Preparation and characterization of chitosan nanoparticles with extracts of Rheum ribes, evaluation of biological activities of extracts and extract loaded nanoparticles

Year 2024, Volume: 11 Issue: 4, 751 - 764, 03.11.2024

Murat Doğan Ümit Muhammet Koçyiğit , Duygu Taşkın , Beyza Nur Yılmaz , Turgut Taşkın

https://doi.org/10.21448/ijsm.1425978

Abstract

The biological activities of different parts of the Rheum ribes plant were evaluated comparatively. Extracts showing strong biological activity were identified and it was determined which of the extract-loaded nanoparticles showed stronger activity. Cytotoxic activity of R. ribes extracts was calculated on glial (C6) and fibroblast (NIH 3T3) cells using XTT assay. Spectrophotometry was used to evaluate the impact of these compounds on the enzyme activities of human carbonic anhydrase I and II (hCA I and hCA II). The findings showed that chitosan NPs with extracts loaded on them have a lower IC50 value and more cytotoxic activity in C6 cells than chitosan NPs with only extracts. R. ribes young shoots ultrasonic methanol extract (RYU) was shown to have the strongest antiproliferative efficacy against C6 cells. Results showed that RYU and ultrasonic methanol extract of R. ribes radix (RRU) were determined as the best carbonic anhydrase inhibitors. According to results of particle size, encapsulation efficiency, and release studies of chitosan NPs, it has been observed that they are suitable for application. At a concentration of 10 µg/mL, it was found that none of the R. ribes extracts exhibited cytotoxic action toward the NIH 3T3 cell line. According to results of particle size, encapsulation efficiency, and release studies of chitosan NPs, it has been observed that they are suitable for application. It was observed that none of the extracts of R. ribes at a concentration of 10 µg/mL showed cytotoxic activity in the NIH 3T3 cell line.

Keywords

Chitosan nanoparticles, Rheum ribes, Bioactivity, Cytotoxicity, Characterization of nanoparticles

Supporting Institution

This research was carried out at the Cumhuriyet University Faculty of Medicine Research Center, Marmara University Faculty of Pharmacy Laboratory of Pharmacognosy, and Cumhuriyet University Faculty of Pharmacy.

Thanks

Thanks to Assist. Prof. Dr. Gizem BULUT who works at Marmara University, Department of Pharmaceutical Botany, for his contribution to the identification of Rheum ribes plant.

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