Quantification of MMP-2 and TIMP-1 expressions in breast cancer

Year 2023, Volume: 53 Issue: 2, 211 - 218, 30.08.2023

Seda Eren Keskin , Deniz Sünnetçi Akkoyunlu , Mehtap Yılmaz , Turgay Şimşek , Sertaç Güler , Naci Çine , Zafer Cantürk , Hakan Savlı

https://doi.org/10.26650/IstanbulJPharm.2023.1053089

Abstract

Background and Aims: MMP-2 and TIMP-1 are vital molecules in the remodeling of the extracellular matrix, and they have a critical role in the metastatic process of breast cancer. This study aimed to detect expression levels of MMP-2 and TIMP-1 genes by Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) and identify their potential roles in breast cancer prognosis.
Methods: MMP-2 and TIMP-1 gene expression levels in 17 breast cancer tumor tissues and normal breast tissue were examined. The expression levels of MMP-2 and TIMP-1 were analyzed by qRT-PCR. The association between the expression levels of MMP-2 and TIMP-1 and clinicopathological manifestations of breast cancer was examined.
Results: Lower gene expression levels of MMP-2 and TIMP-1 were detected in tumors compared to the controls. A statistical correlation was not observed between the expression level of MMP-2, TIMP-1, and clinicopathological parameters (tumor grade, lymph node involvement, hormone receptor status).
Conclusion: Our findings have been suggesting that expression profiles of MMP-2 and TIMP-1 might be independent prognostic and predictive biomarkers for breast cancer. These biomarkers are candidate molecules for personalized therapy. MMP-2 and TIMP-1 expression patterns will also aid in the identification of more precise and targeted subgroups of breast cancer.

Keywords

Breast cancer, MMP2 metalloproteinase, TIMP-1

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