In vitro propagation of endemic plant Centaurea arifolia Boiss. Taxa

Year 2012, Volume: 71 Issue: 2, 121 - 127, 25.12.2012

Elif Yüzbaşıoğlu , Eda Dalyan Mehmet Bona Gül Öz

Abstract

Centaurea L. (Asteraceae) is one of the largest genera in the flora of Turkey. Centaurea arifolia belonging
to the genus Centaurea is considered as DD (Data Deficient) according to IUCN category. There are
no studies identified aiming to propagate this species in vitro. In this study, the seeds were germinated
aseptically in Petri dishes containing growth regulator free Murashige and Skoog half-strength (MS ½)
medium. Leaf explants of six-week-old grown seedlings were cultured on MS medium supplemented
with 1 mg l−1 BAP plus 0.1 mg l−1 NAA and 2 mg l−1 BAP plus 0.2 mg l−1 NAA for indirect regeneration
and after three weeks, actively growing calli appeared and proliferated. Induction of adventitious
shoot regeneration from calli was achieved approximately 150% (MS1) and 120% (MS2), within three
weeks. Regenerated adventitious shoots were best rooted on MS medium containing 1 mg l−1 IBA. This
protocol provides a successful propagation technique through indirect in vitro organogenesis from leaf
segments of C. arifolia.
Keywords: C. arifolia, endemic species, micropropagation

Keywords

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Endemik Centaurea arifolia Boiss. bitkisinin in Vitro üretimi

Abstract

Centaurea L. (Asteraceae) Türkiye florasının en geniş cinslerinden biridir. Centaurea cinsine ait olan Centaurea arifolia IUCN kategorisine göre DD (veri yetersiz) olarak değerlendirilmiştir. Bu tür ile ilgili herhangi bir in vitro propagasyon çalışmasına rastlanmamıştır. Bu çalışmada, tohumlar büyüme düzenleyicisi içermeyen 1\2 Murashige ve Skoog (MS ½) ortamında petri kaplarında aseptik koşullarda çimlendirilmiştir. Altı haftalık fidelerden alınan yaprak eksplantları dolaylı rejenerasyon için 1 mg l−1 BAP + 0.1 mg l−1 NAA ve 2 mg l−1 BAP + 0.2 mg l−1 NAA hormon kombinasyonlarını içeren MS ortamına transfer edilmişlerdir. Üç hafta sonra aktif olarak büyüyen kalluslar elde edilmiştir. Kalluslardan üç hafta sonra adventif gövde rejenerasyonunun teşviki yaklaşık olarak % 150 (MS1) ve % 120 (MS2) oranında başarı elde edilmiştir. Rejenere olmuş adventif gövdeler 1 mg l−1 IBA içeren MS ortamında köklendirilmiştir. Bu yöntem, C. arifolia’nın yaprak segmentlerinden in vitro dolaylı organogenesis aracılığı ile oluşturulmuş başarılı bir üretim tekniği sağlamaktadır.

Keywords

C. arifolia, endemik tür, mikroçoğaltım

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