Yeni bir Pichia kudriavzevii FOL-27'nin yapay mide sindiriminde hayatta kalması ve biyoproses uyumluluğu

Year 2024, Volume: 2 Issue: 2, 101 - 108, 27.09.2024

Kübra Yumuk , Özge Can Fatih Ortakcı

Abstract

The goal of this study was to explore Pichia kudriavzevii FOL-27’s: i) survival against artificial gastric acid (AGJ) and artificial bile juice (ABJ), ii) growth kinetics in batch trials (BT) and fed-batch trials (FBT). Survival of FOL-27 as measured by relative cell density ratio (RCDR) against AGJ and ABJ was performed at four different pH-levels (control, 3, 2, 1.5) and ox-bile concentrations (control, 0.2%, 1%, 2%), respectively. Growth kinetics was calculated by periodic measurement of OD600 in BT or in FBT where pH, dissolved oxygen and temperature were controlled at 5.5, 25%, and 30°C, respectively. Also, impact of dissolved oxygen level at 12.5% or 25% were tested against the growth and performance of FOL-27 in FBT using exponential feeding regimen. The doubling-time, maximum specific growth rate, and final cell densities achieved for BT were 101.8 min, 8.202h-1 and 28.7, respectively. FBT at 25% O2 or 12.5% O2 level resulted in doubling-time, maximum specific growth rate, and final cell densities of 90.18 min, 3.95h-1, 22.51 and 88.8 min, 2.83h-1, 26.6, respectively. RCDRs calculated were similar for pH=3 and control vs both were remarkably higher (p<0.05) than pH=1.5 and pH=2 with the last two pH-levels were significantly different (p<0.05) from each other. RCDRs were similar across control, 0.2%, 1%, and 2% ox-bile levels (p>0.05). P. kudriavzevii FOL-27 is a potential probiotic candidate showing resistance against AGJ and ABJ conditions. A remarkable increase in biomass when grown with FBT implies that P. kudriavzevii FOL-27 is compatible to bioprocess development therefore a yeast-based probiotic culture could perhaps be developed using this strain.

Keywords

P.kudriavzevii, probiyotikler, fed-batch, biyoproses

Artificial gastric digestion survival and bioprocess compatibility of a novel Pichia kudriavzevii FOL-27

Abstract

The goal of this study was to explore Pichia kudriavzevii FOL-27’s: i) survival against artificial gastric acid (AGJ) and artificial bile juice (ABJ), ii) growth kinetics in batch trials (BT) and fed-batch trials (FBT). Survival of FOL-27 as measured by relative cell density ratio (RCDR) against AGJ and ABJ was performed at four different pH-levels (control, 3, 2, 1.5) and ox-bile concentrations (control, 0.2%, 1%, 2%), respectively. Growth kinetics was calculated by periodic measurement of OD600 in BT or in FBT where pH, dissolved oxygen and temperature were controlled at 5.5, 25%, and 30°C, respectively. Also, impact of dissolved oxygen level at 12.5% or 25% were tested against the growth and performance of FOL-27 in FBT using exponential feeding regimen. The doubling-time, maximum specific growth rate, and final cell densities achieved for BT were 101.8 min, 8.202h-1 and 28.7, respectively. FBT at 25% O2 or 12.5% O2 level resulted in doubling-time, maximum specific growth rate, and final cell densities of 90.18 min, 3.95h-1, 22.51 and 88.8 min, 2.83h-1, 26.6, respectively. RCDRs calculated were similar for pH=3 and control vs both were remarkably higher (p<0.05) than pH=1.5 and pH=2 with the last two pH-levels were significantly different (p<0.05) from each other. RCDRs were similar across control, 0.2%, 1%, and 2% ox-bile levels (p>0.05). P. kudriavzevii FOL-27 is a potential probiotic candidate showing resistance against AGJ and ABJ conditions. A remarkable increase in biomass when grown with FBT implies that P. kudriavzevii FOL-27 is compatible to bioprocess development therefore a yeast-based probiotic culture could perhaps be developed using this strain.

Keywords

P. kudriavzevii, probiotics, fed-batch, bioprocess, dissolved-oxygen

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