Application of Experimental Design Method in Bioprocess Studies

Year 2020, Volume: 1 Issue: 2, 188 - 197, 31.12.2020

Meryem Sena Akkuş , Sema Karabudak

Abstract

Experimental design methods are the techniques that reduce the costs of the experiments performed, increase the reliability of the results, and reach the desired goal in a short time with less experimentation. In the work of biological processes, there are many factors aimed at the desired goal, that affect the target directly or indirectly. It is possible to reach the most accurate and accurate result with the appropriate experimental design method. In this study, information is given about how the full factorial design, Taguchi method, Box-Behnken design, central composite design, and Plackett-Burman design methods, which are among the widely used experimental design methods in the literature, reach the most appropriate value conditions in implementations and application examples of these methods in bioprocess studies.

Keywords

Experimental design methods, Process optimization, Bioprocess

Biyoproses Çalışmalarında Deneysel Tasarım Yönteminin Uygulanması

Abstract

Deney tasarımı yöntemleri yapılan deneylerin maliyetlerini düşüren, sonuçların güvenilirliğini artıran, az deneyle istenen hedefe kısa sürede ulaştıran tekniklerdir. Biyolojik proseslerin çalışmalarında istenen hedefe yönelik, hedefi doğrudan veya dolaylı olarak etkileyen birçok faktör bulunmaktadır. Uygun deney tasarım yöntemiyle gerçeğe en yakın ve hassas sonuca ulaşmak mümkündür. Yapılan bu çalışmada literatürde yaygın olarak kullanılan deney tasarım yöntemlerinden tam faktöriyel dizaynı, Taguchi metodu, Box–Behnken tasarımı, merkezi kompozit tasarımı ve Plackett–Burman tasarım yöntemlerinin uygulamalarda en uygun değer koşullarına nasıl ulaştığını ve biyoproses çalışmalarında bu yöntemlerin uygulama örnekleri hakkında bilgi verilmiştir.

Keywords

Deneysel tasarım yöntemleri, Proses optimizasyonu, biyoproses

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