MICROSATELLITE AND ALLOZYME VARIATIONS IN STARLET STURGEON WILD BROODSTOCK AND HATCHERY-PRODUCED OFFSPRING, USED FOR RESTOCKING OF LOWER DANUBE RIVER

Year 2017, Volume: 3 Issue: 4, 199 - 206, 30.09.2017

Petya P. Ivanova , Nina S. Dzhembekova Velichka Kardjeva Angel G. Tsekov Violin S. Raykov

https://doi.org/10.3153/JAEFR17022

Abstract

The natural
population of all sturgeon species has been seriously affected by
overexploitation in combination with a substantial loss and degradation of
habitats during the 20^th century. Fish species undergoing to
restocking require information about the genetic identity of the existing fish
populations. The genetic pattern of sterlet (broodstock and offspring) were
analyzed, using allozymes and seven microsatellite loci (LS19, LS34, LS39,
LS54, LS68, Aox45 and Aox27) as markers for species identification. From the
seven microsatellite loci three (LS57, Aox23 and Aox45) have shown a tetrasomic
profile. The most polymorphic locus for the broodstock was LS-57 with 12
alleles in population, followed by Aox45, LS-68 and LS-54 with 10 alleles. For offspring,
the most polymorphic locus was Aox 23 with 11 alleles. Other loci presented a lower
level of polymorphism range between 2 to 8 alleles identified for the analyzed
individuals. The average observed and expected heterozygosities were 0.429 and
0.413 in the broodstock and 0.500 and 0.423 in the offspring, respectively. The
preliminary data showed that selected allozymes and microsatellite markers
allow identification of the stellate sturgeon from Bulgarian farms and could be applied to test and control the broodstocks used for restocking programmes.

Keywords

Sterlet, Microsatellite, Allozymes, Identi-fication, Restocking

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