Tissue-Specific Ameliorative Effect of Resveratrol on Oxidative Stress Parameters and Blood Lipid Profile of Mice Exposed to Cadmium

Year 2022, Volume: 7 Issue: 4, 493 - 501, 31.12.2022

Derya Kocamaz , Oğuz Merhan , Dinçer Erdağ , Hale Ergin Eğritağ , Kadir Bozukluhan

https://doi.org/10.35229/jaes.1178638

Abstract

Cadmium can cause oxidative damage in organisms through overproduction of free radicals and suppression of the antioxidant defense system. Resveratrol is known as a nutraceutical with antioxidant properties accepted to have protective effects to the toxicity of heavy metals. In this study, we investigated if resveratrol could overwhelm the toxic effect of sub-acute cadmium exposure. Animals were divided to the following groups: 1) Control, 2) Vehicle control, 3) Cadmium (Cd), 4) Resveratrol (Res), 5) Exposure to both Cd and Res (Cd+Res). Increased lipid peroxidation and total sialic acids were determined in testis and lung tissues of mice exposed to Cd with the decreased in paraoxonase (PON1) level and GSH amount. Interestingly, we also found an increased lipid peroxidation and NO levels in lung tissue of mice exposed to Res and Cd + Res, but not in testis tissue. Moreover, increased triglycerides, total cholesterol, very low-density lipoproteins, and low-density lipoproteins as well as reduced high-density lipoproteins were found in mice exposed to Cd and Cd + Res compared to controls. Our results revealed that cadmium induces oxidative damage in tissues, via increased lipid peroxidation, total sialic acids and decreased antioxidant levels as well as disturbs lipid profile. Moreover, our findings indicate that resveratrol may act as “ameliorative” factor against the cadmium exposure in only testis tissue. Thus, we may suggest that ameliorative effect of resveratrol may vary depending on the exposure dose, exposure duration and exposed tissues of animals to the heavy metals.

Keywords

Antioxidant defense system, Heavy Metals, Lipid Profile, Natural polyphenols, Oxidative Stress

Supporting Institution

The Scientific Research Committee of Kafkas University

Project Number

2016-TS-33

Thanks

This study was supported by Scientific Research Committee of Kafkas University (2016-TS-33). A part of this study was presented as an oral presentation at the EJONS VI-International Conference on Mathematics, Engineering, Natural and Medical Sciences, and II. Multidisciplinary Studies Congress.

Kadmiyuma Maruz Bırakılan Farelerin Oksidatif Stres Parametreleri ve Kan Lipid Profili üzerinde Resveratrolun Dokuya Özgü İyileştirici Etkisi

Abstract

Kadmiyum serbest radikallerin fazla üretimi ve antioksidan savunma sisteminin baskılanması yoluyla oksidatif hasara neden olabilir. Antioksidan özelliklere sahip bir nutrasötik olarak bilinen resveratrolün ağır metallerin toksisitesine karşı koruyucu etkileri olduğu kabul edilmektedir. Çalışmamızda resveratrolün sub-akut kadmiyum maruziyetinin toksik etkilerini bastırıp bastıramayacağı araştırılmıştır. Deney hayvanları 1) Kontrol, 2) Aracı kontrol, 3) Kadmiyum (Cd), 4) Resveratrol (Res) 5) Kadmiyum + Resveratrol (Cd+Res) olacak şekilde gruplara ayrılmıştır. Kadmiyuma maruz kalan farelerin testis ve akciğer dokularında artan lipid peroksidasyonu ve total sialik asit düzeylerinin yanı sıra azalan PON1 düzeyi ve GSH miktarı belirlenmiştir. İlginç bir şekilde, Res ve Cd+Res uygulanan farelerin akciğer dokularında artan lipid peroksidasyonu ve NO düzeyi belirlenmesine rağmen, testis dokularında benzer etkiler belirlenmemiştir. Ayrıca, Cd ve Cd+Res uygulanan farelerde kontrol grubu ile karşılaştırıldığında kanda trigliserit, total kolesterol, çok düşük yoğunluklu lipoproteinler ve düşük yoğunluklu lipoproteinlerin artmasının yanı sıra yüksek yoğunluklu lipoproteinler azaldığı saptanmıştır. Sonuçlarımız kadmiyumun lipid peroksidasyonunu ve total siyalik asit miktarını artırarak, antioksidan düzeylerini azaltarak ve aynı zamanda lipid profilini de etkileyerek dokularda oksidatif hasarı indüklediğini göstermektedir. Ayrıca, bulgularımız resveratrolün sadece testis dokusunda kadmiyum maruziyetine karşı “iyileştirici” bir faktör olarak hareket edebileceğini göstermektedir. Bu nedenle, resveratrolün iyileştirici etkisinin organizmaların ağır metallere maruz kalma dozuna, maruz kalma süresine ve maruz kalınan dokuya bağlı olarak değişiklik gösterebileceğini söyleyebiliriz.

Keywords

Antioksidan savunma sistemi, Ağır Metaller, Lipid Profili, Doğal polifenoller, Oksidatif Stres

Project Number

2016-TS-33

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