as a
result of condensation of benzaldehyde (1) and 4-fluoroaniline (2a),
4-nitroaniline (2b) using a microwave method was synthesized. The structures of
the synthesized compounds were characterized by Fouirer Transform İnfrared
Spektrofotometre (FTIR spectroscopy), Nuclear Magnetic Resonance (NMR
spectroscopy) and elemental analysis methods. The prepared compounds were
tested for their inhibitory effects on carbonic anhydrase isoenzymes (hCA-I and
hCA-II). The results showed that the synthesized compounds 3a and 3b had a
strong inhibitory effect on hCA-I and hCA-II enzymes activity in vitro. By
testing these compounds in other CA isoenzymes, more effective CA inhibitors
can be formed. Thus, new therapeutic applications can be made for enzyme
activators in the near future.

Keywords

Schiff, microwave, fluoroaniline, nitroaniline

Microwave-assisted synthesis of N-benzylidene-4-fluoroaniline and N-benzylidene-4-Nitroaniline and their inhibitory activities on hCA isoenzymes

Abstract

In this study, N-benzylidene-4-fluoroaniline (3a), N-benzylidene-4-nitroaniline (3b) as a result of condensation of benzaldehyde (1) and 4-fluoroaniline (2a), 4-nitroaniline (2b) using a microwave method was synthesized. The structures of the synthesized compounds were characterized by Fouirer Transform İnfrared Spektrofotometre (FTIR spectroscopy), Nuclear Magnetic Resonance (NMR spectroscopy) and elemental analysis methods. The prepared compounds were tested for their inhibitory effects on carbonic anhydrase isoenzymes (hCA-I and hCA-II). The results showed that the synthesized compounds 3a and 3b had a strong inhibitory effect on hCA-I and hCA-II enzymes activity in vitro. By testing these compounds in other CA isoenzymes, more effective CA inhibitors can be formed. Thus, new therapeutic applications can be made for enzyme activators in the near future.

Keywords

Schiff, Microwave, Fluoroaniline, Nitroaniline, Inhibitor

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