Cytotoxicity of Some Retail Food Supplements

Year 2020, Volume: 1 Issue: 3, 131 - 136, 15.12.2020

Pınar Ağyar Yoldaş , Taner Yoldaş , Nisa Sipahi

Abstract

Objective: Medicinal plants and products obtained from medicinal plants are widely used in the treatment of various diseases in our country as well as all over the world. However, scientific data on the biological effects and mechanisms of action of most extracts from medicinal plants are still lacking. Because of that interest in scientific research of the biological effects of medicinal plants and products obtained from medicinal plants is increasing day by day. In addition, the reliability of these products in terms of use apart from their effectiveness is very important for public health. In this study, we aimed to investigate the cytotoxicity of commercially marketed AYM-net® natural herbal food supplements in L929 (mouse fibroblast) cells.
Material-Method:
In this study, the WST-1 cell proliferation test protocol was applied to examine the cytotoxicity of 19 different products on the market belonging to AYM-net® in L929 cell lines and the results were evaluated according to Elisa microplate reading data. The products were also tested at concentrations of 0.02, 0.03, 0.04, 0.05, 0.06 µg / ml, taking into account the amounts of daily use.
Results: When the results were evaluated, 19 different AYM-net® food supplement products sold on the market were cytotoxic in the L929 cell line, in the concentration range of 0.02-0.06 µg/mL, it was observed that the products were not toxic at these doses
Conclusion: As a result, different doses of AYM-net® herbal products did not show toxicity on L929 cells. Doses used are within the reliable range.
Keywords: L929, cell culture, cytotoxicity, food supplement, herbal mixture

Keywords

L929, CELL CULTURE, CYTOTOXİCİTY, FOOD SUPPLEMENT, HERBAL MİXTURE

References

• Nguta J.M, Appiah-Opong, R, Nyarko, AK, et al. Antimycobacterial and cytotoxic activity of selected medicinal plant extracts. J. Ethnopharmacol. 2016;182:10-15.
• Ogbole O, Segun P.A, & Adeniji, A. J. In vitro cytotoxic activity of medicinal plants from Nigeria ethnomedicine on Rhabdomyosarcoma cancer cell line and HPLC analysis of active extracts. BMC Complement Altern. Med. 2017;17(1), 494.
• Csepregi R, Temesfői V, Das S, et al. Cytotoxic, antimicrobial, antioxidant properties and effects on cell migration of phenolic compounds of selected transylvanian medicinal plants. Antioxidants. 2020; 9(2), 166.
• Demir T, Akpınar Ö. (). Biological Activities of Phytochemicals in Plants. Turkish Journal of Agriculture-Food Science and Technology. 2020;8(8):1734-1746.
• Verschaeve L, Kestens V, Taylor J.L.S, et al.. Investigation of antiMutagenic effects of selected South African medicinal plant extracts. Toxicol. In Vitro. 2004;18:29-35.
• Brian W.R. Isolation and structure elucidation of cytotoxic natural products from Suriname and Madagascar. Master thesis, Virginia Polytechnic Institute and State University. 2002.
• Doyle A, Griffiths JB. Cell and Tissue Culture: Laboratory Procedures in Biotechnology. Scientific Consultancy & Publishing. 1998;62-64.
• Alim E.Ç, Yarsan E. Böbreklere yönelik zehirli maddeler. Vet Farm Toks Dern Bult. 2019;10(2), 77-90.
• Mensah M.L, Komlaga G, Forkuo A.D, et al. Toxicity and safety implications of herbal medicines used in Africa. Herb. Med. 2019;63, 1992-0849.
• Debelle F.D, Vanherweghem J.L, Nortier J.L. Aristolochic acid nephropathy: a worldwide problem. Kidney Int. 2008;74(2):158-169.
• Zhou X, Li C.G, Chang D, Bensoussan A. Current status and major challenges to the safety and efficacy presented by Chinese herbal medicine. Medicines. 2019;6(1):14.
• Liu C, Fan H, Li Y, Xiao X. Research advances on hepatotoxicity of herbal medicines in China. BioMed Res Int. 2016;7150391.
• Sipahi N, Karakaya E, Ikiz S. Phenotypic and genotypic investigation of the heavy metal resistance in Escherichia coli isolates recovered from cattle stool samples. Turk J Vet Anim Sci. 2019;43(5):684-691.
• Albuz Ö. Investigation of cytotoxic effects of Curcuma longa, Zingiberaceae and Dianthus caryophyllus, which are commonly used as food supplements in daily life. Kocatepe Vet. J. 2019;12(3), 351-356.
• Yaşar M, ŞenoğuL O, Şirin N, et al. Effect of My Guard® Food Supplement on Acute and Subacute Toxicity on Rats. Int J Tradit Complement Med Res. 2020;1(1):25-32.
• Smina T.P, Mathew J, Janardhanan K.K, et al. Antioxidant activity and toxicity profile of total triterpenes isolated from Ganoderma lucidum (Fr.) P. Karst occurring in South India. Environ. Toxicol. Pharmacol. 2011;32(3):438-446.
• Artıran MÖ, Dönmez D.B, Bayçu C, Yılmaz H. Gentamisin’le Testis Hasarı Oluşturulan sıçanlarda C Vitamininin Koruyucu Etkisi. Osmangazi Tıp Derg. 2017;39(2):32-39.
• Brown A.C. Liver toxicity related to herbs and dietary supplements: Online table of case reports. Part 2 of 5 series. Food Chem. Toxicol. 2017;107: 472-501.
• Tokur O, Aksoy A. In vitro sitotoksisite testleri. Harran Üniv Vet Fak Derg. 2017;6(1):112-118.