Beyin Hasarı ile Restoratif Materyaller Arasındaki Bağlantının İncelenmesi: Bir İn-vivo Çalışma

Year 2020, Volume: 8 Issue: 3, 664 - 675, 30.11.2020

Selina Aksak Karameşe , İsmail Can , Mustafa Duzyol , Nilgün Seven , Mevlüt Albayrak , Fadime Atalay , Esra Düzyol , Cemal Gündoğdu

https://doi.org/10.33715/inonusaglik.718551

Abstract

Bu çalışmanın amacı, rezin kompozit, amalgam ve cam iyonomer gibi restoratif materyallerin beyin dokusu üzerindeki olası etkilerini araştırmaktır. Otuziki adet Wistar albino sıçan alındı ve restoratif malzemeler boyun bölgesinin arkasına yerleştirildi. 8 hafta sonra beyin dokuları çıkarıldı ve nükleer faktör kappa B (NF-Κb)/p65 ve hematoksilin & eosin boyama metodları ile analiz yapıldı. Ayrıca, beyin dokusunda; süperoksit dismutaz, katalaz, lipid peroksidaz ve glutatyon düzeyleri tespit edildi. Deney gruplarında kontrol gruplarına göre immünpozitif hücre boyanması daha yoğun olarak gözlendi, ayrıca bu gruplarda nöronal dejenerasyon saptandı. Test sonuçları, tüm restoratif materyal gruplarında kontrol grubuna kıyasla artmış oksidatif stres gösterdi. Kullanılan restoratif materyallerin üçü de beyin dokusu üzerinde ciddi sitotoksik etkilere neden oldu. Ek olarak, beyin dokusunda oksidatif stres oluşmuş olabilir. Restoratif maddelerinin içerdikleri ağır metal bileşikler, restoratif maddelerin sitotoksik etkilerini gösterecek şekilde nöronal dejenerasyona neden oldu ve ayrıca oksidatif strese yol açmış olabilirler.

Keywords

: Beyin Dejenerasyonu, Cam İyonomer Siman, Dental Amalgam, Oksidatif Stres, Rezin Kompozit

Supporting Institution

TÜBİTAK

Project Number

114S881

THE EXAMINATION OF THE RELATIONSHIP BETWEEN BRAIN DAMAGE AND DENTAL RESTORATIVE FILLING MATERIALS: AN IN-VIVO STUDY

Abstract

The aim of this study is to investigate the possible effects of restorative materials such as resin composite, amalgam and glass ionomer on brain tissue. Thirty-two Wistar albino rats were taken and restorative materials were put in the neck region at the back. After 8 weeks, the brain tissues were removed and analyzed using nuclear factor kappa B (NF-κB)/p65 and hematoxylin & eosin staining methods. Further, the levels of superoxide dismutase, catalase, lipid peroxidase, and glutathione were determined in the brain tissues. More intense staining of immunopositive cells was observed in the restorative material groups than the control group, also neuronal degeneration was detected in these groups. Results of the tests indicated increased oxidative stress in all the restorative material groups compared with the control group. All three dental restorative materials exhibited cytotoxic effects on the brain tissue. Additionally, oxidative stress may have occurred in the brain tissue. The heavy metal compounds in the restorative materials caused neuronal degeneration and may also have caused oxidative stress in the brain tissue, indicating the cytotoxic effects of dental restorative materials.

Keywords

brain degeneration, dental amalgam, glass ionomer, resin composite, oxidative stress

Project Number

114S881

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