EXPLORING MITOMIRS IN BREAST CANCER: AN IN-VITRO STUDY OF THEIR EMERGING ROLES

Year 2023, Volume: 86 Issue: 1, 69 - 77, 31.01.2023

Pervin Elvan Tokgün , Ayşe Gaye Tomatır

https://doi.org/10.26650/IUITFD.1200910

Abstract

Objective: Breast cancer is associated with a 5% genetic predisposition in women. Mitochondria play a role in important cellular events such as metabolism, cell death, and inflammation. Recent studies have highlighted precursor micro-RNA (pre-miRNA) to be located in mitochondria as well as mature miRNA. This study aims to reveal the occurrence of mitochondrial miRNA (mitomiR) in breast cancer cells. Materials and Methods: The study has prepared the mitochondrial fractions using the magnetic-activated cell sorting (MACS) method and performed small RNA (sRNA) sequencing. Results: The study has identified known and novel mitomiR sequences aligned to the mitochondrial genome. Conclusion: Identifying new mitomiRs can provide significant contributions and illuminate the molecular mechanism underlying mitomiR biogenesis.

Keywords

mitomiRs, MACS, breast cancer, small RNA sequencing

Supporting Institution

Pamukkale Üniversitesi BAP

Project Number

2014SBE011

Thanks

We want to thank Prof. Dr. Gülseren Bağcı for her laboratory support and scientific comments, Assoc.Prof.Dr. Onur Tokgun for contributing with his valuable scientific support and Msc.Serap Kurt for laboratory support.

MEME KANSERİNDE MİTOMİR’LERİN TANIMLANMASI: İN VİTRO ÇALIŞMA İLE ROLLERİNİN GÖSTERİLMESİ

Abstract

Amaç: Kadınlarda meme kanseri %5 oranında genetik yatkınlıkla ilişkilidir. Mitokondri, metabolizma, hücre ölümü ve inflamasyon gibi önemli hücresel olaylarda rol oynar. Son çalışmalar, olgun miRNA’ların yanı sıra pre-miRNA’ların mitokondride bulunduğunu göstermişlerdir. Bu çalışmada meme kanseri hücrelerinde mitomiR’lerin varlığını ortaya koymayı amaçladık. Gereç ve Yöntem: MACS yöntemi kullanılarak mitokondriyal fraksiyonlar hazırlandı ve küçük RNA dizilimi yapıldı. Bulgular: Mitokondriyal genoma hizalanmış bilinen ve yeni mitomiR dizilerini belirledik. Sonuç: Yeni mitomiR’lerin tanımlanması literatüre önemli katkılar sağlayabilir ve mitomiR biyogenezi moleküler mekanizmalar ile aydınlatılabilir.

Keywords

meme kanseri, mitomiR, MACS, küçük RNA dizileme

Project Number

2014SBE011

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