Anticancer activities and cell death mechanisms of 1H-indole-2,3-dione 3-[N-(4 sulfamoylphenyl)thiosemicarbazone] derivatives

Year 2018, Volume: 48 Issue: 3, 63 - 67, 01.12.2018

İdil Çetin , Pınar Eraslan Elma Mehmet Topçul , Nilgün Karalı

Abstract

DOI: 10.26650/IstanbulJPharm.2018.414805

In this study, the cytotoxic effects of
1H-indole-2,3-dione 3-[N-(4-sulfamoylphenyl)thiosemicarbazone derivatives
namely, 4a-d were evaluated using cell kinetic parameters including the cell
index, mitotic index, labelling index and apoptotic index on HeLa cells taken
from a human cervix carcinoma. All compounds were evaluated using cell index
parameters at 5, 10, 20, 40, 80, 100 and 160 μM concentrations. As a result of
this, it was seen that all 4a-d compounds were effective in different
concentrations. Different cell death mechanisms were proposed for 4a-d. When
all the parameters were examined, it was found that the bromine substituted 4c
was the most potent antiproliferative compound in the tested compounds. The
difference was significant between the control and experimental groups
(p<0.01). In addition, a statistically significant difference was noted
among all experimental groups (p<0.01).

Keywords

1H-indole-2, 3-dione, thiosemicarbazone, antiproliferative effect, HeLa cells

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