Mastitis İzolatı Staphylococcus aureus Suşlarında Antibiyotik Dirençliliğinin ve Bazı Virülens Genlerinin Araştırılması

Year 2019, Volume: 4 Issue: 2, 182 - 187, 31.08.2019

Merve Gizem Sezener , Arzu Fındık Volkan Enes Ergüden , Serhan Akgöz , Timur Gülhan , Alper Çiftci

https://doi.org/10.35229/jaes.571977

Cited By: 4

Abstract

Bu çalışmada mastitis izolatı Staphylococcus aureus suşlarının
antibiyotik dirençliliğinin ve bazı virülens genlerinin araştırılması amaçlandı.
Çalışma kapsamında 56 adet S. aureus izolatı incelendi. İzolatlar fenotipik
yöntemlerle ve PCR ile identifiye edildi. İzolatların okzasilin, vankomisin,
teikoplanin, trimetoprim-sulfometaksazol, tetrasiklin, penisilin G, sefaperazon
ve amoksisilin-klavulonik asite karşı antibiyotik dirençlilikleri Kirby Bauer
disk difüzyon yöntemi ile belirlendi. S. aureus izolatlarında virülenste
rol oynadığı bilinen coa ve spa genlerinin varlıkları ve polimorfizmleri
PCR ile araştırıldı.

İzolatların 7, 5, 4, 5, 4, 3, 5 ve 9 adedinin sırasıyla 8,
7, 6, 5, 4, 3, 2 ve 1 antibiyotiğe karşı dirençli oldukları belirlendi. Ondört
izolat ise araştırılan tüm antibiyotiklere duyarlı olarak bulundu. İzolatların 49 adedi 9 farklı
profilde coa polimorfizmi gösterdi. Yedi izolatın ise coa geni
içermediği belirlendi. İncelenen 56 adet izolatın spa geni içerdiği ve 4
farklı profilde oldukları saptandı.

Sonuç olarak, mastitis kökenli S. aureus suşlarında
belirlenen antibiyotik direncinin halk sağlığı yönünden bir risk teşkil edebileceği
kanısına varıldı.

Keywords

coa, mastitis, PCR, S. aureus

References

• Anonim (2019). Türkiye İstatistik Kurumu Hayvancılık İstatistikleri. http://www.tuik.gov.tr/PreTablo.do?alt_id=1002 [Erişim Tarihi: 29.05.2019].
• Aslantaş, Ö., Demir, C. & Türütoğlu, H. (2007). Coagulase gene polymorphism of Staphylococcus aureus isolated from subclinical bovine mastitis. Turkish Journal of Veterinary and Animal Science, 31, 1–5.
• Boynukara, B., Gürtürk, G. & Gülhan, T. (1999). Comparison of latex agglutination tests with protein a, clumping factor and coagulase tests for identification of staphylococci isolated from avian. Eastern Journal of Medicine, 4, 58–60.
• Bray, D.R., & Shearer, J.K. (1994). Milking machine and mastitis control handbook. University of Florida, Cooperative Extension Service, Institute of Food and Agricultural Sciences. Circular. p:1136.
• Clinical Laboratory Standards Institute (2018). Performance Standards for Antimicrobial Susceptibility Testing, 28th edition: Informational Supplement. CLSI document M100-S28. Clinical and Laboratory Standards Institute, Wayne, PA, USA.
• Ciftci, A., Onuk, E.E., Fındık, A., Yıldırım, T. & Sogut, M.U. (2009). Molecular typing of Staphylococcus aureus strains from ovine mastitis by pulsed-field gel electrophoresis and polymerase chain reaction. Journal of Veterinary Diagnostic Investigations, 21 (6), 849–853.
• Fox, L.K., Bayles, K.W. & Bohach, G.A. (2001). Staphylococcus aureus Mastitis. In: Staphylococcus aureus Infection and Disease, ed. Honeyman, A.L., Friedman, H. And Bendinelli, M., Kluwer Academic /Plenum Publishers, New York, pp. 271–293.
• Frenay, H.M.E., Theelen, J.P.G., & Schouls, L.M. (1994). Discrimination of epidemic and nonepidemic methicillin-resistant Staphylococcus aureus strains on the basis of protein A gene polymorphism. Journal of Clinical Microbiology, 32, 846–847.
• Hallin, M., Deplano, A. & Denis, O. (2007). Validation of pulsed-field gel electrophoresis and spa typing for long-term, nationwide epidemiological surveillance studies of Staphylococcus aureus infections. Journal of Clinical Microbiology, 45, 127–133.
• Hartstein, A.I. & Mulligan, M.E. (1986). Methicillin-resistant Staphylococcus aureus. In: Glen Mayhall C, ed. Hospital epidemiology and infection control. Maryland: Williams and Wilkins, 290–306.
• Hashimoto, H., Inoue, M. & Hayashi, I. (1994). A survey of Staphylococcus aureus for typing and drugresistance in various areas of Japan during 1992 and 1993 [Japanese]. Japanese Journal of Antibiotics, 47, 618–626.
• Jevons, M.P. (1961). Celbenin-resistant staphylococci. British Medical Journal, 1, 124–125.
• Koneman, E.W., Allen, S.D., Janda, W.M., Schreckenberger, P.C., Winn, W.C., Procop, G. & Woods, G. (2005). Color Atlas and Textbook of Diagnostic Microbiology. Sixth edition. Philadelphia: Lippincott Co., 700–711.
• Montesinos, I., Salido, E. & Delgado, T. (2002). Epidemiologic genotyping of methicillin-resistant Staphylococcus aureus by pulsed-field gel electrophoresis at a university hospital and comparison with antibiotyping and protein A and coagulase gene polymorphisms. Journal of Clinical Microbiology, 40, 2119–2125.
• Murray, P.R. (2003). Manual of clinical microbiology, 8th ed. ASM Press, Washington, DC.
• Nagase, N., Shimizu, A, & Kawano, J. (2002). Characterization of Staphylococcus aureus strains isolated from bovine mastitis in Japan. Journal of Veterinary and Medical Science, 64, 1169–1172.
• Scherrer, D., Corti, S. & Muehlherr, J.E. (2004). Phenotypic and genotypic characteristics of Staphylococcus aureus isolates from raw bulk-tank milk samples of goats and sheep. Veterinary Microbiology, 101, 101–107.
• Schmitz, F.J. & Jones, M.E. (1997). Antibiotics for treatment of infections caused by MRSA and elimination of MRSA carriage. What are the choices? International Journal of Antimicrobial Agents, 9, 1–19.
• Su, C., Herbelin, C. & Frieze, N. (1999). Coagulase gene polymorphism of Staphylococcus aureus isolates from dairy cattle in different geographical areas. Epidemiologt and Infection, 122, 329–336.
• Zhang, K., Sarling, J. & Chow, B.L. (2004). New quadriplex PCR assay for detection of methicillin resistance and simultaneous discrimination of S. aureus from coagulase-negative staphylococci. Journal of Clinical Microbiology, 42, 4947–4955.