MOTILITY EVALUATION AND CRYOPRESERVATION OF FISH SPERM EXPOSED BY WATER-BORNE AND FOOD-BORNE BORON

Year 2018, Volume: 4 Issue: 1, 12 - 19, 01.01.2018

Burak Evren İnanan , Fevzi Yılmaz

Abstract

Boron (B) is required for organisms as an essential
nutrient, whereas could be toxic in definite doses. In this study, for the
first time, the effects of water-borne and food-borne B on fish sperm motility
were initially determined and these sperm samples were cryopreserved in liquid
nitrogen aiming to reveal how the quality of these samples are affected by
cryopreservation. 12 mature male goldfish (Carassius
auratus) individuals for each treatment performed in triplicate for 45 days
were used in the study. Fish were fed with diets containing 1 mg, 5 mg and 10
mg of B per kg feed for foodborne experiments. In waterborne experiments, fish
were kept in aquariums containing 1 mg/L, 10 mg/L and 20 mg/L of B. In both
fresh and cryopreserved samples, the motility durations (s) and percentages of
the sperm samples, vitality of spermatozoa (%) were determined. In the fresh
samples, percentages of sperm motility and viability in the both B treatments
decreased compared to the control, while increasing B concentration. After
cryopreservation, the sperm samples in 5 mg of B per kg feed treatment has
shown higher duration of sperm motility. Therefore, it is shown that feed-borne
B could improve sperm resistance to cold shock.

Keywords

Boron, Carassius auratus, Sperm motility, Sperm vitality, Cryopreservation

References

• Alavi, S.M., Cosson, J. (2006). Sperm Motility in Fishes. (II) Effects of Ions and Osmolality: a Review. Cell Biology International, 30(1), 1-14.
• Ardó, L., Yin, G., Xu, P., Váradi, L., Szigeti, G., Jeney, Z., Jeney, G. (2008). Chinese Herbs (Astragalus membranaceus and Lonicera japonica) and Boron Enhance the Non-Specific Immune Response of Nile tilapia (Oreochromis niloticus) and Resistance Against Aeromonas hydrophila. Aquaculture, 275, 26-33.
• Bagenal, T.B., Tesch, F.W. (1978). Age and growth. In: T.B. Bagenal (Ed.), Methods for Assessment of Fish Production in Fresh Waters (p. 101-136). Oxford: Blackwell Scientific Publications. ISBN 0632001259
• Başaran, N., Duydu, Y., Bolt, H.M. (2012). Reproductive Toxicity in Boron Exposed Workers in Bandirma. Turkey Journal of Trace Elements in Medicine and Biology, 26, 165-167.
• Björndahl, L., Söderlund, I., Kvist, U. (2003). Evaluation of the One-Step Eosin-Nigrosin Staining Technique for Human Sperm Vitality Assessment. Human Reproduction, 18(4), 813-816.
• Blevins, D.G., Lukaszewski, K.M. (1994). Proposed Physiologic Functions of Boron in Plants Pertinent to Animal and Human Metabolism. Environmental Health Perspectives, 102(7), 31-33.
• Blom, E. (1950). A One-Minute Live-Dead Sperm Stain by means of Eosin- Nigrosin. Fertility and Sterility, 1, 176-177.
• Bolt, H.M., Başaran, N., Duydu, Y. (2012). Human environmental and occupational exposures to boric acid: reconciliation with experimental reproductive toxicity data. Journal of Toxicology and Environmental Health, Part A, 75(8-10), 508-514.
• Borges, A., Siqueira, S.R., Jurinitz, D.F., Zanini, R., Amaral, F., Grillo, M.L., Oberst, E.R., Wassermann, G.F. (2005). Biochemical Composition of Seminal Plasma and Annual Variations in Semen Characteristics of Jun-dia Rhamdia quelen (Quoy and Gaimard, Pimelodidae). Fish Physiology and Biochemistry, 31, 45-53.
• Bustos-Obregon, E., Olivares, C. (2012). Boron as Testicular Toxicant in Mice (Mus domesticus). International Journal of Morphology, 30(3), 1106-1114.
• Cakmak, I., Römheld, V. (1997). Boron Deficiency-induced Impairments of Cellular Functions in Plants. Plant and Soil, 193, 71-83.
• Chao, N., Liao, I.C. (2001). Cryopreservation of Finfish and Shellfish Gametes and Embryos. Aquaculture, 197, 161-189.
• Devirian, T.A., Volpe, S.L. (2003). The Physiological Effects of Dietary Boron. Critical Reviews in Food Science and Nutrition, 43, 219-231.
• Duydu, Y., Başaran, N., Üstündağ A., Aydın, S., Ündeğer, Ü., Ataman, O.Y., Aydos, K., Düker, Y., Ickstadt, K., Waltrup, B.S., Golka, K., Bolt, H.M. (2012). Assessment of DNA Integrity (COMET assay) in Sperm Cells of Boron-exposed Workers. Archives of Toxicology, 86, 27-35.
• Fort, D.J., Stover, E.L., Strong, P.L., Murray, F.J. (1999). Effect of Boron Deprivation on Reproductive Parameters in Xenopus laevis. The Journal of Trace Elements in Experimental Medicine, 12, 187-204.
• Genç, T.O., İnanan, B.E., Yabanlı, M., Yılmaz, F. (2015). The Aggregation of Boron on the Tissues of Gold Fish (Carassius auratus Linnaeus, 1758). Turkish Journal of Agriculture - Food Science and Technology, 3(6), 498-503.
• Goldbach, H.E., Yu, Q., Wingender, R., Schulz, M., Wimmer, M., Findeklee, P., Baluska, F. (2001). Rapid Response Reactions of Roots to Boron Deprivation. Journal of Plant Nutrition and Soil Science, 164, 173-181.
• Horváth, A., Miskolczi, E., Urbanyi, B. (2003). Cryopreservation of Common Carp Sperm. Aquatic Living Resources, 16, 457-460.
• Hunt, C.D. (1994). The Biochemical Effects of Physiologic Amounts of Dietary Boron in Animal Nutrition Models. Environmental Health Perspectives, 102(7), 35-42.
• Kabu, M., Akosman, M.S. (2013). Biological Effects of Boron. Reviews of Environmental Contamination and Toxicology, 225, 57-75.
• Ku, W.W., Chapin, R.E., Wine, R.N., Gladen, B.C. (1993). Testicular toxicity of boric acid (BA): relationship of dose to lesion development and recovery in the F344 rat. Reproductive Toxicology, 7, 305-319.
• Liu, P., Wang, C., Hu, J., Xun, L., Robbins, W.A., Wu, G. (2005). Preliminary study on pregnancy outcome of spouses of boron workers. Chinese Journal of Public Health, 21(5), 567-568.
• Magyary, I., Urbanyi, B., Horvath, L. (1996). Cryopreservation of Common Carp (Cyprinus carpio L.) Sperm II. Optimal Conditions for Fertilization. Journal of Applied Ichthyology, 12, 117-119.
• Nielson, F.H. (2008). Is Boron Nutritionally Relevant? Nutrition Reviews, 66, 183-191.
• Okay, O., Güçlü, H., Soner, E., Balkas, T. (1985). Boron Pollution in the Simav River, Turkey and Various Method of Boron Removal. Water Research, 19(7), 857-862.
• Öğretmen, F., Gölbaşi, S., İnanan, B.E., Kizak, V., Kayim, M. (2014). Use of Clove Oil and Eugenol to Anesthetize Fingerling Shabut Barbus grypus. North American Journal of Aquaculture, 76(1), 9-13.
• Park, M., Li, Q., Shcheynikov, N., Zeng, W.Z., Muallem S. (2004). NaBC1 Is a Ubiquitous Electrogenic Na+-coupled Borate Transporter Essential for Cellular Boron Homeostasis and Cell Growth and Proliferation. Molecular Cell, 16(3), 331-341.
• Pegg, D.E. (2007). Principles of Cryopreservation. Methods in Molecular Biology, 368, 39-57.
• Recepoğlu, O., Beker, U. (1991). A Preliminary Study of Boron Removal From Kızıldere/Turkey Geothermal Waste Water. Geothermics, 20(1/2), 83-89.
• Rowe, R.I., Eckhert, C.D. (1999). Boron Is Requıred For Zebrafısh Embryogenesıs. Journal of Experimental Biology 202, 1649-1654.
• Rurangwa, E., Kime, D.E., Ollevier, F., Nash, J.P. (2004). The Measurement of Sperm Motility and Factors Affecting Sperm Quality in Cultured Fish. Aquaculture, 234, 1-28.
• Samman, S., Naghii, M.R., Lyons Wall, P.M. & Verus, A.P. (1998). The Nutritional and Metabolic Effects of Boron in Humans and Animals. Biological Trace Element Research, 66, 227-235.
• Scialli, A.R., Bonde J.P., Brüske-Hohlfeld, I., Culver, B.D., Li, Y., Sullivan, F.M. (2010). An Overview of Male Reproductive Studies of Boron With an Emphasis on Studies of Highly Exposed Chinese Workers. Reproductive Toxicology, 29, 10-24.
• Simonnot, M., Castel, C., Nicolaiè, M., Rosin, C., Sardin, M., Jauffret, H. (2000). Boron Removal From Drınkıng Water Wıth a Boron Selective Resin: Is The Treatment Really Selective? Water Research, 34(1), 109-116.
• Song, D., Huang, L. (1987). The Design of a 200 m3/d Deboronation System and its Field trials on Xisha Islands. Water Treatment, 2, 141-147.
• Strum, L.M.G. (1978). Aspects of the biology of Scomberomorus maculatus (Mitchill) in Trinidad. Journal of Fish Biology, 13, 155-172.
• Suquet, M., Dreanno, C., Petton, B., Normant, Y., Omnes, M.H., Billard, R. (1998). Long Term Effects of the Cryopreservation of Turbot (Psetta maxima) Spermatozoa. Aquatic Living Resources, 11, 45-48.
• Woods, W.G. (1994). An Introduction to Boron: History, Sources, Uses and Chemistry. Environmental Health Perspectives, 102(7), 5-11.