Determination of the Presence of Klebsiella pneumoniae and Phenotypic Antibiotic Resistance Profiles in Budgerigars and Parrots

Year 2021, Volume: 6 Issue: 3, 372 - 375, 28.09.2021

Ilgın Kekeç , Barış Halaç , Beren Başaran Seyyal Ak

https://doi.org/10.35229/jaes.938912

Abstract

In this study, it was aimed to investigate the presence of Klebsiella pneumoniae and phenotypically carbapenemase, extended-spectrum β-lactamase (ESBL), acquired-AmpC beta-lactamase, and multiple antibiotic resistance of the isolates in the faeces of budgerigars and parrots. A total of 96 faecal samples belonging to 54 budgerigars and 42 parrots were used in the study. Cultivation was performed on various media for the identification of Klebsiella pneuomiae from the collected stool samples. Biochemical properties of the suspect isolates were determined using conventional methods. Besides, antibiotic susceptibility tests and ESBL, carbapenem, acquired AmpC screening and confirmation tests were applied to the identified isolates to phenotypically determine beta-lactam resistance. Beta-lactam, aminoglycoside, fluoroquinolone, tetracycline, chloramphenicol, and sulphonamide groups were used to determine the multidrug resistance. Isolates with resistance to 3 or more of the antibiotic and Sulphonamide groups were accepted as multidrug-resistant isolates. Klebsiella pneumoniae was isolated from 2 (3.7%) of 54 budgerigars, and 1 (2.3%) of 42 parrot faecal samples from 3 (3.1%) of 96 faecal samples in total. No phenotypic resistance was detected in any of the isolates as a result of screening and confirming tests for ESBL, carbapenem, and acquired-AmpC resistance to determine phenotypic antibiotic resistance of the isolates. Multidrug resistance was detected in only one isolate. The presence of multi-resistant Klebsiella pneumoniae in cage birds that have close relationships with humans has been revealed.

Keywords

Budgerigar, Klebsiella pnemoniae, multidrug resistance, parrot

Muhabbet Kuşu ve Papağanlarda Klebsiella pneumonia Varlığının ve Fenotipik Direnç Profillerinin Saptanması

Abstract

Bu çalışmada, muhabbet kuşu ve papağan dışkılarında Klebsiella pneumoniae varlığının ve fenotipik karbepenemaz, genişletilmiş spektrumlu β-laktamaz, kazanılmış- AmpC, beta-laktam ve çoklu antibiyotik direncinin belirlenmesi amaçlanmıştır. Çalışmada, 54’ü muhabbet kuşuna, 42’si papağana ait toplam 96 dışkı örneği kullanıldı. Toplanan dışkı örneklerinden K. pneuomiae, çeşitli besiyerleri kullanılarak izole edildi. Şüpheli izolatların biyokimyasal özellikleri konvansiyonel yöntemlerle belirlendi. Ayrıca beta-laktam direncini fenotipik olarak belirlemek için belirlenen izolatlara antibiyotik duyarlılık testleri ve ESBL, karbapenem, edinilmiş AmpC tarama ve doğrulama testleri uygulandı. Çoklu ilaç direncini belirlemek için beta-laktam, aminoglikozid, florokinolon, tetrasiklin, kloramfenikol ve sülfonamid grupları kullanıldı. 3 veya daha fazla antibiyotik ve sülfonamid grubuna dirençli izolatlar çoklu ilaç direncine sahip izolatlar olarak kabul edildi. Klebsiella pneumonia, 54 muhabbet kuşu dışkı örneğinin 2’sinden (%3,7), 42 papağan dışkı örneğinin 1’inden (%2,3) olmak üzere toplamda 96 dışkı örneğinin 3’ünden (%3,1) izole edildi. İzolatların fenotipik antibiyotik direncini belirlemek için ESBL, karbapenem ve edinilmiş AmpC direncinin taranması ve doğrulanması sonucunda izolatların hiçbirinde fenotipik direnç saptanmadı. Yalnızca bir izolatta çoklu ilaç direnci tespit edildi. İnsanlarla yakın ilişkileri olan kafes kuşlarında çoklu ilaç direncine sahip Klebsiella pneumoniae'nin varlığı ortaya konuldu.

Keywords

Çoklu ilaç direnci, Klebsiella pneumonia, muhabbet kuşu, papağan

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