Chemical content profile and antioxidant activity of Rhododendron ponticum L. (Ericaceae) extracts

Year 2024, Volume: 11 Issue: 3, 507 - 513, 29.08.2024

Yusuf Sıcak , İrfan Öztürk Bihter Şahin , Dilaycan Çam , Cansel Cakır , Mehmet Öztürk

https://doi.org/10.21448/ijsm.1382522

Abstract

Rhododendron species (Ericaceae) is considered the most diverse group, with more than 1200 species famous for their colorful flowers. Rhododendron, also known as azalea, is a flowering tree in the Ericaceae family. Herein, in vitro antioxidant activities of acetone and methanol extracts of Rhododendron ponticum leaves were studied along with their phenolic contents using High-Performance Liquid Chromatography with a Diode-Array Detector (HPLC-DAD). Antioxidant activity was performed spectrophotometrically using ABTS·+, DPPH·, CUPRAC, and β-carotene/linoleic acid assays. Acetone extract showed better antioxidant activity than methanol extract in all tests. The HPLC-DAD analysis revealed fifteen phenolic compounds, of which seven were common for both extracts. Catechin (25.80 and 33.08 mg/g extract, respectively) and epicatechin (31.15 and 26.54 mg/g extract, respectively) were calculated as major phenolic components in acetone and methanol extracts.

Keywords

Rhododendron ponticum, HPLC-DAD, Phenolic compound, Antioxidant activity

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