Antiproliferative and antioxidant potential of methanol extracts of aerial parts of Colchicum boissieri and Colchicum balansae

Year 2022, Volume: 9 Issue: 2, 149 - 157, 15.06.2022

Mehlika Alper

https://doi.org/10.21448/ijsm.1056920

Cited By: 2

Abstract

The antiproliferative and antioxidant activities, and total phenolic, flavonoid, and tannin contents of methanol extracts obtained from the aerial parts of Colchicum boissieri and Colchicum balansae were investigated in the present study. The evaluation of the antiproliferative effects of the extracts under study was carried out using the MTT assay. The antioxidant potentials of the extracts were assigned by using several assays (FRAP, CUPRAC, DPPH, ABTS, and β-carotene). The antiproliferative effects of both extracts on Caco-2 cells appeared to be generally similar for the incubation times tested. The antioxidant potential was found to be higher in the extract of C. boissieri (FRAP: on average 1.39 mg TE/g, CUPRAC: on average 10.06 mg TE/g, DPPH: on average IC50= 0.59 mg/mL, ABTS: on average IC50= 0.267 mg/mL, β-carotene: on average 78.58%) than the extract of C. balansae. In terms of total phenolic, flavonoid and tannins contents, C. boissieri extract (on average 1.97 mg GAE/g, 8.65 mg QE/g and 4.75 mg CE/g, respectively) was determined to be richer than C. balansae extract. The results suggest that both extracts have some biological properties for pharmaceutical applications. Further studies may contribute to the use of these plants for various purposes such as natural antioxidant sources or cancer agents.

Keywords

Colchicum boissieri, Colchicum balansae, Methanolic extract, Antiproliferative, Antioxidant

References

• Al-Snafi, A.E. (2016). Medicinal importance of Colchicum candidum-A review. The Pharmaceutical and Chemical Journal, 3(2), 111-117.
• Alkadi, H. (2020). A review on free radicals and antioxidants. Infectious Disorders-Drug Targets (Formerly Current Drug Targets-Infectious Disorders), 20(1), 16-26. https://doi.org/10.2174/1871526518666180628124323
• Amarowicz, R., Pegg, R.B., Rahimi-Moghaddam, P., Barl, B., & Weil, J.A. (2004). Free-radical scavenging capacity and antioxidant activity of selected plant species from the Canadian prairies. Food Chemistry, 84(4), 551 562. https://doi.org/10.1016/S0308 8146(03)00278-4
• Apak, R., Güçlü, K., Özyürek, M., Esin Karademir, S., & Erçağ, E. (2006). The cupric ion reducing antioxidant capacity and polyphenolic content of some herbal teas. International Journal of Food Sciences and Nutrition, 57(5 6), 292 304. https://doi.org/10.1080/09637480600798132
• Artun, F.T., Karagoz, A., Ozcan, G., Melikoglu, G., Anil, S., Kultur, S., & Sutlupinar, N. (2016). In vitro anticancer and cytotoxic activities of some plant extracts on HeLa and Vero cell lines. Journal of B.U.ON.: official journal of the Balkan Union of Oncology, 21(3), 720-725.
• Aryal, S., Baniya, M.K., Danekhu, K., Kunwar, P., Gurung, R., & Koirala, N. (2019). Total phenolic content, flavonoid content and antioxidant potential of wild vegetables from Western Nepal. Plants, 8(4), 96. https://doi.org/10.3390/plants8040096
• Azadbakht, M., Davoodi, A., Hosseinimehr, S.J., Keighobadi, M., Fakhar, M., Valadan, R., Faridnia, R., Emami, S., Azadbakht, M., & Bakhtiyari, A. (2020). Tropolone alkaloids from Colchicum kurdicum (Bornm.) Stef. (Colchicaceae) as the potent novel antileishmanial compounds; purification, structure elucidation, antileishmanial activities and molecular docking studies. Experimental Parasitology, 213, 107902. https://doi.org/10.1016/j.exppara.2020.107902
• Becer, E., Hanoğlu, D.Y., Kabadayı, H., Hanoğlu, A., Vatansever, S., Yavuz, D.Ö., Meriçli, F., & Meriçli, A.H. (2019). The effect of Colchicum pusillum in human colon cancer cells via Wnt/β catenin pathway. Gene, 686, 213 219. https://doi.org/10.1016/j.gene.2018.11.047
• Bekir, J., Mars, M., Souchard, J.P., & Bouajila, J. (2013). Assessment of antioxidant, anti-inflammatory, anti-cholinesterase and cytotoxic activities of pomegranate (Punica granatum) leaves. Food and Chemical Toxicology, 55, 470 475. https://doi.org/10.1016/j.fct.2013.01.036
• Benzie, I.F., & Strain, J.J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. Analytical Biochemistry, 239(1), 70-76. https://doi.org/10.1006/abio.1996.0292
• Birben, E., Sahiner, U.M., Sackesen, C., Erzurum, S., & Kalayci, O. (2012). Oxidative stress and antioxidant defense. The World Allergy Organization Journal, 5(1), 9-19. https://doi.org/10.1097/WOX.0b013e3182439613
• Dagdeviren Ozsoylemez, O., & Ozcan, G. (2021). Effects of Colchicum baytopiorum leaf extract on cytotoxicity and cell death pathways in C-4 I and Vero cell lines. Journal of B.U.ON.: Official Journal of the Balkan Union of Oncology, 26(3), 1135-1137.
• Davoodi, A., Azadbakht, M., Hosseinimehr, S.J., Emami, S., & Azadbakht, M. (2021). Phytochemical Profiles, Physicochemical Analysis, and Biological Activity of Three Colchicum Species. Jundishapur Journal of Natural Pharmaceutical Products, 16(2), e98868.
• Gulsoy-Toplan, G., Goger, F., Yildiz-Pekoz, A., Gibbons, S., Sariyar, G., & Mat, A. (2018). Chemical Constituents of the Different Parts of Colchicum micranthum and C. chalcedonicum and their Cytotoxic Activities. Natural Product Communications, 13(5), 535-538. https://doi.org/10.1177/1934578X1801300506
• Hailu, T., Sharma, R., Mann, S., Gupta, P., Gupta, R.K., & Rani, A. (2021). Determination of Bioactive Phytochemicals, Antioxidant and Anti-inflammatory Activity of Colchicum autumnale L. (Suranjanshireen). Indian Journal of Natural Products and Resources, 12(1), 52-60.
• Kamiloglu, S., Sari, G., Ozdal, T., & Capanoglu, E. (2020). Guidelines for cell viability assays. Food Frontiers, 1(3), 332-349. https://doi.org/10.1002/fft2.44
• Kiliç, I., Yesiloglu, Y., & Bayrak, Y. (2014). Investigation on the Antioxidant Activity of Roots and Stem of Colchicum turcicum L. Asian Journal of Chemistry, 26(1), 5-9.
• Kooti, W., Servatyari, K., Behzadifar, M., Asadi-Samani, M., Sadeghi, F., Nouri, B., & Zare Marzouni, H. (2017). Effective Medicinal Plant in Cancer Treatment, Part 2: Review Study. Journal of Evidence-Based Complementary & Alternative Medicine, 22(4), 982-995. https://doi.org/10.1177/2156587217696927
• Kratz, F., Müller, I. A., Ryppa, C., & Warnecke, A. (2008). Prodrug strategies in anticancer chemotherapy. ChemMedChem: Chemistry Enabling Drug Discovery, 3(1), 20-53. https://doi.org/10.1002/cmdc.200700159
• Lobo, V., Patil, A., Phatak, A., & Chandra, N. (2010). Free radicals, antioxidants and functional foods: Impact on human health. Pharmacognosy Reviews, 4(8), 118. https://doi.org/10.4103/0973-7847.70902
• Lourenço, S.C., Moldão-Martins, M., & Alves, V.D. (2019). Antioxidants of Natural Plant Origins: From Sources to Food Industry Applications. Molecules (Basel, Switzerland), 24(22), 4132. https://doi.org/10.3390/molecules24224132
• Mammadov, R., Düşen, O., Demir, D.U., & Köse, E. (2009). Antioxidant and antimicrobial activities of extracts from tubers and leaves of Colchicum balansae Planchon. Journal of Medicinal Plants Research, 3(10), 767-770.
• Mosmann, T. (1983). Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxic assay. Journal of Immunological Methods, 65, 55-63. https://doi.org/10.1016/0022-1759(83)90303-4
• Munteanu, I.G., & Apetrei, C. (2021). Analytical Methods Used in Determining Antioxidant Activity: A Review. International Journal of Molecular Sciences, 22(7), 3380. https://doi.org/10.3390/ijms22073380
• Ozsoylemez, O. D., Ozturk, M., Sutlupinar, N., Kayacan, S., Tuncdemir, M., & Ozan, G. (2016). The effects of Colchicum baytopiorum on regulatory genes of apoptotic and autophagic cell death in HeLa cells. Current Pharmaceutical Biotechnology, 17(15), 1369-1376. https://doi.org/10.2174/1389201018666161109113543
• Parasuraman, S. (2018). Herbal drug discovery: challenges and perspectives. Current Pharmacogenomics and Personalized Medicine (Formerly Current Pharmacogenomics), 16(1), 63 68. https://doi.org/10.2174/1875692116666180419153313
• Paul, D. (2020). The systemic hallmarks of cancer. Journal of Cancer Metastasis and Treatment, 6, 29. https://doi.org/10.20517/2394-4722.2020.63
• Pırıldar, S., Sütlüpınar, N., Atasever, B., Erdem-Kuruca, S., Papouskova, B., & Šimánek, V. (2010). Chemical constituents of the different parts of Colchicum baytopiorum and their cytotoxic activities on K562 and HL60 cell lines. Pharmaceutical Biology, 48(1), 32-39. https://doi.org/10.3109/13880200903029373
• Pisoschi, A.M., & Negulescu, G.P. (2011). Methods for Total Antioxidant Activity Determination: A Review. Biochemistry & Analytical Biochemistry, 1, 106. https://doi.org/10.4172/2161-1009.1000106
• Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., & Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26(9 10), 1231 1237. https://doi.org/10.1016/S0891-5849(98)00315-3
• Rocchetti, G., Senizza, B., Zengin, G., Okur, M.A., Montesano, D., Yildiztugay, E., Lobine D., Mahomoodally, M.F., & Lucini, L. (2019). Chemical Profiling and Biological Properties of Extracts from Different Parts of Colchicum szovitsii subsp. szovitsii. Antioxidants, 8(12), 632. https://doi.org/10.3390/antiox8120632
• Senizza, B., Rocchetti, G., Okur, M.A., Zengin, G., Yıldıztugay, E., Ak, G., MontesanoD., Lucini, L. (2020). Phytochemical Profile and Biological Properties of Colchicum triphyllum (Meadow Saffron). Foods, 9(4), 457. https://doi.org/10.3390/foods9040457
• Sevim, D., Senol, F.S., Budakoglu, E., Orhan, I.E., Sener, B., & Kaya, E. (2010). Studies on anticholinesterase and antioxidant effects of samples from Colchicum L. genus of Turkish origin. FABAD J. Pharm. Sci., 35, 195-201.
• Souri, E., Amin, G.R., Jalalizadeh, H., & Barezi, S. (2008). Screening of Thirteen Medicinal Plant Extracts for Antioxidant Activity. Iranian Journal of Pharmaceutical Research, 7(2), 149-154.
• Suica-Bunghez, I.R., Ion, R.M., Teodorescu, S., Sorescu, A.A., Stirbescu, R.M., & Stirbescu, N.M. (2017). Fitochemical and Antioxidant Characterization of Autumn Crocus (Colchicum autumnale) Flowers and Roots Plant Extracts. Journal of Science and Arts, 17(3), 539-546.
• Sung, H., Ferlay, J., Siegel, R.L., Laversanne, M., Soerjomataram, I., Jemal, A., & Bray, F. (2021). Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA: a Cancer Journal for Clinicians, 71(3), 209-249. https://doi.org/10.3322/caac.21660
• Toplan, G.G., Gürer, Ç., & Mat, A. (2016). Importance of Colchicum species in modern therapy and its significance in Turkey. Journal of Faculty of Pharmacy of Istanbul University, 46(2), 129-144.
• Turan, M., & Mammadov, R. (2018). Antioxidant, antimicrobial, cytotoxic, larvicidal and anthelmintic activities and phenolic contents of Cyclamen alpinum. Pharmacology & Pharmacy, 9(04), 100. https://doi.org/10.4236/pp.2018.94008
• Xiao, F., Xu, T., Lu, B., & Liu, R. (2020). Guidelines for antioxidant assays for food components. Food Frontiers, 1(1), 60-69. https://doi.org/10.1002/fft2.10
• Xu, D.P., Li, Y., Meng, X., Zhou, T., Zhou, Y., Zheng, J., Zhang, J.J., & Li, H.B. (2017). Natural Antioxidants in Foods and Medicinal Plants: Extraction, Assessment and Resources. International Journal of Molecular Sciences, 18(1), 96. https://doi.org/10.3390/ijms18010096
• Zehiroglu, C., & Sarikaya, S.B.O. (2019). The importance of antioxidants and place in today’s scientific and technological studies. Journal of Food Science and Technology, 56(11), 4757-4774. https://doi.org/10.1007/s13197-019-03952-x