Investigation of Salmonella spp. in Healthy and Diarrheic Cats

Year 2018, Volume: 3 Issue: 1, 42 - 47, 30.04.2018

Ahmet Zenginer , Belgi Diren Sığırcı , Baran Çelik , Arzu Funda Bağcığil

https://doi.org/10.35229/jaes.387578

Abstract

Salmonellosis can
be seen both in human and many species of animals. There are many studies on
farm animals and especially in poultry farming in our country, but studies on
companion animals, which have been a big part of urban life recently, are more limited.
In this study, it is aimed to investigate the presence of Salmonella spp. with
bacteriological and molecular methods (PCR).

For this
purpose, a total of 300 rectal swabs were collected from many different
provinces of Istanbul, including healthy and diarrheic cats fed in houses or
living freely on the streets. For bacteriological examinations, swab specimens
were seeded on selective media following the pre-enrichment step. Suspected
colonies were purified and then identified by conventional methods. All swab
specimens were also investigated by PCR for presence of Salmonella spp.
specific gene regions.

As a result of
bacteriological investigations, no Salmonella
spp. was isolated from any specimen. Positivity was detected in one specimen in
PCR result. 

Keywords

Cat, Salmonella spp., culture, PCR

Sağlıklı ve İshalli Kedilerde Salmonella spp. Varlığının Araştırılması

Abstract

 Salmonelloz gerek insan gerekse birçok hayvan türünde görülebilmektedir. Ülkemizde çiftlik hayvanlarında ve özellikle de kanatlı yetiştiriciliğinde çok fazla araştırma olmakla birlikte son yıllarda şehir hayatının büyük bir parçası olmuş eşlikçi hayvanlardaki çalışmalar daha sınırlıdır. Bu çalışmada kedilerde Salmonella türlerinin varlığının bakteriyolojik ve moleküler yöntemler (PCR) ile araştırılması amaçlanmıştır.

Bu amaçla, İstanbul’un farklı birçok ilçesinde, sağlıklı ve ishalli toplam 300 adet kediden rektal svab toplanmıştır. Bakteriyolojik incelemeler için, svab örneklerinin ön zenginleştirme aşamasını takiben selektif besiyerlerine ekimleri yapılmıştır. Şüpheli koloniler saflaştırıldıktan sonra konvansiyonel yöntemler ile identifikasyonları yapılmıştır.  Tüm svab örnekleri aynı zamanda PCR ile de Salmonella spp. spesifik gen bölgelerinin varlığı yönünden incelenmiştir.

Bakteriyolojik incelemeler sonucunda hiçbir örnekten Salmonella spp. izole edilmemiştir. PCR sonucunda bir örnekte pozitiflik saptanmıştır. Sonuç olarak çalışmada Salmonella spp. izolasyon oranı dünya genelinde yapılan çalışmalarla kıyaslandığında düşük bulunmuştur. Hastalık etkeninin zoonotik özelliği de düşünüldüğünde sonuçlar umut vericidir

Keywords

Salmonella spp., PCR, kedi, kültür

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