The Use of Natural Preservative Propolis and Hypericum perforatum Oil in Herbal Cream Production

Year 2021, Volume: 2 Issue: 1, 27 - 35, 15.04.2021

Gülşah Aydın , Neslihan Şirin , Meral Kekeçoğlu , Tuğba Türken , Nisa Sipahi , Haydar Göksu

Abstract

Objective:
Consumers' preference for products produced with natural additives has accelerated the search for natural substances that are alternative to synthetic materials in cosmetics and food production. The aim of the study is to obtain a herbal cream by using the natural preservative effect of propolis and the moisturizing effect of St. John’s wort (Hypericum perforatum L.) fixed oil of Hypericum perforatum plant which grows naturally in our country.
Material-Method:
The chemical content of the obtained propolis ethanolic extract was determined by LC-MS/MS analysis. Hypericum perforatum fixed oil was obtained by maceration and the chemical content of the oil was determined by GC-MS analysis. Herbal cream was prepared using PEG400 to facilitate dispersion of Hypericum perforatum oil and propolis extract obtained in water-based cream formulations and to provide a creamy consistency. Microbiological stability tests were carried out with reference to ISO21149, ISO16212, ISO18416, ISO22717 and ISO21150 standards and ISO11930: 2012 standard for challenge test (preservative efficacy tests).
Results:
The total phenolic content of propolis ethanol extract was determined as 66.096 ± 1.546 mg gallic acid equivalent (GAE) / mL sample, dry weight and flavonoid content as 13.375 ± 0.185 quercetin equivalent (QE) / mL sample. An extract rich in phenolic components such as quercetin, p-coumaric acid, feluric acid, trans-cinnamic acid, benzoic acid was obtained. Component analysis showed that propolis ethanolic extract could show preservative efficiency. The presence in the GC-MS analysis of Hypericum perforatum oil that it contains fatty acids widely used in the cosmetic and pharmaceutical industry clearly demonstrated that the oil can be used safely in cream formulations.
Conclusion:
The test results of the creams prepared using Hypericum perforatum and propolis clearly revealed that the obtained propolis extract provided protective efficacy. The thickener property of PEG400, which is used to ensure homogeneous distribution, reduced the need for an extra thickener additive.

Keywords

Natural Preservative, Herbal Cream, Propolis, Hypericum perforatum L., St. John's wort

Supporting Institution

Duzce University

Project Number

2019.01.01.1033

Thanks

The study was carried out within the scope of the project numbered 2019.01.01.1033, supported by Duzce University Scientific Research Projects Unit. The authors would like to thank Duzce University Scientific Research Projects Unit for financial support.

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