Domuzlarda Mycoplasma hyopneumoniae İnfeksiyonunun (Domuzların Enzootik Pnömonisi) Bakteriyolojik ve Serolojik Olarak Araştırılması

Year 2011, Volume: 37 Issue: 1, 9 - 16, 31.10.2011

Sedat Çayukli N. Yakut Özgür

Abstract

Bu çalışmada, Domuz Enzootik Pnömonisi' ne neden olan Mycoplasma hyopneumoniae (M. hyopneumoniae)' nin Türkiye'deki varlığı bakteriyolojik ve serolojik olarak araştırıldı. Bakteriyolojik inceleme için, Türkiye' de tek yasal domuz üretimi yapılan Kırklareli yöresindeki bir çiftlikte yetiştirilen ve İstanbul' da kesime sevk edilen 102 domuzdan kesim sırasında akciğer örnekleri alındı. Hastalığın seroprevalansının saptanması amacıyla, akciğer örnekleri alınan domuzlar da dahil olmak üzere, farklı yörelerde yetiştirilen 384 domuz kan serumu örnekleri ELISA ile incelendi. Bakteriyolojik inceleme sonucunda 102 akciğer örneğinden M. hyopneumoniae izole edilmedi. ELISA ile incelenen 384 domuz serumundan 214' ünde spesifik M. hyopneuominae aktikorları saptandı ve hastalığın seroprevalansı % 55,73 olarak belirlendi.

Anahtar Kelimeler: M. hyopneumoniae, enzootik pnömoni, kültür, seroloji, ELISA

Keywords

M. hyopneumoniae, enzootik pnömoni, kültür, seroloji, ELISA

References

• Asai, T., Okada, M., Yokomizo, Y . , Sato, S., Mori, Y., 1996. Supressive effect of bronchoalveolar lavage flu,d from pigs infected with M. hyopneuomonie on chemilumiescence of porcine peripheral neutrophils. Veterinary Immunolgy and Immunopathology 51, 325 - 331.
• Assunçao, P., De L a Fe, C., Kokotovic, B., Gonzalez, O., Poveda, J . B., 2005. The Occurence of mycoplasmas in the lungs of swine in Gran Canaria (Spain). Veterinary Research Communications 29, 453 - 462.
• Bağcıgil, A. F . , İkiz S., Özgür N. Y . , 2009. Detection of Mycoplasma hyopneumoniae in pigs in Turkey. Turkish Journal of Veterinary & Animal Science 33, 61 - 65.
• Bargen, L . E . , 2004 A system response to an outbreak of enzootic pneumoniae in grow / finish pigs. Canadian Veterinary Journal 45, 856 - 859.
• Baumeister, A. K., Runge, M., Ganter, M., Feenstra, A. A., Delbeck, F . , Kirchhoff, H., 1998. Detection Mycoplasma hyopneumoniae in bronchoalveolar lavage fluids of pigs by PCR. Journal of Clinical Microbiology 36, 1984 - 1988.
• Bereiter, M., Young, T. F . , Joo, H. S., Ross, R. F . , 1990. Evaluation of the ELISA and comparison to the complement fixation test and radial immunodiffusion enzyme assay for detection of antibodies against Mycoplasma hyopneumoniae in swine serum. Veterinary Microbiology 25, 177 - 192.
• Betts, A. O. 1971. The role of mycoplasmas in diseases of pigs. Proceedings of the Royal Society Medicine 64, 34 - 35.
• Caron, J . , Ourdani, M., Dea, S., 2000. Diagnosis and differentiation of Mycoplasma hyopneumoniae and Mycoplasma hyorhinis infections in pigs PCR amplification of the p36 and p46 genes. Journal of Clinical Microbiology 38, 1959 - 1966.
• Dubosson, C . R., Conzelmann, C . , Miserez, R., Boerlin, P., Frey, J . , Zimmermann, W., Hani, H., Kuhnert, P., 2004. Developement of two real time PCR assays the detection of Mycoplasma hyopneumoniae in clinical samples. Veterinary Microbiology 102, 55 - 65.
• Falk, K., Hoie, S., Lium, B. M., 1991. An abattoir survey of pneumoniae and pleuritis in slaughter weight swine from nine selected herds. I I . Enzootic pneumoniae of pigs: microbiological findings and their relationship to pathomorphology. Acta Veterinaria Scandinavica 32, 67 - 77.
• Furlong, S. L . , Turner, A. J . , 1975. Isolation of Mycoplasma hyopneumoniae and its association with pneumoniae of pigs in Australia. Australian Veterinary Journal 51, 28 - 31.
• Hege, R., Zimmermann, W., Scheidegger, R., Stark, K. D. C . , 2002. Incidence of reinfections with Mycoplasma hyopneumoniae and Actinobacillus pleuropneumoniae in pig farms located in respiratory disease free regions of Switzerland. Acta Veterinaria Scandinavica 43, 145 - 156.
• Kazama, S., Yagihashi, T., Seto, K., 1989. Preparation of Mycoplasma hyopneumoniae antigen for the Enzyme - Linked Immunosorbent Assay. Canadian Journal of Veterinary Research 53, 176 - 181.
• Kobisch, M., Friis, N. F . , 1996. Swine mycoplasmoses. Scientific and Technical Review 15, 1569 - 1605.
• Le Potier, M. F . , Abiven, P., Kobisch, M., 1994. A blocking ELISA using monoclonal antibody for the serological detection of Mycoplasma hyopneumoniae. Research in Veterinary Science 56, 338 - 345.
• Leon, E . A., Madec, F., Taylor, N. M., Kobisch, M., 2001. Seroepidemiology of Mycoplasma hyopneumoniae in pigs from farrow to finish farms. Veterinary Microbiology 78, 331 - 341.
• Lin, J . H., Chen, S. P., Yeh, K S., Weng, C. N., 2006. Mycoplasma hyorhinis in Taiwan: Diagnosis and isolation of swine pneumoniae pathogen. Veterinary Microbiology 115, 111 - 116.
• Mattsson, J . G., Bergstrom, K., Wallgren, P., Johansson, K., 1995. Detection of Mycoplasma hyopneumoniae in nose swabs from pigs by in vitro amplification of the 16S RNA gene. Journal of Clinical Microbiology 33, 893 - 897.
• Miles, R., Nicholas, R. 1998. Mycoplasma Protocols. Humana Press, U. S. A
• Morris, C. R., Gardner, I. A., Hietala, S. K., Carpenter, T. E . , Anderson, R. J . , Parker, K. M., 1995. Seroepidemiologic study of natural tarnsmission of Mycoplasma hyopneumoniae in a swine herd. Preventive Veterinary Medicine 21, 323 - 337.
• Morrison, R B., Pijoan, H. D., Rapp, V., 1985. Microorganisms associated with pneumoniae in slaughter weight swine. Canadian Journal of Comparative Medicine 49, 129 - 137.
• Otagiri, Y . , Asai, T., Okada, M., Uto, T., Yazawa, S., Hirai, H., Shibata, I . , Sato, S., 2005. Detection of Mycoplasma hyopneumoniae in lung and nasal swab samples from pigs by nasted PCR and culture methods. Journal of Veterinary Science 67, 801 - 805.
• Özdamar, K., 1999. SPSS ile Biyoistatistik. Kaan Kitabevi. Eskişehir - Türkiye.
• Rautianien, E . , Tuovinen, V., Levonen, K., 2000. Monitoring antibodies to Mycoplasma hyopneumoniae in sow colostrum a tool to document freedom of infection. Acta Veterinaria Scandinavica 41, 213 - 225.
• Ross, P. C. 1995. Swine Diseases. Iowa State University Press, Ames - Iowa.
• Scarman, A. L . , James, C. C., Greame, J . E . , Stephen, F. D., Djordjevic, S. P., 1997. Identification of novel species specific antigens of Mycoplasma hyopneumoniae by preparative SDS - PAGE ELISA profiling. Microbiology 143, 663 - 673.
• Senterfit, L . B., 1984. Laboratory Diagnosis of Mycoplasma Infections. Israel Journal of Medical Sciences 20, 905 - 907.
• Sibila, M., Calsamigila, M., Vidal, D., Badiella, L . , Aldaz, A., Jensen, J . C., 2004. Dynamics of Mycoplasma hyopneumoniae infection in 12 farms with different production systems. Canadian Journal of Veterinary Research 68, 12 - 18.
• Tiong, S. K., Sing, K Y . , 1981. Isolation and identification of mycoplasmas from pig lungs in Singapore. Veterinary Research 108, 75 - 77.
• Türkaslan, J . , 1989. Mikoplazma kültürlerinin üreme inhibisyon testi ile identifikasyonu. Pendik Hayvan Hastanesi Merkez Araştırma Enstitüsü Dergisi 2, 60 - 64.
• Van Till, L . D., Dohoo, I. R., Morley, R S., 1991. Epidemiological associations between Mycoplasma hyopenumoniae and Actinobacillus pleuropneumoniae antibody titers and lung lesions in Prince Edward Island swine herds. Canadian Journal of Veterinary Reseach 55, 347 - 351.
• Vicca, J . , Maes, D., Thermote, L . , Peeters, J . , Haesbrouck, F., Kruif, A. D., 2002. Patterns of Mycoplasma hyopneuomiae infections in Belgian farrow to finish pig herds with divering disease course. Journal of Veterinary Medicine 49, 349 - 353.
• Vicca, J . , Stakenborg, T., Maes D., Butaye, P., Peeters, J . , Kruif, A. D., Haesebrouck, F . , 2003. Evaluation of virulence of Mycoplasma hyopneumoniae field isolates. Veterinary Microbiology 97, 177 - 190.