HOW DO THE GENETIC VARIANTS OF MMP 9 AND TIMP 1 AND VITAMIN D AFFECT CHRONIC RHINOSINUSITIS WITH NASAL POLYPOSIS?

Meltem Bozacı Kılıçoğlu; Baris Ertugrul; Göksu Kaşarcı; Şenol Çomoğlu; Levent Aydemir; Meryem Nesil Keles Türel; A Gelincik; Bedia Çakmakoğlu

doi:10.26650/IUITFD.1210739

Research Article

HOW DO THE GENETIC VARIANTS OF MMP 9 AND TIMP 1 AND VITAMIN D AFFECT CHRONIC RHINOSINUSITIS WITH NASAL POLYPOSIS?

Year 2023, Volume: 86 Issue: 2, 152 - 158, 30.03.2023

Meltem Bozacı Kılıçoğlu , Baris Ertugrul , Göksu Kaşarcı , Şenol Çomoğlu , Levent Aydemir , Meryem Nesil Keles Türel , A Gelincik , Bedia Çakmakoğlu

https://doi.org/10.26650/IUITFD.1210739

Abstract

Objective: Chronic rhinosinusitis with nasal polyposis (CRSwNP) is a multifactorial disease in which the sinus and nasal mucosa are affected. We investigated the relationship between the genetic polymorphism of MMP9 (rs17576) and TIMP1 (rs48498) and serum levels, and we wanted to investigate the effect of MMP9 and TIMP1 genotypes on Vitamin serum levels in CRSwNP with and without Aspirin-Exacerbated Respiratory Disease (AERD). Materials and Methods: This study consisted of 99 patients with CRSwNP with AERD and CRSwNP without AERD. The RTPCR method and ELISA were used in this study. ELISA was used for MMP9 and TIMP1 serum levels and Vitamin D levels. Results: Serum levels of MMP9 were markedly higher in AG genotype than GG genotype in CRSwNP with AERD. Serum levels of MMP9 were statistically higher in carrying of A (+) allele in CRSwNP with AERD than in not carrying of A (+) allele. In addition, serum levels of Vitamin D were discovered significantly lower in TIMP1 CT genotype in comparison to CC and TT genotypes in all of CRSwNP. Conclusion: The rising of MMP9 serum levels may be a marker of chronic inflammation or they may be a significant factor in the resistance to diseases. This study may lead to a further understanding of disease severity in CRSwNP with AERD.

Keywords

Nasal polyposis, MMP9, TIMP1, genetic polymorphism, chronic rhinosinusitis

Supporting Institution

Scientific Research Projects Coordination Unit of Istanbul University

Project Number

TTU-2018-31960

References

1. Fokkens WJ, Lund VJ, Mullol J, Bachert C, Alobid I, Baroody F, et al. European position paper on rhinosinusitis and nasal polyps 2012. Rhinol 2012;23(Suppl):1-298. 14. google scholar
2. Sousa AR, Parikh A, Scadding G, Corrigan CJ, Lee TH. Leukotriene-receptor expression on nasal mucosal inflammatory cells in aspirin-sensitive rhinosinusitis. N Engl J Med 2002;347(19):1493-9. [CrossRef] 15. google scholar
3. Brew K, Dinakarpandian D, Nagase H. Tissue inhibitors of metalloproteinases: evolution, structure, and function. Biochim Biophys Acta 2000;1477(1-2):267-83. [CrossRef] google scholar
4. Wang LF, Lee CH, Chien CY, Chen JY, Chiang FY, Tai CF. 16. Serum 25-hydroxyvitamin D levels are lower in chronic rhinosinusitis with nasal polyposis and are correlated with disease severity in Taiwanese patients. Am J Rhinol Allergy 2013;27(6):e162-5. [CrossRef] google scholar
5. Mulligan JK, White DR, Wang EW, Sansoni SR, Moses H, Yawn 17. RJ. Vitamin D3 deficiency increases sinus mucosa dendritic cells in pediatric chronic rhinosinusitis with nasal polyps. Otolaryngol Head Neck Surg 2012;147(4):773-81. [CrossRef] google scholar
6. Thomas M, Yawn B, Price D, Lund V, Mullol J, Fokkens W.et al. EPOS primary care guidelines: European position 18. paper on the primary care diagnosis and management of rhinosinusitis and nasal polyps 2007 - a summary. Primary Care Respiratory Journal 2008;17(2):79-89. [CrossRef] google scholar
7. Watelet JB, Bachert C, Claeys C, Cauwenberge PV. Matrix 19. metalloproteinases MMP-7, MMP-9, and their tissue inhibitor TIMP-1: expression in chronic sinusitis vs nasal polyposis. Allergy 2004;59(1):54-60. [CrossRef] google scholar
8. Stevens WW, Peters AT, Hirsch AG, Nordberg CM, Schwartz 20. BS, Mercer DG et al. Clinical characteristics of patients with chronic rhinosinusitis with nasal polyps, asthma, and aspirin-exacerbated respiratory disease. J Allergy Clin Immunol Pract 2017;5(4):1061-70. [CrossRef] google scholar
9. Wang LF, Chien CY, Tai CF, Kuo WR, Hsi E, Hank Juo SH. Matrix metalloproteinase-9 gene polymorphisms in nasal polyposis. BMC Med Genet 2010;11:85. [CrossRef] google scholar
10. Guerra G, Testa D, Salzano AF, Tafuri D, Hay E, Schettino A. Expression of matrix metalloproteinases and their tissue inhibitors in chronic rhinosinusitis with nasal polyps: etiopathogenesis and recurrence. Ear Nose Throat J 2021;100(5_Suppl):597S-605S [CrossRef] google scholar
11. Yeo NK, Eom DW, Oh MY, Lim HW, Song YJ. Expression of matrix metalloproteinase 2 and 9 and tissue inhibitor of metalloproteinase 1 in nonrecurrent vs recurrent nasal polyps. Ann Allergy Asthma Immunol 2013;111(3):205-10. [CrossRef] google scholar
12. Mudd PA, Katial RK, Alam R, Hohensee S, Ramakrishnan V, Kingdom TT. Variations in expression of matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 in the nasal mucosa of aspirin-sensitive versus aspirin-tolerant patients with nasal polyposis. Ann Allergy Asthma Immunol 2011;107(4):353-9. [CrossRef] google scholar
13. Lai CY, Chang WS, Hsieh YH, Hsu CM, Tsai CW, Chen AC, et al. Association of tissue inhibitor of metalloproteinase-1 genotypes with lung cancer risk in Taiwan. Anticancer Res 2016;36(1):155-60. google scholar
14. Chang WS, Liu LC, Hsiao CL, Su CH, Wang HC, Ji HX et al. The contributions of the tissue inhibitor of metalloproteinase-1 genotypes to triple-negative breast cancer risk. Biomedicine (Taipei) 2016;6(1):4. [CrossRef] google scholar
15. Wang HX, Yang QD, Liu BQ, Zhang L, Ma MM, Hu ZY, et al. TIMP-1 polymorphisms in a Chinese Han population with intracerebral hemorrhage. Int J Neurosci 2014;124(1):61-7. [CrossRef] google scholar
16. Chotchaeva ZC, Shcheglova EV, Boeva OI, Hait GY, Magazinyuk TP, Rogova SS, et al. Matrix metalloproteinase 9 and tissue inhibitor of matrix metalloproteinases type 1 serum concentrations and genetic polymorphism in senile aortic stenosis. Adv Gerontol 2015;28(2):222-7. [CrossRef] google scholar
17. Vira H, Pradhan V, Umare V, Chaudhary A., Rajadhyksha A., Nadkar M. et al.Role of polymorphisms in MMP-9 and TIMP-1 as biomarkers for susceptibility to systemic lupus erythematosus patients. Birromark Med 2019;13(1):33-43. [CrossRef] google scholar
18. Hashemian F, Sadegh S, Jahanshahi J, Rabiei MAS, Hashemian F. Effects of Vitamin D supplementation on recurrence of nasal polyposis after endoscopic sinus surgery. Iran J. Otorhinolaryngol 2020;32(108):21-28 google scholar
19. Alnori H, Alassaf FA, Alfahad M, Qazzaz ME, Jasim M, Abed MN. Vitamin D and Immunoglobulin E status in allergic rhinitis patients compared to healthy people J Med Life 2020:13(4):463-8. [CrossRef] google scholar
20. Wang LF, Tai CF, Chien CY, Chiang FY, Chen JYF. Vitamin D decreases the secretion of matrix metalloproteinase-2 and matrix metalloproteinase-9 in fibroblasts derived from Taiwanese patients with chronic rhinosinusitis with nasal polyposis. Kaohsiung J Med Sci 2015;31(5):235-40. [CrossRef] google scholar

MMP 9 VE TIMP 1 GENETİK VARYANTLARI VE VİTAMİN D NAZAL POLİPOZİSLİ KRONİK SİNÜZİTTE NASIL ETKİLİDİR?

Year 2023, Volume: 86 Issue: 2, 152 - 158, 30.03.2023

Meltem Bozacı Kılıçoğlu , Baris Ertugrul , Göksu Kaşarcı , Şenol Çomoğlu , Levent Aydemir , Meryem Nesil Keles Türel , A Gelincik , Bedia Çakmakoğlu

https://doi.org/10.26650/IUITFD.1210739

Abstract

Amaç: Nazal polipli kronik rinosinüzit sinüs ve nazal mukozanın etkilendiği multifaktöriyel bir hastalıktır. MMP9 (rs17576) ve TIMP1 (rs 4898) genetik polimorfizmleri ile serum düzeyi arasındaki ilişki ve MMP9 ve TIMP1 genotipleri ve Vitamin D arasındaki bağlantının gösterilmesini araştırdık. Gereç ve Yöntem: Bu çalışma, aspirinle alevlenen solunum hastalığı olan veya olmayan nazal polipozisli kronik rinosinüzitli 99 hastadan oluşmaktadır. RT-PCR ve ELISA metodu kullanılmıştır. MMP9, TIMP1 ve Vitamin D serum seviyeleri için ELISA metodu kullanılmıştır. Bulgular: Aspirinle alevlenen solunum hastalığı eşlik eden nazal polipozisli kronik sinüzit hastalarında MMP9 serum seviyesi, AG genotipli hastalarda GG genotipli hastalara kıyasla daha yüksek seviyededir. Aynı zamanda bu hasta grubunda MMP9 serum seviyesi, A (+) alleli taşıyan grupta A (+) alleli taşımayan gruba göre daha yüksek seviyededir. Buna ek olarak TIMP1 CT genotipine sahip olanlar, TIMP1 CC ve TT genotipleri ile kıyaslandığında Vitamin D serum seviyesinin anlamlı olarak düşük olduğu gösterilmiştir. Sonuç: MMP9 serum düzeylerinin yükselmesi, kronik inflamasyonun bir belirteci olabilir veya hastalık direncinde önemli bir faktör olabilir. Bu çalışma, aspirinle alevlenen solunum hastalığı olan nazal polipozisli kronik rinosinüzitte hastalık şiddetinin daha iyi anlaşılmasına yardımcı olabilir.

Keywords

Nazal polip, MMP9, TIMP1, genetik polimorfizm, kronik sinüzit

Project Number

TTU-2018-31960

References

1. Fokkens WJ, Lund VJ, Mullol J, Bachert C, Alobid I, Baroody F, et al. European position paper on rhinosinusitis and nasal polyps 2012. Rhinol 2012;23(Suppl):1-298. 14. google scholar
2. Sousa AR, Parikh A, Scadding G, Corrigan CJ, Lee TH. Leukotriene-receptor expression on nasal mucosal inflammatory cells in aspirin-sensitive rhinosinusitis. N Engl J Med 2002;347(19):1493-9. [CrossRef] 15. google scholar
3. Brew K, Dinakarpandian D, Nagase H. Tissue inhibitors of metalloproteinases: evolution, structure, and function. Biochim Biophys Acta 2000;1477(1-2):267-83. [CrossRef] google scholar
4. Wang LF, Lee CH, Chien CY, Chen JY, Chiang FY, Tai CF. 16. Serum 25-hydroxyvitamin D levels are lower in chronic rhinosinusitis with nasal polyposis and are correlated with disease severity in Taiwanese patients. Am J Rhinol Allergy 2013;27(6):e162-5. [CrossRef] google scholar
5. Mulligan JK, White DR, Wang EW, Sansoni SR, Moses H, Yawn 17. RJ. Vitamin D3 deficiency increases sinus mucosa dendritic cells in pediatric chronic rhinosinusitis with nasal polyps. Otolaryngol Head Neck Surg 2012;147(4):773-81. [CrossRef] google scholar
6. Thomas M, Yawn B, Price D, Lund V, Mullol J, Fokkens W.et al. EPOS primary care guidelines: European position 18. paper on the primary care diagnosis and management of rhinosinusitis and nasal polyps 2007 - a summary. Primary Care Respiratory Journal 2008;17(2):79-89. [CrossRef] google scholar
7. Watelet JB, Bachert C, Claeys C, Cauwenberge PV. Matrix 19. metalloproteinases MMP-7, MMP-9, and their tissue inhibitor TIMP-1: expression in chronic sinusitis vs nasal polyposis. Allergy 2004;59(1):54-60. [CrossRef] google scholar
8. Stevens WW, Peters AT, Hirsch AG, Nordberg CM, Schwartz 20. BS, Mercer DG et al. Clinical characteristics of patients with chronic rhinosinusitis with nasal polyps, asthma, and aspirin-exacerbated respiratory disease. J Allergy Clin Immunol Pract 2017;5(4):1061-70. [CrossRef] google scholar
9. Wang LF, Chien CY, Tai CF, Kuo WR, Hsi E, Hank Juo SH. Matrix metalloproteinase-9 gene polymorphisms in nasal polyposis. BMC Med Genet 2010;11:85. [CrossRef] google scholar
10. Guerra G, Testa D, Salzano AF, Tafuri D, Hay E, Schettino A. Expression of matrix metalloproteinases and their tissue inhibitors in chronic rhinosinusitis with nasal polyps: etiopathogenesis and recurrence. Ear Nose Throat J 2021;100(5_Suppl):597S-605S [CrossRef] google scholar
11. Yeo NK, Eom DW, Oh MY, Lim HW, Song YJ. Expression of matrix metalloproteinase 2 and 9 and tissue inhibitor of metalloproteinase 1 in nonrecurrent vs recurrent nasal polyps. Ann Allergy Asthma Immunol 2013;111(3):205-10. [CrossRef] google scholar
12. Mudd PA, Katial RK, Alam R, Hohensee S, Ramakrishnan V, Kingdom TT. Variations in expression of matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 in the nasal mucosa of aspirin-sensitive versus aspirin-tolerant patients with nasal polyposis. Ann Allergy Asthma Immunol 2011;107(4):353-9. [CrossRef] google scholar
13. Lai CY, Chang WS, Hsieh YH, Hsu CM, Tsai CW, Chen AC, et al. Association of tissue inhibitor of metalloproteinase-1 genotypes with lung cancer risk in Taiwan. Anticancer Res 2016;36(1):155-60. google scholar
14. Chang WS, Liu LC, Hsiao CL, Su CH, Wang HC, Ji HX et al. The contributions of the tissue inhibitor of metalloproteinase-1 genotypes to triple-negative breast cancer risk. Biomedicine (Taipei) 2016;6(1):4. [CrossRef] google scholar
15. Wang HX, Yang QD, Liu BQ, Zhang L, Ma MM, Hu ZY, et al. TIMP-1 polymorphisms in a Chinese Han population with intracerebral hemorrhage. Int J Neurosci 2014;124(1):61-7. [CrossRef] google scholar
16. Chotchaeva ZC, Shcheglova EV, Boeva OI, Hait GY, Magazinyuk TP, Rogova SS, et al. Matrix metalloproteinase 9 and tissue inhibitor of matrix metalloproteinases type 1 serum concentrations and genetic polymorphism in senile aortic stenosis. Adv Gerontol 2015;28(2):222-7. [CrossRef] google scholar
17. Vira H, Pradhan V, Umare V, Chaudhary A., Rajadhyksha A., Nadkar M. et al.Role of polymorphisms in MMP-9 and TIMP-1 as biomarkers for susceptibility to systemic lupus erythematosus patients. Birromark Med 2019;13(1):33-43. [CrossRef] google scholar
18. Hashemian F, Sadegh S, Jahanshahi J, Rabiei MAS, Hashemian F. Effects of Vitamin D supplementation on recurrence of nasal polyposis after endoscopic sinus surgery. Iran J. Otorhinolaryngol 2020;32(108):21-28 google scholar
19. Alnori H, Alassaf FA, Alfahad M, Qazzaz ME, Jasim M, Abed MN. Vitamin D and Immunoglobulin E status in allergic rhinitis patients compared to healthy people J Med Life 2020:13(4):463-8. [CrossRef] google scholar
20. Wang LF, Tai CF, Chien CY, Chiang FY, Chen JYF. Vitamin D decreases the secretion of matrix metalloproteinase-2 and matrix metalloproteinase-9 in fibroblasts derived from Taiwanese patients with chronic rhinosinusitis with nasal polyposis. Kaohsiung J Med Sci 2015;31(5):235-40. [CrossRef] google scholar

There are 20 citations in total.

Details

Primary Language	English
Subjects	Health Care Administration
Journal Section	RESEARCH
Authors	Meltem Bozacı Kılıçoğlu 0000-0001-9952-0329 Baris Ertugrul 0000-0003-3878-1829 Göksu Kaşarcı 0000-0001-9766-4361 Şenol Çomoğlu 0000-0003-4632-9218 Levent Aydemir 0000-0002-5836-4304 Meryem Nesil Keles Türel 0000-0003-1829-8186 A Gelincik 0000-0002-3524-9952 Bedia Çakmakoğlu 0000-0001-7960-9131
Project Number	TTU-2018-31960
Publication Date	March 30, 2023
Submission Date	November 29, 2022
Published in Issue	Year 2023 Volume: 86 Issue: 2

Cite

APA	Bozacı Kılıçoğlu, M., Ertugrul, B., Kaşarcı, G., Çomoğlu, Ş., et al. (2023). HOW DO THE GENETIC VARIANTS OF MMP 9 AND TIMP 1 AND VITAMIN D AFFECT CHRONIC RHINOSINUSITIS WITH NASAL POLYPOSIS?. Journal of Istanbul Faculty of Medicine, 86(2), 152-158. https://doi.org/10.26650/IUITFD.1210739
AMA	Bozacı Kılıçoğlu M, Ertugrul B, Kaşarcı G, Çomoğlu Ş, Aydemir L, Keles Türel MN, Gelincik A, Çakmakoğlu B. HOW DO THE GENETIC VARIANTS OF MMP 9 AND TIMP 1 AND VITAMIN D AFFECT CHRONIC RHINOSINUSITIS WITH NASAL POLYPOSIS?. İst Tıp Fak Derg. March 2023;86(2):152-158. doi:10.26650/IUITFD.1210739
Chicago	Bozacı Kılıçoğlu, Meltem, Baris Ertugrul, Göksu Kaşarcı, Şenol Çomoğlu, Levent Aydemir, Meryem Nesil Keles Türel, A Gelincik, and Bedia Çakmakoğlu. “HOW DO THE GENETIC VARIANTS OF MMP 9 AND TIMP 1 AND VITAMIN D AFFECT CHRONIC RHINOSINUSITIS WITH NASAL POLYPOSIS?”. Journal of Istanbul Faculty of Medicine 86, no. 2 (March 2023): 152-58. https://doi.org/10.26650/IUITFD.1210739.
EndNote	Bozacı Kılıçoğlu M, Ertugrul B, Kaşarcı G, Çomoğlu Ş, Aydemir L, Keles Türel MN, Gelincik A, Çakmakoğlu B (March 1, 2023) HOW DO THE GENETIC VARIANTS OF MMP 9 AND TIMP 1 AND VITAMIN D AFFECT CHRONIC RHINOSINUSITIS WITH NASAL POLYPOSIS?. Journal of Istanbul Faculty of Medicine 86 2 152–158.
IEEE	M. Bozacı Kılıçoğlu, B. Ertugrul, G. Kaşarcı, Ş. Çomoğlu, L. Aydemir, M. N. Keles Türel, A. Gelincik, and B. Çakmakoğlu, “HOW DO THE GENETIC VARIANTS OF MMP 9 AND TIMP 1 AND VITAMIN D AFFECT CHRONIC RHINOSINUSITIS WITH NASAL POLYPOSIS?”, İst Tıp Fak Derg, vol. 86, no. 2, pp. 152–158, 2023, doi: 10.26650/IUITFD.1210739.
ISNAD	Bozacı Kılıçoğlu, Meltem et al. “HOW DO THE GENETIC VARIANTS OF MMP 9 AND TIMP 1 AND VITAMIN D AFFECT CHRONIC RHINOSINUSITIS WITH NASAL POLYPOSIS?”. Journal of Istanbul Faculty of Medicine 86/2 (March 2023), 152-158. https://doi.org/10.26650/IUITFD.1210739.
JAMA	Bozacı Kılıçoğlu M, Ertugrul B, Kaşarcı G, Çomoğlu Ş, Aydemir L, Keles Türel MN, Gelincik A, Çakmakoğlu B. HOW DO THE GENETIC VARIANTS OF MMP 9 AND TIMP 1 AND VITAMIN D AFFECT CHRONIC RHINOSINUSITIS WITH NASAL POLYPOSIS?. İst Tıp Fak Derg. 2023;86:152–158.
MLA	Bozacı Kılıçoğlu, Meltem et al. “HOW DO THE GENETIC VARIANTS OF MMP 9 AND TIMP 1 AND VITAMIN D AFFECT CHRONIC RHINOSINUSITIS WITH NASAL POLYPOSIS?”. Journal of Istanbul Faculty of Medicine, vol. 86, no. 2, 2023, pp. 152-8, doi:10.26650/IUITFD.1210739.
Vancouver	Bozacı Kılıçoğlu M, Ertugrul B, Kaşarcı G, Çomoğlu Ş, Aydemir L, Keles Türel MN, Gelincik A, Çakmakoğlu B. HOW DO THE GENETIC VARIANTS OF MMP 9 AND TIMP 1 AND VITAMIN D AFFECT CHRONIC RHINOSINUSITIS WITH NASAL POLYPOSIS?. İst Tıp Fak Derg. 2023;86(2):152-8.

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