Aromatherapeutic essential oils and their pharmaceutical combinations: Tools for inhibition of quorum sensing activity and biofilm formation of human pathogens*

Year 2022, Volume: 52 Issue: 1, 54 - 63, 28.04.2022

Müjgan Özfenerci Elif Burcu Bali , Demet Erdönmez , Ufuk Koca Çalışkan

https://doi.org/10.26650/IstanbulJPharm.2022.998774

Abstract

Background and Aims: Aromatherapy, as one of the complementary therapies, uses essential oils as the main therapeutic agents to treat several diseases. In the present study, it was aimed to investigate inhibition of quorum sensing (QS) and bio- film formation of aromatherapeutic essential oils (AEOs) and their pharmaceutical combinations (PC-I and PC-II).
Methods: The anti-QS potential of AEOs were determined using the biosensor strains Chromobacterium violaceum ATCC 12472 and Pseudomonas aeruginosa PAO1. Anti-QS activity was detected by agar-well diffusion and violacein pigment inhi- bition assays. Blocking of PAO1 swim and swarm motilities and biofilm formation was also performed.
Results: Most of the AEOs demonstrated highly active (>95%) violacein pigment inhibition. Additionally, they inhibited swarming (40.34%-72.80%) and swimming (20.06%-50.08%) motilities of PAO1. Moreover, the majority of AEOs also de- creased the biofilm formation, particularly on P. aeruginosa and S. aureus.
Conclusion: Consequently, aromatherapeutic formulations might be a complementary or prophylactic cure for infectious dis- ease by their anti-QS and antibiofilm activities rather than just antimicrobial effects.

Keywords

Aromatherapeutic essential oils, Antibiofilm, Quorum sensing, Pseudomonas aeruginosa PAO1, Synergistic effect

Supporting Institution

Gazi Üniversitesi

Project Number

02/2019-12

Thanks

This work was performed as a part of Müjgan Özfenerci’s Master of Science thesis and supported by the Scientific Research Projects Unit of Gazi University, Ankara-Turkey Grant number: 02/2019-12.

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