Detection of the Presence of Bartonella henselae in Cats in Istanbul

Year 2013, Volume: 39 Issue: 2, 209 - 217, 10.03.2013

Belgi Diren Sığırcı , Atila Ilgaz

Abstract

ABSTRACT

In the present study, the determination of the presence of Bartonella henselae in Istanbul in 96 pet, shelter and stray cats were aimed. The samples from the cats were examined by two different bacteriologic culture methods. The blood samples collected into pediatric lysis isolator tubes were spun and the pellet inoculated onto Heart Infusion Agar supplemented with 5% rabbit blood. BACTEC Peds Plus/F were inoculated with samples and incubated in the BACTEC 9050 automated blood culture systems. After the incubation time, growth were evaluated according to the colony morphology, growth time, gram properties and biochemical properties, and it was determined that 27 isolates by solid medium, 10 isolates by BACTEC were Bartonella spp. Isolates were determined as Bartonella henselae by PCR amplification using specific primers of a portion of 16S-23S rRNA intergenic region. In conclusion, the prevalence of Bartonella henselae bacteremia was found 28.1% of cats in Istanbul.

Key Words: Bartonella henselae, cat, isolation, BACTEC, PCR

 

 

 

ÖZET

 

İSTANBUL'DA KEDİLERDE BARTONELLA HENSELAE VARLIĞININ SAPTANMASI

Bu çalışmada, İstanbul ilinde evlerde, barınakta beslenen ve sokakta bulunan 96 adet kedide Bartonella henselae varlığının saptanması amaçlandı. Kedilerden alınan kan örnekleri iki farklı bakteriyolojik kültür yöntemi kullanılarak incelendi. Pediatrik lizis izolatör tüplere alınan kanlar santrifüje edildikten sonra %5 tavşan kanı katkılı Heart Infusion Agarlara ekildi. BACTEC peds/plus F şişelerine alınan kanlar BACTEC 9050 otomatik üreme kontrollü kan kültür sistemi ile inkube edildi. İnkubasyon sonrası üremeler koloni morfolojisi, inkübasyon süresi, Gram özelliği ve biyokimyasal özelliklerine göre değerlendirildi ve izolatör tüplere alınan kanlardan Heart Infusion Agar'da üreyen 27 izolatın, BACTEC şişelerindeki kültürlerde üreyen 10 izolatın Bartonella spp. olduğu belirlendi. İzolatlar 16S-23S rRNA interjenik bölge sekanslarından elde edilen spesifik primerler kullanılarak tür düzeyinde identifiye edildi ve tüm izolatlar Bartonella henselae olarak belirlendi. Bu sonuçlar ile İstanbul yöresindeki kedilerde Bartonella henselae bakteriyemi prevalansı %28,1 olarak ortaya konulmuştur.

Anahtar Kelimeler: Bartonella henselae, kedi, izolasyon, BACTEC, PCR

 

Keywords

Bartonella henselae, kedi, izolasyon, BACTEC, PCR

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