ERİTİLMİŞ KOÇ SPERMASINDA FARKLI GLİSEROL KATMA TEKNİKLERİNİN VE SOĞUTMA HIZININ SPERMATOLOJİK ÖZELLİKLERE ETKİSİ

Year 2007, Volume: 33 Issue: 1, 11 - 21, 26.11.2012

Süleyman Bacınoğlu Ümit Cirit Zakeriya Nur Kemal Ak

Abstract

Sunulan çalışmada spermaya gliserolün farklı ısılarda eklenmesi ve spermanın farklı hızlarda
soğutulmasının, koçlarda donma sonrası spermatolojik özelliklere nasıl etkilediği araştırıldı. Üç-beş yaşlarında
10 baş Kıvırcık ırkı koçun spermaları alınarak pooling yapıldı ve sulandırılarak payetlerde donduruldu.
Spermanın tek aşamada (30°C) veya iki aşamada (30°C’de ve 5°C’de) sulandırılması ve 5°C’ye yavaş
( < 0.2°C/dakika) ve iki-kademeli (30°C’den 16°C’ye < 2°C/dakika ve 16°C’den 5°C’ye < 0.2/dakika) soğutma
tekniklerine göre 6 grup oluşturuldu: Sperma 30°C’de gliserol içermeyen sulandırıcı A ile sulandırıldı ve
5°C’ye yavaş (1. grup) ve iki-kademeli (2. grup) soğutuldu ve B sulandırıcısı ilave edildi. Yine sperma
30°C’de %2 gliserol içeren sulandırıcı A ile sulandırıldı ve 5°C’ye yavaş (3. grup) ve iki-kademeli (4. grup)
soğutuldu. Diğer gruplarda, sulandırma işlemi sulandırıcı A ve B ile 30°C’de tamamlandı ve 5°C’ye yavaş (5.
grup) ve iki-kademeli (6. grup) soğutuldu. Örnekler, ekilibrasyon sonrası payetlerde donduruldu.
Spermatolojik incelemeler (motilite, akrozomal ve toplam morfolojik bozukluklar), sulandırma sonrası (1.
aşama), 5°C’ ye soğutma sonrası (2. aşama), ekilibrasyon sonrası (3. aşama) ve eritme sonrası (4. aşama)
gerçekleştirildi.
Sunulan çalışmada (1) 1:1/2 sulandırma oranında 30°C’den 5°C’ye soğuma aşamasında ortamda
gliserol varlığının, eritme sonrası motiliteye zarar verdiği, (2) bu zararın iki-kademeli soğutma tekniği ile
azaldığı sonuçlarına varılmıştır.

Keywords

Koç Sperması, Soğutma Hızı, Sulandırma Tekniği, Gliserol

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