KOYUNDA KAS GELİŞİMİ ÜZERİNE BİR ÇALIŞMA

Year 2012, Volume: 38 Issue: 2, 97 - 106, 14.11.2012

Berjan Demirtaş , Mukaddes Özcan

Abstract

Koyunda kas ontojenisi üzerine çalışıldı. Gebelik yaşları (gy) 48 ile 125 arasında değişen 23 adet tekiz fötus
mezbahadan toplandı. Fötusların ağırlıkları ve baş-kuyruk mesafeleri ölçülerek gebelik yaşları tahmin edildi.
Semitendinosus kası (ST) veya çok küçük olan fötuslardan tüm arka bacak disekte edilerek alkali- adenozin trifosfataz
(ATPaz) ve yavaş-miyozin ağır zincir (MHC) antikoru ile boyandı. Ayrıca, kesitler elektron mikroskopta (EM)
incelendi. Histokimyasal, immunohistokimyasal ve EM çalışmalarına dayanarak kalitatif bulgular elde edildi.
Sonuçlar, bazı sekonder liflerin çok erken dönemde yavaş MHC ekspresyonu yaptıklarını gösterdi. Büyük, yavaş
MHC eksprese eden primer liflerin çevresinde oluşan bu küçük, yavaş MHC ekspresyonu yapan sekonder lifler 52.
günde oluşmaya başlar; yaklaşık 69. günde çapları iyice artar ve yaklaşık 87. günde göç ederek ikinci generasyon
sekonder liflerin (tersiyer) oluşumu için çerçeve görevi görürler. Gelişmiş geç dönem koyun fötusunda görülen büyük,
merkezi ve yavaş MHC eksprese eden (alkali ATPaz ile düşük yoğunlukta da boyanan) liflerin bir kısmının aslında
sekonder lifler olabileceği sonucuna varıldı.

Keywords

Kas, koyun, ontojeni, miyogenez

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