Investigation of Antitumor Activity of Fenugreek Paste Extracts

Year 2024, Volume: 5 Issue: 2, 101 - 111, 03.09.2024

Serife Alpa , Özlem Özçelik , Seher Yilmaz , Tolga Ertekin , Mehtap Nisari , Gökçe Şeker Karatoprak , Harun Ülger

https://doi.org/10.53811/ijtcmr.1433242

Abstract

Objective: Fenugreek paste, which contains fenugreek and some spices, is a widely consumed food in Kayseri and the surrounding region of Turkey. In this study, the antitumor effect of an extract obtained from fenugreek paste was investigated in Balb/C mice bearing Ehrlich ascites tumor (EAT).
Materials and Methods: The concentration of fenugreek paste extract in vivo was 200 and 400 mg/kg and in vitro was 250, 500 and 1000 µg/ml. At the end of the experiment, the volume of abdominal ascites fluid was measured and cells were counted.
Results: Fenugreek paste extract delayed weight gain due to EAT cell proliferation in the treatment groups and caused a significant decrease in the number of cells in the ascites fluid in the 400 mg/kg fenugreek paste extract group (47.28 × 106) compared to the control group (67.60 × 106; p = 0.041). Histopathological analysis showed that EAT cells intensely adhered to tissues from the control group and adhered less in the treatment groups. The decrease was more significant in the 400-mg/kg fenugreek extract group. After 24-hour culture, there was a significant difference in EAT cells between the control group (5.9 ± 0.2) and the 250-, 500- and 1000-µg/ml fenugreek paste extract treatment groups (5.7 ± 0.2, 5.7 ± 0.2, and 5.6 ± 0.1, respectively; p = 0.013).
Conclusion: The study results suggested that fenugreek paste extract had an antitumor effect on EAT cells.

Keywords

Ehrlich ascites tumor, Fenugreek paste, Fenugreek, Red pepper, Garlic

Ethical Statement

Erciyes Üniversitesi Hayvan Deneyleri Yerel Kurulu tarafından Etik onay alınmıştır.

Supporting Institution

Erciyes Üniversitesi

Project Number

The project that the code TDK-2014-5325 was supported by Erciyes University Scientific Research Projects Unit.

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