Sıçanlarda Doksorubisin Kaynaklı Böbrek Hasarı Üzerine Selenyum ve N-(p-Amilsinnamoil) Antranilik Asit’in Etkisi

Year 2023, Volume: 11 Issue: 1, 1181 - 1191, 17.03.2023

Kenan Yıldızhan , Zübeyir Huyut , Fikret Altındağ , Bünyamin Uçar

https://doi.org/10.33715/inonusaglik.1174849

Cited By: 1

Abstract

Doksorubisin (DOXR) en önemli antitümör ilaçlardan biridir. Bununla birlikte, birçok organda ciddi istenmeyen yan etkileri, DOXR'in klinikteki kullanımını sınırlamaktadır. Bu çalışma, DOXR uygulanan sıçanlarda Selenyum (Se) ve N-(p-Amilsinnamoil) antranilik asit'in (ACA) böbrek dokusu üzerindeki etkisini araştırmayı amaçlamıştır. Çalışmada kullanılacak sıçanlar altı gruba ayrıldı (n=10); Kontrol, DMSO, DOXR, DOXR+Se, DOXR+ACA ve DOXR+Se+ACA. Çalışmanın sonunda, sıçanların intrakardiyak kanı ve ayrıca böbrek dokuları alındı. Sıçanların serum örneklerinde üre ve kreatin seviyelerine bakıldı. Ayrıca böbrek dokusunda histopatolojik inceleme H&E boyama ile ve 8-OHdG ekspresyonu immünohistokimyasal analiz ile belirlendi. Se ve ACA tedavilerinden sonra, serum örneklerinde DOXR ile artan üre ve kreatin seviyeleri azaldı (p< 0.05). DOXR uygulanan grupta, glomerüler atrofi, tübüler ve glomerüler dilatasyon, damarlarda tıkanıklık ve tübüler epitel hücrelerinde dejenerasyon gözlenirken, Se ve ACA ile tedavi gruplarda ise önemli derecede düzelme görüldü. Ek olarak Se ve ACA tedavileri, DOXR'in indüklediği 8-OHdG ekspresyonunu azalttı (p< 0.05). Bu bulgular, Se ve ACA'nın, DOXR kaynaklı böbrek hasarından sonra meydana gelebilecek böbrek fonksiyon bozukluğunu ve oksidatif DNA hasarını baskılamak için önemli terapötik ajanlar olarak kullanılabileceğini gösterdi.

Keywords

8-OHdG, Böbrek, Doksorubisin, N-(p-Amilsinnamoil) antranilik asit, Selenyum.

EFFECT OF SELENIUM AND N-(P-AMYLCINNAMOYL) ANTHRANILIC ACID ON DOXORUBICIN-INDUCED KIDNEY INJURY IN RATS

Abstract

Doxorubicin (DOXR) is one of the essential antitumor drugs. However, its serious adverse effects in many organs limit the clinical use of DOXR. This study aimed to investigate the effect of selenium (Se) and N-(p-Amilcinnamoyl) anthranilic acid (ACA) on kidney tissue in DOXR-administered rats. The rats in the study were divided into six groups (n=10); Control, DMSO, DOXR, DOXR+Se, DOXR+ACA and DOXR+Se+ACA. At the end of the study, intracardiac blood was drawn from rats, and kidney tissues were removed. Urea and creatine levels were measured in serum samples of rats. In addition, histopathological examination of kidney tissue was determined by H&E staining, and 8-OHdG expression was determined by immunohistochemical analysis. Urea and creatine levels increased with DOXR decreased in serum samples after Se and ACA treatments (p< 0.05). While glomerular atrophy, tubular and glomerular dilatation, vascular occlusion and degeneration of tubular epithelial cells were observed in the DOXR group, significant improvement was observed in the Se and ACA treatment groups. In addition, Se and ACA treatments reduced DOXR-induced 8-OHdG expression (p< 0.05). These findings indicated that Se and ACA could be used as critical therapeutic agents to suppress renal dysfunction and oxidative DNA damage that can occur after DOXR-induced kidney injury.

Keywords

8-OHdG, Doxorubicin, Kidney, N-(p-Amylcinnamoyl) anthranilic acid, Selenium.

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