Farklı Sucul Sistemlerden İzole Edilen Bakterilerin Antibiyotik Dirençliliklerinin Karşılaştırılması

Year 2021, Volume: 6 Issue: 1, 25 - 30, 31.03.2021

Neslihan Güngör , Zeynep Zehra İpek , Akif Er , Şevki Kayış

https://doi.org/10.35229/jaes.804414

Cited By: 1

Abstract

Bu çalışmada Doğu Karadeniz Bölgesi’nde iki farklı sucul sistemde (deniz ve tatlı su) bazı balıklardan izole edilen bakterilerde antibiyotik dirençliliği karşılaştırılmıştır. Denizel ortamdan Rize sahillerinden örneklenen, denizatı (Hippocampus guttulatus), mezgit (Merlangius merlangus), levrek (Dicentrarchus labrax), istavrit (Trachurus mediterraneus), palamut (Sarda sarda), gökkuşağı alabalığı (Oncorhynchus mykiss) ve karides (Philocheras trispinosus) olmak üzere 7 farklı konaktan toplamda 15 bakteri izolatı kullanılmıştır. Tatlı su örnekleri olarak ise Artvin ilinde yer alan Deriner Baraj Gölü’nde, 6 farklı sazan türlerinden izole edilen toplam 32 adet bakteri izolatı kullanılmıştır. İzolatların, Ampisilin (AM10μg), Gentamisin (CN10μg), Oksitetrasiklin (T30μg), Amoksisilin- Klavulanik Asit (AMC10μg), Enrofloksasin (ENR5μg), Trimetoprim/Sulfametoksazol (SXT25μg), Florfenikol (FFC30μg), Sulfametoksazol (SMZ25μg), Eritromisin (E15μg) olmak kaydıyla 9 farklı antibiyotiğe karşı dirençlilik durumları disk difüzyon yöntemiyle belirlenmiştir. İzolatlar içerisinde Aeromonas ve Pseudomonas cinsi bakterilerin ayrıca antibiyotik dirençliliği irdelenmiştir (MAR). Farklı sucul sistemlerden izole edilen bakterilerin çoğul antibiyotik direnç indeksleri irdelendiğinde, tüm sistemlerde MAR indeksi eşik değer olan 0,2’den yüksek kaydedilmiştir. Denizel kökenli izolatlar Ampisilin ve Enrofloksasin dışında tüm antibiyotiklere karşı daha dirençli bulunmuştur.

Keywords

antibiyotik, bakteri, balık, direnç, sucul sistemler

Supporting Institution

recep tayyip erdoğan üniversitesi bilimsel araştırma projeleri koordinatörlüğü

Project Number

2014.103.02.04

Comparison of Antibiotic Resistance of Bacteria Isolated from Different Aquatic Systems

Abstract

In this study, antibiotic resistance of bacteria isolated from some fish in two different aquatic systems (sea and fresh water) in the Eastern Black Sea Region was compared. A total of 15 bacterial isolates from 7 different hosts, including seahorse (Hippocampus guttulatus), haddock (Merlangius merlangus), sea bass (Dicentrarchus labrax), horse mackerel (Trachurus mediterraneus), bonito (Sarda sarda), rainbow trout (Oncorhynchus mykiss) and shrimp (Philocheras trispinosus), were used in marine environment. In fresh water samples, a total of 32 bacterial isolates isolated from 6 different carp species Deriner Dam Lake in Artvin province, were used. The resistance of the isolates to 9 different antibiotics including Ampicillin (AM10μg), Gentamicin (CN10μg), Oxytetracycline (T30μg), Amoxicillin-Clavulanic Acid (AMC10μg), Enrofloxacin (ENR5μg), Trimethoprim / Sulfamethoxazole (SXT25μg), Florphenicol, (FFC30μg), Sulfamethoxazole (SMZ25μg), Erythromycin (E15μg) was determined by disk diffusion method. Among the isolates, bacteria of the genus Aeromonas and Pseudomonas were also investigated for antibiotic resistance (MAR). When the multiple antibiotic resistance indices of bacteria isolated from different aquatic systems are examined, the MAR index in all systems was recorded higher than the threshold value of 0.2. Marine isolates were more resistant to all antibiotics except Ampicillin and Enrofloxacin.

Keywords

Antibiotic, aquatic systems, bacteria, fish, resistance

Project Number

2014.103.02.04

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