Ratlarda Pars Timpani Perforasyonunda Nar Ekstratının Mast Hücre Sayısına Etkisi

Year 2018, Volume: 1 Issue: 1, 5 - 8, 22.12.2018

Tuğrul Ertuğrul , Şerife Tütüncü

Abstract

Kulak zarının temel fonksiyonu ses titreşimlerini dış ortamdan alarak kulak kemikçiklerine iletmektir. Yara iyileşmesinde mast hücrelerinin sayılarının arttığı bildirilmektedir. Bu çalışmada, deneysel pars tensa perforasyonunda mast hücrelerinin sayı ve heterojenitesi incelenmiştir. Pars tensa %10 formol’de tespit edilip yıkandıktan sonra rutin histolojik doku takibi prosedürlerinden geçirilerek parafinde bloklandı. Alınan kesitler toluidin blue ve alcian blue/safranin O kombine boya metodu ile boyandı. Tüm gruplarda, pars tensada mast hücreleri metakromatik, alcian blue (+) ve safranin O (+) boyandı. İyileşme sürecinde en fazla sayıda mast hücresine nar ekstratı verilmiş grupta rastlandı. 

Keywords

Pars tensa, perforasyon, nar ekstratı, mast hücresi

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